Browse > Article
http://dx.doi.org/10.5352/JLS.2022.32.8.633

Comparison of Anti-thrombosis, Antioxidant and Anti-diabetes Activities amongDifferent Cultivars of Hibiscus cannabinus L. Seeds  

Lee, Yun-Jin (Department of Food and Nutrition, Andong National University)
Kang, Deok-Gyeong (Department of Food and Nutrition, Andong National University)
Kim, Ji-Min (Department of Food and Nutrition, Andong National University)
Kim, Young-Min (Hemp & R Bio)
Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
Publication Information
Journal of Life Science / v.32, no.8, 2022 , pp. 633-640 More about this Journal
Abstract
Recently, kenaf (Hibiscus cannabinus L.) has been highlighted due to its rapid growth in dry, infertile land and its versatile applications in textiles, paper, feed, food, medicine, and phyto-remediation. In Korea, a new cultivar, 'Jangdae', which has the merit of feasible seed-gathering, was established in 2013. However, most of the kenaf seeds used are still imported, and a comparison study of useful bio-activities between Jangdae and imported seeds has not been conducted. In this study, anti-thrombosis, antioxidant and anti-diabetic activities of the ethanol extracts of the Jangdae seed (E-JS) and 8 imported cultivar seeds (E-ICS) were investigated. The E-JS has 14.4 mg/g of total polyphenol and 5.3 mg/g of total flavonoids, whereas the E-ICS has 5.9~8.7 mg/g and 0.7~1.5 mg/g, respectively. Among the 9 kenaf seed extracts, IT 181215 and IT 207877 seed extracts showed 1.92 and 1.88-folds extended thrombin time (TT), and IT 207886 seed extracts showed 3.04-folds extended activated partial thromboplastin time (aPTT). All extracts, except Hong-ma seed, showed 1.36~1.56-folds extended prothrombin time (PT). The E-JS showed strong anti-thrombosis activities with 1.46-folds (TT), 1.38-folds (PT) and 2.37-folds (aPTT) compared to those of solvent controls. The E-JS has the strongest antioxidant activities among the seed extracts, with 0.48 mg/ml of RC50 for ABTS cation and 0.27 mg/ml of RC50 for nitrite scavenging. Meanwhile, the E-JS and IT 181215 extracts showed strong inhibition against α-glucosidase with 10.3~10.6% inhibition at 0.5 mg/ml. Our results suggest the E-JS has potential as an anti-thrombosis, antioxidant and anti-diabetic agent.
Keywords
Anti-thrombosis; anti-oxidant; anti-diabetic; Hibiscus cannabinus L.; 'Jangdae' cultivar;
Citations & Related Records
Times Cited By KSCI : 9  (Citation Analysis)
연도 인용수 순위
1 Pyo, S. J, Kang, D. G, Jung, C. and Sohn, H. Y. 2020. Anti-thrombotic, Anti-oxidant and haemolysis activities of six edible insect species. Foods 9, 401. doi: 10.3390/foods9040401.   DOI
2 Singleton, V. L., Orthofer, R. and Lamuela-Raventos, R. M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol. 299, 152-178.   DOI
3 Wang, J., Xiong, X. and Feng, B. 2014. Aspirin resistance and promoting blood circulation and removing blood stasis: Current situation and prospectives. Evid. Based Complement. Alternat. Med. 2014, 954863.
4 Wei, Q., Ji, X. Y., Xu, F., Li, Q. R. and Yin, H. 2015. Chemical constituents from leaves of Hibiscus syriacus and their alpha-glucosidase inhibitory activities. Zhong Yao Cai 38, 975-979.
5 Wong, Y. H., Tan, W. Y., Tan, C. P., Long, K. and Nyam, K. L. 2014. Cytotoxic activity of kenaf (Hibiscus cannabinus L.) seed extract and oil against human cancer cell lines. Asian Pac. J. Trop. Biomed. 4(Suppl 1), S510-515.   DOI
6 Ifie, I., Ifie, B. E., Ibitoye, D. O., Marshall, L. J. and Williamson, G. 2018. Seasonal variation in Hibiscus sabdariffa (Roselle) calyx phytochemical profile, soluble solids and alpha-glucosidase inhibition. Food Chem. 261, 164-168.   DOI
7 Jorge, A. P., Horst, H., de Sousa, E., Pizzolatti, M. G. Silva, F. R. 2004. Insulinomimetic effects of kaempferitrin on glycaemia and on 14 C-glucose uptake in rats oleusmuscle. ChmicoBiol. Interact. 14, 989-996.
8 Kang, C. H., Yoo, Y. J., Choi, K. H., Kim, H. J., Shin, Y. K., Lee, G. J., Ko, D. Y., Song, Y. J. and Kim, C. K. 2014. Analysis of ecotype, growth and development, yield and feed value of Kenaf (Hibiscus cannabinus L.) genetic resources. Kor. J. Plant Res. 27, 556-566.   DOI
9 Kang, S. Y., Kwon, S. J., Jeong, S. W., Kim, J. B., Kim, S. H. and Ryu, J. 2016. An improved kenaf cultivae 'Jangdae' with seed harvesting in Korea. Kor. J. Breed Sci. 48, 349-354.   DOI
10 Kim, M. S., Shin, W. C., Kang, D. K. and Sohn, H. Y. 2016. Anti-thrombosis activity of sinapic acid isolated from the lees of bokbunja wine. J. Microbiol. Biotechnol. 26, 61-65.   DOI
11 Kwon, C. S., Sung, H. J. and Sohn, H. Y. 2019. Anti-thrombosis activities of the root extract of Moringa oleifera Lam. Microbiol. Biotechnol. Lett. 47, 20-24.   DOI
12 Lee, M. H., Sung, H. J., Kwon, C. S. and Sohn, H. Y. 2018. Anti-coagulation and anti-platelet aggregation activities of black ginger (Kaempferia parviflora). J. Life Sci. 28, 1068-1075.   DOI
13 Martinez, M., Weisel, J. W. and Ischiropoulos, H. 2013. Functional impact of oxidative posttranslational modifications on fibrinogen and fibrin clots. Free Radic. Biol. Med. 65, 411-418.   DOI
14 Kumar, D., Kumar, H., Vedasiromoni, J. R. and Pal, B. C. 2012. Bio-assay guided isolation of alpha-glucosidase inhibitory constituents from Hibiscus mutabilis leaves. Phytochem. Anal. 23, 421-425.   DOI
15 Lee, J. Y., Velusamy, V., Koo, J. Y., Ha, H. K., Kim, D. S., Kim, J. B., Kim, S. H. and Kang, S. Y. 2012. Comparison of growth characteristics and chemical composition of kenaf (Hibiscus cannabinus L.) varieties as a potential forage crop. J. Crop Sci. 57, 132-136.
16 Masuko, T., Minami, A., Iwasaki, N., Majima, T., Nishimura, S. and Lee, Y. C. 2005. Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Anal. Biochem. 339, 69-82.   DOI
17 Matarrese, P., Straface, E., Pietraforte, D., Gambardella, L., Vona, R., Maccaglia, A., Minetti, M. and Malorni, W. 2005. Peroxynitrite induces senescence and apoptosis of red blood cells through the activation of aspartyl and cysteinyl proteases. FASEB J. 19, 416-418.
18 Szczeklik A, Musial, J., Undas, A., Swadzba, J., Gora, P. F., Piwowarska, W. and Duplaqa, M. 1996. Inhibition of thrombin generation by aspirin is blunted in hypercholesterolemia. Arterioscler. Thromb. Vasc. Biol. 16, 948-954.   DOI
19 Pyo, S. J., Lee, Y. J., Kang, D. G., Son, H. J., Park, G. H., Park, J. Y. and Sohn, H. Y. 2020. Antimicrobial, antioxidant, and anti-diabetic activities of Rodgersia podophylla. J. Life Sci. 30, 298-303.   DOI
20 Ryu, J., Ha, B. K., Kim, D. S., Kim, J. B., Kim, S. H. and Kang, S. Y. 2013. Assessment of growth and seed oil composition of kenaf (Hibiscus cannabinus L.) germplasm. J. Crop Sci. Biotechnol. 16, 297-302.   DOI
21 Vishnu Prasad, C. N., Suma Mohan, S., Banerji, A. and Gopalakrishnapillai, A. 2009. Kaempferitrin inhibits GLUT4 translocation and glucose uptake in 3T3-L1 adipocytes. Biochem. Biophy. Res. Comm. 380, 39-43.   DOI
22 Wang, L., Li, L., Wang, H. and Liu, J. 2017. Study on the influence of oxidative stress on the fibrillization of fibrinogen. Sci. Rep. 7, 12429.
23 Yusri, N. M., Chan, K. W., Iqbal, S. and Ismail, M. 2012. Phenolic content and antioxidant activity of Hibiscus cannabinus L. seed extracts after sequential solvent extraction. Molecules 17, 12612-12621.   DOI
24 Kang, C. H., Lee, I. S., Go, D. Y., Kim, H. J. and Na, Y. E. 2018. The growth and yield differences in kenaf (Hibiscus cannabinus L.) in reclaimed land based on the physical types of organic materials. Kor. J. Crop Sci. 63, 64-71.   DOI
25 Kang, D. K., Lee, Y. J., Kim. Y. M. and Sohn, H. Y. 2022. Evaluation of anti-thrombosis and anti-oxidant activities of the ethanol extract of different parts of Hibiscus cannabinus L. cv 'Jangdae' J. Life Sci. 32, 155-160.
26 Kang, S. Y., Kim, P. G., Kang, Y. K., Kang, B. K., Riu, U. Z. K. and Song, H. S. 2004. Growth, yield and photosynthesis of introduced kenaf cultivars in Korea. Kor. J. Plant Res. 17, 139-146.
27 Kim, D. B., Shin, G. H., Lee, Y. J., Lee, J. S., Cho, J. H., Baik, S. O. and Lee, O. H. 2014. Assessment and comparison of the antioxidant activities and nitrite scavenging activity of commonly consumed beverages in Korea. Food Chem. 151, 58-64.   DOI
28 Kai, N. S., Nee, T. A., Ling, E. L. C., Ping, T. C., Kamariah, L. and Lin, N. K. 2015. Anti-hypercholesterolemic effect of kenaf (Hibiscus cannabinus L.) seed on high-fat diet Sprague dawley rats. Asian Pac. J. Trop Biomed. 8, 6-13.   DOI
29 Kim, J. H., Jeong, G. H., Jeong, Y. H. and Kim, T. H. 2019. Free radical scavenging and α-glucosidase inhibitory activities of the extracts of Dystaenia takesimana from Ulleung island. Kor. J. Food Preserv. 26, 246-252.   DOI
30 Kim, M. S. and Sohn, H. Y. 2016. Anti-oxidant, anti-coagulation, and anti-platelet aggregation activities of black currant (Ribes nigrum L.). J. Life Sci. 26, 1400-1408.   DOI
31 Alexopoulou, E., Papattheohari, Y., Christou, M. and Monti, A. 2013. Keanf: A multi-purpose crop for several industrial applications. Springer-verlag, London. England. pp. 1-15.
32 Bijak, M., Nowak, P., Borowiecka, M., Ponczek, M. B., Zbikowska, H. M., and Wachowicz, B. 2012. Protective effects of (-)-epicatechin against nitrative modifications of fibrinogen. Thromb. Res. 130, e123-128. doi: 10.1016/j.thromres.2012.03.017.   DOI
33 Boller, S., Soldi, C., Marques, M. C., Santos, E. P., Cabrini, D. A., Pizzolatti, M. G., Zampronio, A. R. and Otuki, M. F. 2009. Antiinflammatory effect of crude extract and isolated compounds from Baccharis illinita DC in acute skin inflammation. J. Ethnopharmacol. 130, 262-266.   DOI
34 Choi, Y. H., Kong, K. R., Kim, Y. A., Jung, K. O., Kil, J. H. Rhee, S. H. and Park, K. Y. 2003. Induction of Bax and activation of caspases during β-sitosterol-mediated apoptosis in human colon cancer cells. Int. J. Oncol. 23, 1657-1662
35 Jin, C. W., Eom, S. H., Park, H. J., Ghimeray, A. K., Yu, C. Y. and Cho, D. H. 2009. Antioxidant activity of Hibiscus cannabinus L. leaves in different growth time. Kor. J. Med. Crop Sci. 17, 21-25.
36 De Melo, G. O., Malvar Ddo, C., Vanderlinde, F. A., Rocha, F. F., Pires, P. A., Costa, E. A., de Matos, L. G., Kaiser, C. R. and Costa, S. S. 2009. Antinociceptive and anti-inflammatory kaempferol glycosides from Sedumdendroideum. J. Ethnopharmacol. 12, 4228-4232.
37 Han, S. E., Sung, K. I. and Kim, B. Y. 2004. Changes of dry matter yield and nutritive value of Kenaf (Hibiscus cannabinus. L) cultivars on different harvest dates in Chunchon area. Ann. Anim. Resour. 15, 1-7.
38 Jeong, J. S., Kim, G. M., Jeong, H. J., Kim, G. B. and Jeon, C. H. 2019. A study on the improved the hydrophobicity of torrefied biomass. Trans. Kor. Hydrogen New Energy Soc. 30, 49-57.   DOI