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http://dx.doi.org/10.5352/JLS.2022.32.3.232

Development of a Method for Producing Liposome Ascorbic acid with Increased Bio-absorption  

Cha, Ji Hyun (Department of Pharmaceutical Engineering, Silla University)
Woo, Young Min (Department of Pharmaceutical Engineering, Silla University)
Jo, Eun Sol (Hankook liposome Co., Ltd.)
Cha, Jae Young (Department of Pharmaceutical Engineering, Silla University)
Lee, Sang Hyeon (Department of Pharmaceutical Engineering, Silla University)
Lee, Keun Woo (Hankook liposome Co., Ltd.)
Kim, Andre (Department of Pharmaceutical Engineering, Silla University)
Publication Information
Journal of Life Science / v.32, no.3, 2022 , pp. 232-240 More about this Journal
Abstract
Various methods are known for preparing liposomes, the simplest being the Bangham method which has been widely used. Although it is possible to produce liposomes effectively on a small experimental level with this approach, large-scale production cannot be easily performed due to difficulties in removing the organic solvent and the size of the reactor required to form the lipid film. On the other hand, emulsion can mass produce tons of liposomes with uniform particles but has the disadvantage of a significantly low capture rate. This study therefore developed an optimal liposome processing method using heat with improved capture rate and stability, and bio-absorption experiments were performed by oral administration to SD rat alongside capture rate, particle size, and zeta potential. Through the heating method, a small and uniform liposome of about 214 nm was formed and the capture rate was 38.67%, confirming that the liposome prepared by heating has a higher capture rate than the 26.46% achieved through emulsion. Comparing blood concentrations, it showed a 1.5 to 2 fold increase in all groups, gradually decreasing until 4-12 hr. The highest blood concentration of ascorbic acid powder was about 12.017 ㎍/ml, the emulsion liposome 13.871 ㎍/ml, and the heating liposome 16.322 ㎍/ml, thereby showing an improved absorption rate.
Keywords
Bio-absorption rate; liposome; oral administration; particle size; zeta potential;
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