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http://dx.doi.org/10.5352/JLS.2022.32.9.721

Analysis of Intestinal Microbial Communities of Topshell (Turbo cornutus) fromCoast of Jeju Island, Korea by 16S rDNA Sequence Analysis  

Kim, Min-Sun (Jeju Fisheries Research Institute, National Institute of Fisheries Science)
Han, Song-Hun (Jeju Fisheries Research Institute, National Institute of Fisheries Science)
Choi, Jung Hwa (Jeju Fisheries Research Institute, National Institute of Fisheries Science)
Heo, Moon Soo (College of Ocean Sciences, Jeju National University)
Ko, Jun-Chul (Jeju Fisheries Research Institute, National Institute of Fisheries Science)
Publication Information
Journal of Life Science / v.32, no.9, 2022 , pp. 721-728 More about this Journal
Abstract
This study investigated the diversity of intestinal microbial communities isolated from the intestine of topshell (Turbo cornutus) from the coast of Jeju Island (Beobhwan, Seogwipo city). Pure cultivation using the standard marine agar (MA) medium showed the most significant number of clusters. Aerobic and anaerobic culture allowed isolation of strains of 1.8×105 CFU·g-1 and 0.4×10 CFU·g-1 on average, respectively. The microbial population in the topshell intestine was classified into 4 phyla, 12 families, 26 genera, and 67 species. The microbes in the topshell intestine were detected by homology with 93~100% with standard strains. The microbes in the topshell intestine consisted of Proteobacteria 39%, Firmicutes 34%, Actinobacteria 21%, and Bacteroidets 6%. The identified families were Alteromonadaceae (1), Shewanellaceae (4), Vibrionaceae (12), Phyllobacteriaeceae (1), Rhodobacteraceae (8), Bacillaceae (21), Paenibacillaceae (2), Cellulomonadaceae (1), Mycobacteriaceae (6), Nocardiaceae (4), Streptomycetaceae (3) and Flavobacteriaceae (4). Bacillus sp. and Vibrio sp. accounted for the greatest portion of the separated strains. Among the isolated microorganisms, some strains had probiotic functions.
Keywords
Intestinal microbial communities; marine bacteria; Turbo cornutus;
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