Browse > Article
http://dx.doi.org/10.5352/JLS.2021.31.2.229

Antioxidant Effects of Stewartia koreana Nakai Leaves and Branch Extracts  

Kim, Hye Soo (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Park, Min Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Kim, Soo Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Kim, Bu Kyung (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Park, JunHo (Forest Research Department, Gyeongsangnam-do Forest Environment Research Institute)
Kim, DaeHyun (Forest Research Department, Gyeongsangnam-do Forest Environment Research Institute)
Cho, Soo Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Life Science / v.31, no.2, 2021 , pp. 229-236 More about this Journal
Abstract
This study was carried out to evaluate the antioxidant properties of the dried leaves and branches of Stewartia koreana Nakai. The dried leaf and branch of S. koreana were extracted with 70% ethanol at 80℃. The antioxidant activities of ethanol extracts of S. koreana leaf (EESL) and S. koreana branch (EESB) were analyzed. The total polyphenol contents in EESL and EESB were 162.57±0.9 mg of GAEs/extract g and 59.1±0.9 mg of GAEs/extract g, respectively. The flavonoid contents in EESL and EESB were 59.1±0.9 mg of QEs/extract g and 4.7±0.1 mg of QEs/extract g, respectively. EESL showed a better scavenging ability with DPPH and ABTS than EESB, at 0.4 mg/ml. Moreover, EESL were more effective according to ORAC values than EESL. The toxicity of EESL was investigated using a WST-1 assay on the human skin fibroblast cell line CCD-986sk. Therefore, EESL can be used as a potential source of functional, naturally-sourced material in cosmetics as well as food.
Keywords
Flavonoids content; ORAC value; radical scavenging ability; Stewartia koreana Nakai; total polyphenol content;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Halliwell, B. and Gutterridge, J. M. 1990. Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol. 186, 1-85.   DOI
2 Jeong, H. J., Lee, S. G., Lee, E. J., Park, W. D., Kim, J. B. and Kim, H. J. 2010. Antioxidant activity and anti-hyperglycemic activity of medicinal herbal extracts according to extraction methods. Kor. J. Food Sci. Technol. 42, 571-577.
3 Kammeyer, A. and Luiten, R. M. 2015. Oxidation events and skin aging. Ageing Res. Rev. 21, 16-29.   DOI
4 Kang, Y. H., Park, Y. K. and Lee, G. D. 1996. The nitrite scavenging and electron donating ability of phenol compounds. Kor. J. Food Sci. Technol. 28, 232-239.
5 Khalid, O. A. 2009. Oxidant/antioxidant status in obese adolescent females with Acne vulgaris. Indian J. Dermatol. 54, 36-40.   DOI
6 Kim, D. J., Oh, S. K., Yoon, M. R., Chun, A. R., Hong, H. C., Lee, J. S. and Kim, Y. K. 2010. Antioxidant compounds and antioxidant activities of the 70% ethanol extracts from brown and milled rice by cultivar. J. Kor. Soc. Food Sci. Nutr. 39, 467-473.   DOI
7 Kim, Y. J., Lee, Y. R., Cheon, J. W. and Lee, H. S. 2010. Anti-aging effect of Ligustrum japonicum extract in the human fibroblast cells. J. Soc. Cosmet. Scientists Kor. 36, 295-301.
8 Kim, M. H., Jang, J. H., Oh, M. H., Heo, J. H. and Lee, M. W. 2014. The comparison of DPPH-scavenging capacity and anti-inflammatory effects of phenolic compounds isolated from the stems of Stewartia koreana Nakai. Nat. Prod. Res. 28, 1409-1412.   DOI
9 Kim, S. C., Kwon, H. S., Kim, C. H., Kim, H. S., Lee, C. Y. and Cho, S. J. 2016. Comparison of antioxidant activities of pileus and stipe from White Beech Mushroom (Hypsizygus marmoreus). J. Life Sci. 26, 928-935.   DOI
10 Kim, S. C., Ryu, H. M., Jung, S. M., Lee, Y. H., Kim, H. S., Kim, J. O., Cho, Y. U. and Cho, S. J. 2013. Antioxidant and tyrosinase inhibitory activity of Hypsizygus marmorus (brown cultivar) methanol extracts. J. Mushroom Sci. Prod. 11, 254- 260.   DOI
11 Kim, Y. J. and Son, D. Y. 2012. Antioxidant and inhibitory effects of Korean Panax ginseng extract on pro-inflammatory mediators in LPS-stimulated RAW 264.7 macrophages. J. Kor. Soc. Food Sci. Nutr. 41, 1371-1377.   DOI
12 Lee, D. H. and Hong, J. H. 2015. Antioxidant activities of chlorella extracts and physicochemical characteristics of spray-dried chlorella powders. Kor. J. Food Preserv. 22, 591-597.   DOI
13 Lee, S., You, Y., Kim, K., Park, J., Jeong, C., Jhon, D. Y. and Jun, W. 2012. Antioxidant activities of native Gwangyang Rubus coreanus Miq. J. Kor. Soc. Food Sci. Nutr. 41, 327-332.   DOI
14 Duh, P. D., Tu, Y. Y. and Yen, G. C. 1999. Antioxidant activity of water extract of Harng jyur (Chrysanthemum morifolium Ramat). LWT-Food Sci. Technol. 32, 269-277.   DOI
15 Cao, G., Alessio, H. M. and Cutler, R. G. 1993. Oxygen-radical absorbance capacity assay for antioxidants. Free Radic. Biol. Med. 14, 303-311.   DOI
16 Cha, J. Y. 2009. Functional components and biological activities of marketing black garlic. MS Thesis. Gyeongsang National University.
17 Chen, H. Y., Lin, Y. C. and Yen, G. C. 2007. Antioxidant activity and free radical scavenging capacity of extracts from guava (Psidium guauva L.) leaves. Food Chem. 101, 686-694.   DOI
18 Francoeur, A. M. and Assalian, A. 1996. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3, 19-25.
19 Guk, M. H., Kim, D. H., Lee, C., Jeong, E. S., Choi, E. J., Lee, J. S. and Lee, T. S. 2013. Antioxidant and skin whitening effects of Inonotus obliquus methanol extract. J. Mushroom Sci. Prod. 11, 99-106.   DOI
20 Lee, T. H., Lee, G. W., Kim, C. W., Bang, M. H., Baek, N. I., Kim, S. H., Chung, D. K. and Kim, J. 2010. Stewartia koreana extract stimulates proliferation and migration of human endothelial cells and induces neovasculization in vivo. Phytother. Res. 24, 20-25.   DOI
21 Lee, T. H., Lee, G. W., Park, K. H., Mohamed, M. A., Bang, M. H., Baek, Y. S., Son, Y., Chung, D. K., Baek, N. I. and Kim, J. 2014. The stimulatory effects of Stewartia koreana extract on the proliferation and migration of fibroblasts and the wound healing activity of the extract in mice. Int. J. Mol. Med. 34, 145-152.   DOI
22 Moon, J. S., Kim, S. J., Park, Y. M., Hwang, J. S., Kim, E. H., Park, J. W., Park, I. B., Kim, S. W., Kang, S. G., Park, Y. K. and Jung, S. T. 2004. Activities of antioxidation and alcohol dehydrogenase inhibition of methanol extracts from some medicinal herbs. Kor. J. Food Preserv. 11, 201-206.
23 Ou, B., Huang, D., Hampsch-Woodill, M., Flanagan, J. A. and Deemer, E. K. 2002. Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays: a comparative study. J. Agric. Food Chem. 50, 3122-3128.   DOI
24 Ozer, A., Ergul, B. K. and Sezai, S. 2005. Oxidative stress in patients with acne vulgaris. Mediators Inflamm. 6, 380-384.
25 Park, C. K., Kim, H. J., Kwak, H. B., Lee, T. H., Bang, M. H., Kim, C. M., Lee, Y. K., Chung, D. K., Baek, N. I., Kim, J., Lee, Z. H. and Kim, H. H. 2007. Inhibitory effects of Stewartia koreana on osteoclast differentiation and bone resorption. Int. Immunopharmacol. 7, 1507-1516.   DOI
26 Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26, 1231-1237.   DOI
27 Park, S. W., Kim, S. H. and Jung, S. K. 1995. Antimutagenic effects and isolation of flavonoids from Humulus japonicus extract. Kor. J. Food Sci. Technol. 27, 897-901.
28 Lee, S. Y., Choi, H. D., Yu, S. N., Kim, S. H., Park, S. K. and Ahn, S. C. 2015. Biological activities of Mesembryanthemum crystallinum (Ice plant) extract. J. Life Sci. 25, 638-645.   DOI
29 Parrado, J., Bougria, M., Ayala, A., Castano, A. and Machado, A. 1999. Effects of aging on the various steps of protein synthesis: fragmentation of elongation factors 2. Free Radic. Biol. Med. 26, 362-370.   DOI
30 Prior, R. L., Hoang, H., Gu, L., Wu, X., Bacchiocca, M., Howard, L., Hampsch-Woodill, M., Huang, D., Ou, B. and Jacob, R. 2003. Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORAC)) of plasma and other biological and food samples. J. Agr. Food Chem. 51, 3273-3279.   DOI
31 Roh, H. J., Noh, H. J., Na, C. S., Kim, C. S., Kim, K. H., Hong, C. Y. and Lee, K. R. 2015. Phenolic compounds from the leaves of Stewartia pseudocamellia Maxim. and their whitening activities. Biomol. Ther. 23, 283-289.   DOI
32 Singleton, V. L. and Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am. J. Enol. Vitic. 16, 144-158.
33 Tomas, C., Tsai, B. S. and Basil, M. H. 2008. Cosmeceutical agents: A comprehensive review of the literature. Clin. Med. Dermatol. 1, 1-20.
34 Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 181, 1199-1200.   DOI
35 Yen, G. C. and Hsieh, C. L. 1998. Antioxidant activity of extracts from Du-zhong (Eucommia ulmoides) toward various lipid peroxidation models in vitro. J. Agric. Food Chem. 46, 3952-3957.   DOI
36 Tominaga, H., Ishiyama, M., Ohseto, F., Sasamoto, K., Hamamoto, T., Suzuki, K. and Watanabe, M. 1999. A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Anal. Commun. 36, 47-50.   DOI
37 Toshinori, B. and Chikako, N. 2012. Impact of reactive oxygen species on keratinocyte signaling pathways. J. Dermatol. Sci. 68, 3-8.   DOI
38 Yang, C. S., Landau, J. M., Hung, M. T. and Newmark, H. L. 2001. Inhibition of carcinogenesis by dietary polyphenolic compounds. Annu. Rev. Nutr. 21, 381-406.   DOI
39 Aitken, R. J., Buckingham, D. and Harkiss, D. 1993. Use of xanthine oxidase free radical generating system to investigate the cytotoxic effects of reactive oxygen species on human spermatozoa. J. Reprod. Fertil. 97, 441-450.   DOI
40 Bae, J. J. and Kwak, J. H. 2015. Phenolic compounds from the twigs of Stewartia pseudocamellia. Kor. J. Pharmacogn. 46, 303-308.
41 Branen, A. L. 1975. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. Am. Oil Chem. Soc. 52, 59-63.   DOI