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http://dx.doi.org/10.5352/JLS.2022.32.9.678

HK Shiitake Mushroom Mycelium Exhibits Immune Functions in ConA-Treated Human Jurkat Cells Through NFAT Activation  

Oh, Tae Woo (Korea Institute of Oriental Medicine)
Moon, Yun Gu (Korea Institute of Toxicology)
Kim, Hun Hwan (College of Veterinary Medicine, Gyeongsang National University)
Kim, Gon Sup (College of Veterinary Medicine, Gyeongsang National University)
Kim, Jeong Ok (HK Biotech. Co., Ltd.)
Ha, Yeong Lae (HK Biotech. Co., Ltd.)
Publication Information
Journal of Life Science / v.32, no.9, 2022 , pp. 678-689 More about this Journal
Abstract
HK shiitake mushroom mycelium (HKSMM), containing 14% β-glucan, is a health functional food ingredient approved individually for liver health by the Ministry of Food and Drug Safety. The immune-enhancing efficacy of a 50% aqueous ethanol extract of HKSMM (designated HKSMM50) was studied in Jurkat cells activated with concanavalin A (ConA). Active hexose correlated compound (AHCC) was used as a positive control. ConA-activated Jurkat cells were treated with HKSMM50 (0, 25, 50, 100 ㎍ g/ml) or AHCC (100 ㎍ g/ml), and cultured for 3 and 6 hours. The nuclear factor of activated T cells (NFAT) protein content in the cytosol and the nucleus was measured by Western blotting. Interleukin-2 (IL-2), interferon-gamma (IFN-γ), and cyclooxygenase-2 (COX-2) were determined using enzyme-linked immunosorbent assay (ELISA) kits. HKSMM50 lowered NFAT content in the cytosol, but elevated NFAT content in the nucleus. The IL-2 and IFN-γ productions were elevated. Meanwhile, both COX-2 activity and apoptosis were suppressed. The efficacy of the AHCC treatment showed similar to those of HKSMM50 treatments. These results indicate that the HKSMM50 exhibited immune-enhancing effects in ConA-treated Jurkat cells by activation of NFAT protein, and suggest that HKSMM could be used as a health functional food ingredient to improve immune functions in humans.
Keywords
HK shiitake mushroom mycelium; IFN-${\gamma}$; IL-2; Jurkat cells; NFAT;
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