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http://dx.doi.org/10.5352/JLS.2020.30.8.680

The Regulation of Early Senescence-related SM22 Protein in Human Dermal Fibroblasts by Hovenia dulcis Thunberg Fractions  

Sim, Bo Ram (Beauty care Technology, Dongnam Health University)
Publication Information
Journal of Life Science / v.30, no.8, 2020 , pp. 680-687 More about this Journal
Abstract
Several studies have proved Hovenia dulcis extracts to be an effective antidote for alcoholism, and they are frequently used in the clinical treatment of alcoholic liver disease. The purpose of this study was to investigate the anti-oxidant and anti-senescent activities of 80% methanol fractions of H. dulcis Thunberg in human dermal fibroblast (HDF) cells. The ethanol ethyl acetate fraction showed the highest inhibition of 2,2-diphenyl-1-picryhydrazyl radical scavenging (RC50=3.6±0.8 ㎍/ml) as well as the highest total phenol content (417.2antiholic liver disease. Extracts from e & Tech., Sungkyunkwan University, progressed in the HDF cells, SM22 expression (p<0.001) and β-galactosidase staining intensity increased (p<0.001). An increase in SM22 expression is associated with slower aging which suggests that its expression is high in early aging. HDF treatment using the ethyl acetate fraction significantly increased production of EGF (p<0.01) and PDGF-BB (p<0.01) while SHH expression was reduced (p<0.05). These results suggest that an 80% ethyl acetate fraction of H. dulcis can suppress early aging and demonstrate the potential application of the fraction as a natural anti-senescence agent.
Keywords
Anti-senescence; DPPH; ${\beta}$-galactosidase; Hovenia dulcis; Sm22;
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