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http://dx.doi.org/10.5352/JLS.2021.31.2.199

Antioxidant and Anticancer Activities of Euonymus porphyreus Extract in Human Lung Cancer Cells A549  

Jin, Soojung (Core-Facility Center for Tissue Regeneration, Dong-eui University)
Oh, You Na (Core-Facility Center for Tissue Regeneration, Dong-eui University)
Son, Yu Ri (Core-Facility Center for Tissue Regeneration, Dong-eui University)
Bae, Soobin (Core-Facility Center for Tissue Regeneration, Dong-eui University)
Park, Jung-ha (Core-Facility Center for Tissue Regeneration, Dong-eui University)
Kim, Byung Woo (Blue-Bio Industry Regional Innovation Center, Dong-eui University)
Kwon, Hyun Ju (Core-Facility Center for Tissue Regeneration, Dong-eui University)
Publication Information
Journal of Life Science / v.31, no.2, 2021 , pp. 199-208 More about this Journal
Abstract
Euonymus porphyreus, a species of plant in the Celastraceae family, is widely distributed in East Asia, especially in Southern China. The botanical characteristics of E. porphyreus have been reported, but its antioxidative and anticancer activities remain unclear. In this study, we evaluated the antioxidative and anticancer effects of ethanol extracts of E. porphyreus (EEEP) and the molecular mechanism of its anticancer activity in human lung adenocarcinoma A549 cells. The total polyphenol and flavonoid compound contents from EEEP were 115.42 mg/g and 23.07 mg/g, respectively. EEEP showed significant antioxidative effects with a concentration at 50% of the inhibition (IC50) value of 11.09 ㎍/ml, as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. EEEP showed cytotoxic activity by increasing the SubG1 cell population of A549 cells in a dose-dependent manner. Apoptosis in A549 cells treated with EEEP was evident due to increased apoptotic cells and apoptotic bodies, as detected by Annexin V and 4,6-diamidino-2-phenylindole (DAPI) staining, respectively. EEEP-induced apoptosis resulted in increased expression of the First apoptosis signal (Fas), p53, and Bax, with decreased expression of Bcl-2 and subsequent activation of caspase-8, -9, and caspase-3, leading to cleavage of poly (ADP-ribose) polymerase (PARP). Collectively, these results suggest that EEEP may exert an anticancer effect by inducing apoptosis in A549 cells through both intrinsic and extrinsic pathways.
Keywords
A549 cells; anticancer; antioxidant; apoptosis; Euonymus porphyreus;
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