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http://dx.doi.org/10.5352/JLS.2021.31.7.671

Lethal (2) Essential for Life [l(2)efl] Gene in the Two-spotted Cricket, Gryllus bimaculatus (Orthoptera: Gryllidae)  

Kwon, Kisang (Department of Clinical Laboratory Science, Wonkwang Health Science University)
Lee, Nuri (Departments of Anatomy & Cell Biology, College of Medicine, Chungnam National University)
Kwon, O-Yu (Departments of Anatomy & Cell Biology, College of Medicine, Chungnam National University)
Publication Information
Journal of Life Science / v.31, no.7, 2021 , pp. 671-676 More about this Journal
Abstract
A cDNA encoding the protein lethal (2) essential for life [l(2)efl] was cloned from Gryllus bimaculatus and named GBl(2)efl. This protein is composed of 189 amino acids, including an N-glycosylation site and 15 phosphorylation sites. Its predicted molecular mass is 21.19 kDa, with a theoretical isoelectric point of 6.2. The secondary structure of GBl(2)efl was predicted from the identification of random coils (56.08%), alpha helices (22.22%), extended strands (17.99%), and beta turns (3.7%) through sequence analyses. A homology analysis revealed that GBl(2)efl exhibited a high similarity with other species at the amino acid level, ranging from 52% to 69%. While GBl(2)efl mRNA expression was higher in the dorsal longitudinal flight muscle following a three-day starvation and in the Malpighian tubules following a one-day starvation, no changes in expression were detected in other tissues. Furthermore, tunicamycin-induced endoplasmic reticulum (ER) stress resulted in an approximately 1.8-fold higher expression in the fat body compared with the wild type.
Keywords
Endoplasmic reticulum (ER) stress; G. bimaculatus l(2)efl [GBl(2)efl]; lethal (2) essential for life (l(2)efl); two-spotted cricket Gryllus bimaculatus;
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