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http://dx.doi.org/10.5352/JLS.2021.31.1.52

Distribution of Subgroups in Pseudomonas syringae pv. actinidiae Biovar 3 Strains Isolated from Korea  

Lee, Young Sun (Department of Biology, Sunchon National University)
Kim, Gyoung Hee (Department of Plant Medicine, Sunchon National University)
Jung, Jae Sung (Department of Biology, Sunchon National University)
Publication Information
Journal of Life Science / v.31, no.1, 2021 , pp. 52-58 More about this Journal
Abstract
Pseudomonas syringae pv. actinidiae, which causes bacterial canker in kiwifruit, is divided into five biovars (1, 2, 3, 5, 6) on the basis of genetic characteristics and toxin productivity. Among them, biovar 3 is responsible for the current global outbreak, and has been isolated in Korea since 2011. Biovar 3 strains isolated from Korea are subdivided into six genetically different lineages (subgroup I, IV, V, VI, VII, and VIII) based on random amplified polymorphic DNA (RAPD) analysis. In this work, the subgroup-specific sequence characterized amplified region (SCAR) primers were developed from sequenced differential RAPD bands. Distribution of the subgroups of the biovar 3 strains collected in Korea from 2011-2017 were examined using these subgroup-specific primer sets. Among the 54 strains tested, 35 strains (64.8%) belonged to subgroup V, 9 strains (16.7%) belonged to subgroup IV, 4 strains (7.4%) belonged to subgroup VI, 3 strains (5.6%) belonged to subgroup VII, 2 strains (3.7%) belonged to subgroup VIII, and 1 (1.9%) strain belonged to subgroup I. Strains belonging to subgroups IV, V, and VI were shown to be related to strains isolated from China, New Zealand, and Chile, respectively. The study revealed that the biovar 3 strains in Korea are genetically diverse and are estimated to have been introduced through pollen sourced from foreign countries.
Keywords
Bacterial canker; biovar 3; kiwifruit; Pseudomonas syringae pv. actinidiae;
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