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http://dx.doi.org/10.5352/JLS.2021.31.7.654

Biochemical Properties of a Whitening Bioactive Agent Derived from Thrichoderma viridescens SW-1  

Kang, Dong Woo (Department of Pharmaceutical Engineering, Gyeongsang National University)
Kim, Pan Gil (Department of Clinical Pathology, Jinju Health College)
Kim, Sam Woong (Gene Analysis Center, Gyeongsang National University)
Bang, Kyu Ho (Department of Pharmaceutical Engineering, Gyeongsang National University)
Kim, Chul Ho (Department of Pharmaceutical Engineering, Gyeongsang National University)
Lee, Sang Won (Department of Pharmaceutical Engineering, Gyeongsang National University)
Gal, Sang Wan (Department of Pharmaceutical Engineering, Gyeongsang National University)
Publication Information
Journal of Life Science / v.31, no.7, 2021 , pp. 654-661 More about this Journal
Abstract
In this study, in order to isolate excellent whitening agents from fungal cultural broth, various fungi were collected from wild areas in South Korea and then screened for tyrosinase inhibition activity, as tyrosinase is a precursor for the biosynthesis of melanin in regulating skin color. A fungus strain that inhibits tyrosinase activity has been identified and confirmed as Trichoderma viridescens (later renamed T. viridescens SW-1) via ITS sequencing. In T. viridescens SW-1, tyrosinase inhibitory activity was strongest on day three of culture. A 5% culture broth showed a tyrosinase inhibitory activity greater than 90% and exhibited high thermostability on day three. At 10% culture broth, the accumulations of intra- and extracellular melanin were inhibited above 27.1% and 7.5%, respectively. In summary, the physical and functional properties of the tyrosinase inhibitory substances of T. viridescens SW-1 included high levels of inhibition of melanin synthesis and antioxidative activity as well as thermostability. Therefore, we suggest that the whitening substance identified from the cultural broth of T. viridescens SW-1 has potential for application as a functional cosmetic ingredient.
Keywords
Cosmetics; metabolite; Trichoderma viridescens; tyrosinase inhibitor; whitening bioactive agent;
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1 Boo, Y. C. 2020. Up- or Downregulation of melanin synthesis using amino acids, peptides, and their analogs. Biomedicines 8, 322.   DOI
2 Hsiao, N. W., Tseng, T. S., Lee, Y. C., Chen, W. C., Lin, H. H., Chen, Y. R., Wang, Y. T., Hsu, H. J. and Tsai, K. C. 2014. Serendipitous discovery of short peptides from natural products as tyrosinase inhibitors. J. Chem. Inf. Model. 54, 3099-3111.   DOI
3 KCTC (Korean Collection for Type Cultures). https://kctc.kribb.re.kr/
4 Kumari, S., Thng, S. T. G., Verma, N. K. and Gautam, H. K. 2018. Melanogenesis inhibitors. Acta dermato-venereologica 98, 924-931.   DOI
5 Ohbayashi, N. and Fukuda, M. 2020. Recent advances in understanding the molecular basis of melanogenesis in melanocytes. F1000Res. 9, F1000 Faculty Rev-608.
6 Rzepka, Z., Buszman, E., Beberok, A. and Wrzesniok, D. 2016. From tyrosine to melanin: Signaling pathways and factors regulating melanogenesis. Postepy higieny i medycyny doswiadczalnej (Online) 70, 695-708.   DOI
7 Simon, J. D., Peles, D., Wakamatsu, K. and Ito, S. 2009. Current challenges in understanding melanogenesis: Bridging chemistry, biological control, morphology, and function. Pigment Cell Melanoma Res. 22, 563-579.   DOI
8 Tsuchiya, T., Yamada, K., Minoura, K., Miyamoto, K., Usami, Y., Kobayashi, T., Hamada-Sato, N., Imada, C. and Tsujibo, H. 2008. Purification and determination of the chemical structure of the tyrosinase inhibitor produced by Trichoderma viride strain H1-7 from a marine environment. Biol. Pharm. Bull. 31, 1618-1620.   DOI
9 Yamada, K., Imada, C., Tsuchiya, T., Miyamoto, K., Tsujibo, H., Kobayasi, T. and Hamada-Sato, N. 2007. An application study of culture supernatant of a filamentous fungus isolated from marine environment as an ingredient for whitening cosmetics. J. Soc. Cosmet. Chem. Japan 41, 254-261.   DOI
10 Kim, K. Y. and Lee, N. K. 2014. Herbal extracts research trend that have effects on melanin production and control. Asian J. Beauty Cosmetol. 12, 453-461.
11 Kowichi, J., Obata, H., Pathak, M. A. and Fitzpatrick, T. B. 1974. Mechanism of depigmentation by hydroquinone. J. Invest. Dermatol. 62, 436-449.   DOI
12 Lee, Y. H., Lim N. H., Joung, M. S., Kim, J. H. and Sim, S. S. 2005. Chemical stability and whitening activity of mixture of functional ingredients. J. Soc. Cosmet. Scientists Korea 31, 295-304.
13 Lin, J. Y. and Fisher, D. E. 2007. Melanocyte biology and skin pigmentation. Nature 445, 843-850.   DOI
14 Steinho, M., Stander, S., Seeliger, S., Ansel, J. C., Schmelz, M. and Luger, T. 2003. Modern aspects of cutaneous neurogenic inflammation. Arch. Dermatol. 139, 1479-1488.   DOI
15 Olivares, C. and Solano, F. 2009. New insights into the active site structure and catalytic mechanism of tyrosinase and its related proteins. Pigment Cell Melanoma Res. 22, 750-760.   DOI
16 Parvez, S., Kang, M., Chung, H. S., Cho, C., Hong, M. C., Shin, M. K. and Bae, H. 2006. Survey and mechanism of skin depigmenting and lightening agents. Phytother. Res. 20, 921-934.   DOI
17 Slominski, A., Tobin, D. J., Shibahara, S. and Wortsman, J. 2004. Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol. Rev. 84, 1155-1228.   DOI
18 Saeedi, M., Eslamifar, M. and Khezri, K. 2019. Kojic acid applications in cosmetic and pharmaceutical preparations. Biomed. Pharmacother. 110, 582-593.   DOI
19 Schiano, M. V. 2010. Signaling pathways in melanosome biogenesis and pathology. Int J. Biochem. Cell Biol. 42, 1094-1104.   DOI
20 Videira, I. F. D. S., Moura, D. F. L. and Magina, S. 2013. Mechanisms regulating melanogenesis. An. Bras. Dermatol. 88, 76-83.   DOI
21 Wang, D. H., Ootsuki, Y., Fujita, H., Miyazaki, M., Yie, Q., Tsutsui, K. and Ogino, K. 2012. Resveratrol inhibited hydroquinone-induced cytotoxicity in mouse primary hepatocytes. Int. J. Environ. Res. Public Health 9, 3354-3364.   DOI
22 Jablonski, N. H. and Chaplin, G. 2014. Human skin pigmentation as an adaptation to UV radiation. Proc. Natl. Acad. Sci. USA. 107, 8962-8968.   DOI
23 Yoon, M. Y., Han, E. H. and Han, Y. S. 2013. A study on anti-oxidant activity and whitening action of Plantago asiatica L. seed extract. J. Kor. Soc. Beauty Art 14, 259-269.
24 Zolghadri, S., Bahrami, A., Hassan Khan, M. T., MunozMunoz. J., Garcia-Molina. F., Garcia-Canovas, F. and Saboury, A. A. 2019. A comprehensive review on tyrosinase inhibitors. J. Enzyme Inhib. Med. Chem. 34, 279-309.   DOI
25 Gillbro, J. M. and Olsson, M. J. 2011. The melanogenesis and mechanisms of skin lightening agents-existing and new approaches. Int. J. Cosmet. Sci. 33, 210-221.   DOI
26 Han, J. S. and Yi, D. H. 2012. Effects of pine needles fermentation extracts on antioxidant activity and inhibition of melanin synthesis. Asian J. Beauty Cosmetol. 10, 619-624.
27 Inoue, Y., Hasegawa, S., Yamada, T., Date, Y., Mizutani, H., Nakata, S., Matsunaga, K. and Akamatsu, H. 2013. Analysis of the effects of hydroquinone and arbutin on the differentiation of melanocytes. Biol. Pharm. Bulletin 36, 1722-1730.   DOI