Browse > Article
http://dx.doi.org/10.5352/JLS.2022.32.3.222

The Effects on Melanogenesis in B16F10 Melanoma Cells and the Anti-inflammatory Activities of an Ethyl Acetate Fraction from Glechoma hederacea var. longituba  

Yeom, Hyeon-Ji (Division of Cosmetics and Biotechnology, Hoseo University)
Oh, Min-Jeong (Division of Cosmetics and Biotechnology, Hoseo University)
Chae, Jung-Woo (Gyeonggi-do Forest Environment Research Institute)
Lee, Jin-Young (Division of Cosmetics and Biotechnology, Hoseo University)
Publication Information
Journal of Life Science / v.32, no.3, 2022 , pp. 222-231 More about this Journal
Abstract
This study aimed to confirm the possibility of being used as a cosmetic material material through the verification of the whitening and anti-inflammatory activities of an ethyl acetate fraction from Glechoma hederacea var. longituba (GHEA). The observed electron donating and ABTS+ radical scavenging abilities of GHEA were 89.6% and 88.7%, respectively, at 1,000 ㎍/ml concentration, with a tyrosinase inhibitory effect of 22.3% at the same concentration. For cell viability, a rate of 80% or more was observed in all concentrations that treated GHEA on melanoma and macrophage cells. Protein and mRNA expression inhibition was measured by Western blot and RT-PCR for 25, 50, and 100 ㎍/ ml concentrations, and it was confirmed that expression decreases in a concentration-dependent manner as GHEA concentration increases. The inhibition of the whitening-related factors MITF and TRP-2 were superior following GHEA treatment than those of the control group treated with kojic acid of 100 ㎍/ml concentration. For tyrosinase, the lowest mRNA expression rate was 29.1% at 100 ㎍/ml which confirmed excellent inhibition. In analyzing the effects of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α on protein and mRNA expression, IL-6 and TNF-α showed high protein and mRNA inhibition compared to a vitamin C control group. Based on these experimental results, GHEA could be applied as a natural cosmetic material.
Keywords
Anti-inflammatory; cytokine; Glechoma hederacea var. longituba; IL-6; whitening;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 181, 1199-1120.   DOI
2 Cho, Y. H., Kim, H. J., Kim, D. I., Jang, J. Y., Kwak, J. H., Shin, Y. H., Cho, Y. G. and An, B. J. 2015. Effect of garlic (Allium sativum L.) stems on inflammatory cytokines, iNOS and COX-2 expressions in Raw 264.7 cells induced by lipopolysaccharide. Kor. J. Food Preserv. 22, 613-621.   DOI
3 Hyun, J. M., Park, K. J., Kim, S. S., Park, S. M., Lee, Y. J. and An, H. J. 2015. Antioxidant and anti-inflammatory effects of solvent fractions from the peel of the native Jeju citrus 'Hongkyool' and 'Pyunkyool'. J. Life Sci. 25, 1132-1138.   DOI
4 Lee, H. J. 2004. Inhibitory effect of nitric oxide productionof Lysimachiae christinae. Master's Thesis Dissertation, Wonkwang University, Jeonrabukdo, Korea.
5 Kim, M. K. and Kim, D. Y. 2015. Anti-inflammatory effect of barley leaf ethanol extract in LPS-stimulated RAW264.7 macrophage. Kor. J. Food Preserv. 22, 735-743.   DOI
6 Jo, J. B., Kim, M. U., Lee, E. H., Kim, Y. J., Cho, E. B., Kang, I. K. and Cho, Y. J. 2018. Whitening effect of extracts from Matricaria chamomilla L. with B16F10 melanoma cells. J. Appl. Biol. Chem. 61, 267-273.   DOI
7 Kim, A. R., Jung, M. C., Jeong, H. I., Song, D. G., Seo, Y. B., Jeon, Y. H., Park, S. H., Shin, H. S., Lee, S. L. and Park, S. N. 2018. Antioxidative and cellular protective effects of Lysimachia christinae Hance extract and fractions. Appl. Chem. Eng. 29, 176-184.   DOI
8 Kim, D. H., Moon, Y. S., Park, T. S., Hwang, J. Y. and Son, J. H. 2013. Potent whitening activity of Aruncus dioicus extract in B16F10 melanoma cell by suppression of melanin biosynthesis. Kor. J. Hort. Sci. Technol. 31, 813-820.
9 Yu, H. A. and Kim, C. D. 2017. Applicability of Lindera obtusiloba flower extracts as cosmetic ingredients. Asian J. Beauty Cosmetol. 15, 132-144.   DOI
10 Woo, H. S., Lee, S. M., Heo, J. D., Lee, M. S., Kim, Y. S. and Kim, D. W. 2018. Anti-inflammatory activity of extracts of Hovenia dulcis on lipopolysaccharides-stimulated RAW 264.7 cells. Kor. J. Plant Res. 31, 466-477.   DOI
11 Kim, H. S. and Ko, K. S. 2020. Antioxidant and anti-inflammatory effects of Ginseng berry ethanol extracts as a cosmetic ingredient. Asian J. Beauty Cosmetol. 18, 389-387.   DOI
12 Lim, S. B., Park, K. T., Lee, E. H., Kim, B. O., Lee, S. H., Kang, I. K. and Cho, Y. J. 2017. Quality characteristics and biological activity of Yanggaeng with Glechoma hederacea var. longituba Nakai powder. Kor. J. Food Preserv. 24, 206-214.   DOI
13 Park, Y. H., Lim, S. H., Kim, H. Y., Kim, K. H. and Kim, S. 2009. Comparison of compounds on the essential oil in G. hederacea var. longituba Nakai by fertilizer treatment. Kor. J. Hort. Sci. Technol. 27, 171.
14 Chun, H. J., Hwang, S. G., Lee, J. S., Baek, S. H., Jeon, B. H. and Woo, W. H. 2002. Inhibitory effects of butyl alcohol extract from Caesalpinia sappan L. on melanogenesis in melan-a cells. Kor. J. Pharmacogn. 33, 130-136.
15 Hwang, C. R., Shin, J. H., Kang, M. J., Lee, S. J. and Sung, N. J. 2012. Antioxidant and antiobesity activity of solvent fractions from red garlic. J. Life Sci. 22, 950-957   DOI
16 Lee, S. H., Kim, S. Y., Kim, J. J., Jang, T. S. and Chung, S. R. 1999. The isolation of the inhibitory constitutents on melanin polymer formation from the leaves of Cercis chinensis. Kor. J. Pharmacogn. 30, 397-403.
17 Kim, P. K., Jung, K. I., Choi, Y. J. and Gal, S. W. 2017. Anti-inflammatory effects of lemon myrtle (Backhousia citriodora) leaf extracts in LPS-induced RAW 264.7 cells. J. Life Sci. 27, 986-993.   DOI
18 Lee, E. J., Seo, Y. M., Kim, Y. H., Chung, C., Sung, H. J., Sohn, H. Y., Park, J. Y. and Kim, J. S. 2019. Anti-inflammatory activities of ethanol extracts of dried Lettuce (Lactuca sativa L.). J. Life Sci. 29, 325-331.   DOI
19 Jeong, S. J., Kim, K. H. and Yook, H. S. 2015. Whitening and antioxidant activities of solvent extracts from hot-air dried Allium hookeri. J. Kor. Soc. Food Sci. Nutr. 44, 832-839.   DOI
20 Jun, Y. J., Lee, S., Heo, S. and Jin, B. S. 2019. Functional characterization of the extracts from Nipa palm, Molokhia, and Finger root for cosmetic ingredients. J. Kor. Appl. Sci. Technol. 36, 821-829.
21 Kim, M. S., Kim, K. H. and Yook, H. S. 2012. Antioxidative effects of Campanula takesimana Nakai extract. J. Kor. Soc. Food Sci. Nutr. 41, 1331-1337.   DOI
22 Kim, O. K. 2004. Antidiabetic effect of Glechoma hederacea LINNAEUS in streptozotocin-induced diabetic rats. Kor. J. Pharmacogn. 34, 300-308.
23 Kim, S., Kim, H. Y., Hwang, K. H. and Chun, I. J. 2008. Herbicidal activity of essential oil from Glechoma hederacea. Kor. J. Weed Sci. 28, 152-160.
24 Lee, G. W., Lee, J. Y. and Cho, Y. H. 2010. Whitening effect of the extracts from Juglans mandshurica. KSBB J. 25, 18-24.
25 Lee, H. J. and Lim, M. H. 2020. Whitening activities of extracts of seomaeyakssuk (Artemisia argyi H.). J. Kor. Appl. Sci. Technol. 37, 241-249.
26 Oh, H. K. 2020. Antioxidant and anti-inflammatory activities of different parts of Ixeris dentata according to extract methods. J. Kor. Appl. Sci. Technol. 37, 1567-1574.   DOI
27 Sim, J. H., Lee, K. M., Park, T., Kang, M. S., Hong, H. and Kim, S. Y. 2021. Biorenovation-assisted modifiction of Ligustrum japonicum extract for skin-whitening effect. KSBB J. 36, 30-35.   DOI
28 Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D. and Mitchell, H. B. 1987. Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47, 936-942.
29 Park, S. J., Kwon, S. P. and Rha, Y. A. 2017. Antioxidative activities and whitening effects of ethanol extract from Crataegus pinnatifida bunge fruit. J. Kor. Soc. Food Sci. Nutr. 46, 1158-1163.   DOI
30 Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26, 1231-1237.   DOI
31 You, S. H. 2017. Antioxidant activity and whitening activity of Psidium guajava leaf extract. J. Oil Appl. Sci. 34, 296-304.   DOI
32 Yagi, A., Kanbara, T. and Morinobu, N. 1987. Inhibition of mushroom-tyrosinase by Aloe extract. Planta Med. 53, 515-517.   DOI