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http://dx.doi.org/10.5352/JLS.2020.30.6.555

Absorbance as Simple Indicator for Polyphenol Content and Antioxidant Activity of Honey  

Pyo, Su-Jin (Department of Food and Nutrition, Andong National University)
Kim, Jong-Sik (Department of Biological Science, Andong National University)
Lee, Dong Hee (Industry Academy Cooperation Foundation, Andong National University)
Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
Publication Information
Journal of Life Science / v.30, no.6, 2020 , pp. 555-562 More about this Journal
Abstract
This study analyzed the total polyphenol (TP), total flavonoid (TF), and protein content, the Absorbance at 400 nm (A400), and the antioxidant and hemolytic activities of 150 Korean honey products, including 41 chestnut (CH), 42 acacia (AH), 62 multi-floral (MH), and five Styrax japonica (TH) varieties. Our results showed that the components and antioxidant activities of honey are dependent on botanical origin rather than farming area or farmer. CH showed the highest levels of TP (88.6±29.8 mg/100 g) and TF (1.20±0.82 mg/100 g), whereas TH had the highest protein (21.5±5.1 mg/100 g). A400 was the highest in CH (0.161±0.044). All of the honey products exhibited negligible hemolytic activity against human red blood cells up to 1 mg/ml. Potent radical scavenging activities for 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,2-azobis(3-ethylbenzothiazoline-6- sulfonate) (ABTS), nitrite and reducing power were also observed in CH. Correlation coefficients (CCs) between analysis parameters were calculated and the highest was identified between TP and ABTS scavenging activity (0.726). The CCs between A400 and TP and A400 and ABTS scavenging activity were 0.644 and 0.661, respectively, suggesting that A400 could be used as a quality indicator for the polyphenol content and antioxidant activity of particular honeys. Future research on polyphenol by flower origin and the identification of compounds for A400 is necessary.
Keywords
Absorbance 400 nm; anti-oxidation; domestic honey; origin of flower;
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