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http://dx.doi.org/10.5352/JLS.2020.30.4.370

Protective Effect of Dendropanax morbifera Leaf Extract on CCl4-induced Oxidative Damage in HepG2 Cells  

Park, Se-Ho (Department of Applied Chemistry, Kumoh National Institute of Technology)
Lee, Jae-Yeul (Department of Applied Chemistry, Kumoh National Institute of Technology)
Jhee, Kwang-Hwan (Department of Applied Chemistry, Kumoh National Institute of Technology)
Yang, Seun-Ah (Department of Food Science and Technology, Keimyung University)
Publication Information
Journal of Life Science / v.30, no.4, 2020 , pp. 370-378 More about this Journal
Abstract
This study aimed to investigate the hepatoprotective effect of Dendropanax morbifera (D. morbifera) leaf hot-water extract on carbon tetrachloride (CCl4)-treated HepG2 cells. Treatment with D. morbifera leaf hot-water extract increased the cell viability of CCl4-treated HepG2 cells without inducing cytotoxicity. The levels of alanine transaminase (ALT) and aspartate transaminase (AST) released by CCl4-treated cells were 27.6 U/L and 52.4 U/L, respectively, and were significantly higher than those in untreated control cells (10.0 U/L and 15.2 U/L, respectively). Moreover, the level of γ-glutamyl transpeptidase (GGT) was 5.4 times higher, while that of glutathione was 44.0% lower in CCl4-treated cells than in control cells. However, treatment with D. morbifera leaf hot-water extract resulted in a dose-dependent decrease in the levels of ALT, AST, and GGT, and an increase in the level of glutathione. Moreover, the malondialdehyde (MDA) content in CCl4-treated HepG2 cells was effectively reduced after treatment with D. morbifera leaf hot-water extract. Additionally, overproduction of intracellular lipids induced by CCl4 treatment was effectively inhibited by D. morbifera leaf hot-water extract treatment. Furthermore, DCFDA staining showed that overproduction of reactive oxygen species (ROS) induced by CCl4 treatment was effectively reduced by treatment with D. morbifera leaf hot-water extract. Our results indicate that owing to its beneficial effects, D. morbifera leaf extract has considerable potential as a functional food material for liver protection.
Keywords
Carbon tetrachloride; Dendropanax morbifera; hepatoprotective; malodialdehyde; reactive oxygen species;
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Times Cited By KSCI : 11  (Citation Analysis)
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1 Ahn, J. C., Kim, M. Y., Kim, O. T., Kim, K. S., Kim, S. H., Kim, S. H. and Hwang, B. 2002. Selection of the high yield capacity of Hwangchil lacquer and identification of aromatic components in essential oil of Dendropanax morbifera Lev. Kor. J. Med. Crop Sci. 10, 126-131.
2 Ahn, M. J., Kim, J. T., Hong, S. H., Kim, J. G., Ko, H. N., Lee, N. H., Kim, G. O. and Shin, T. K. 2018. Black radish (Raphanus sativus L. var. niger) extract mediates its hepatoprotective effect on carbon tetrachloride-induced hepatic injury by attenuating oxidative stress. J. Med. Food 21, 866-875.   DOI
3 Akanitapichat, P., Phraibung, K., Nuchklang, K. and Prompitakkul, S. 2010. Antioxidant and hepatoprotective activities of five eggplant varieties. Food Chem. Toxicol. 48, 3017-3021.   DOI
4 An, N. Y., Kim, J. E., Hwang, D. and Ryu, H. K. 2014. Antidiabetic effects of aqueous and ethanol extract of Dendropanax morbifera Leveille in streptozotocin-induced diabetes model. J. Nutr. Health 47, 394-402.   DOI
5 Bae, D. H., Kim, J. H., Lee, S. Y., Choi, E. J., Jung, M. A., Jeong, C. S., Na, J. R., Kim, J. J. and Kim, S. O. 2015. Hepatoprotective effects of aqueous extracts from leaves of Dendropanax morbifera leveille against alcohol-induced hepatotoxicity in rats and in vitro anti-oxidant effect. Food Sci. Biotechnol. 24, 1495-1503.   DOI
6 Mo, J. H. and Oh, S. J. 2013. Tyrosinase inhibitory activity and melanin production inhibitory activity of the methanol extract and fraction from Dendropanax morbifera Lev. Kor. J. Aesthet. Cosmetol. 11, 275-280.
7 Ni, X. and Wang, H. 2016. Silymarin attenuated hepatic steatosis through regulation of lipid metabolism and oxidative stress in a mouse model of nonalcoholic fatty liver disease (NAFLD). Am. J. Transl. Res. 8, 1073-1081.
8 Pareek, A., Godavarthi, A., Issarani, R. and Nagori, B. P. 2013. Antioxidant and hepatoprotective activity of Fagonia schweinfurthii (Hadidi) Hadidi extract in carbon tetrachloride induced hepatotoxicity in HepG2 cell line and rats. J. Ethnopharmacol. 150, 973-981.   DOI
9 Pareek, A., Godavarthi, A. and Nagori, B. P. 2013. In vitro hepatoprotective activity of Corchorus depressus L. against CCl4 induced toxicity in HepG2 cell line. Pharmacogn. J. 5, 191-195.   DOI
10 Park, M. K., Lee, Y. J. and Kang, E. S. 2005. Hepatoprotective effect of Cheonnyuncho (Opuntia humifusa) extract in rats treated carbon tetrachloride. Kor. J. Food Sci. Technol. 37, 822-826.
11 Park, S. A., Park, J., Park, C. I., Jie, Y. J., Hwang, Y. C., Kim, Y. H., Jeon, S. H., Lee, H. M., Ha, J. H., Kim, K. J. and Park, S. N. 2013. Cellular antioxidant activity and whitening effects of Dendropanax morbifera leaf extract. Kor. J. Microbiol. Biotechnol. 41, 407-415.   DOI
12 Park, T. H., Park, S. H., Lee, J. Y., Yang, S. A. and Jhee, K. H. 2019. The effect of fermented extracts of Korean Dendropanax morbifera Leveille on hair growth. J. Life Sci. 29, 455-460.   DOI
13 Reddy, M. K., Reddy, A. G., Kumar, B. K., Madhuri, D., Boobalan, G. and Reddy, M. A. 2017. Protective effect of rutin in comparison to silymarin against induced hepatotoxicity in rats. Vet. World 10, 74-80.   DOI
14 Rhee, I. J. and An, J. Y. 2003. Hepatoprotective effects of water extract of Liriopis Tuber on carbon tetrachloride-induced hepatotoxicity in rats. Kor. J. Pharmacogn. 34, 166-171.
15 Richar, O. R., Eric, A. G. J., James, A. D. and Robert, L. W. 1989. Mechanism of carbon tetrachloride toxicity. Pharmacol. Therapeut. 43, 139-154.   DOI
16 Shah, V. N., Shah, M. B. and Bhatt, P. A. 2011. Hepatoprotective activity of Punarnavashtak kwath, an Ayurvedic formulation, against CCl4-induced hepatotoxicity in rats and on the HepG2 cell line. Pharm. Biol. 49, 408-415.   DOI
17 Jeny, C., Goy, J., Fechner, J., Loeper, J. and Emerit, J. 1986. Study of lipid peroxidation by the assay of malondialdehyde in human and experimental atheroma. Presse Med. 15, 1131-1133.
18 Shen, T., Li, X., Hu, W., Zhang, L., Xu, X., Wu, H. and Ji, L. 2015. Hepatoprotective effect of phenylethanoid glycosides from Incarvillea compacta against CCl4-induced cytotoxicity in HepG2 cells. J. Kor. Soc. Appl. Biol. Chem. 58, 617-625.   DOI
19 Simon, H. U., Haj-Yehia, A. and Levi-Schaffer, F. 2000. Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis 5, 415-418.   DOI
20 Slamenova, D., Kozics, K., Hunakova, L., Melusova, M., Navarova, J. and Horvathova, E. 2013. Comparison of biological processes induced in HepG2 cells by tert-butyl hydroperoxide (t-BHP) and hydroperoxide ($H_2O_2$): The influence of carvacrol. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 757, 15-22.   DOI
21 Im, K. H., Jang, S. B. and Yoo, D. Y. 2015. Anti-cancer effects of Dendropanax morbifera extract in MCF-7 and MDA-MB-231 cells. J. Kor. Obstet. Gynecol. 28, 26-39.   DOI
22 Jain, M., Kapadia, R., Jadeja, R. N., Thounaojam, M. C., Devkar, R. V. and Mishra, S. H. 2011. Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf. Asian Pac. J. Trop. Biomed. 6, 443-447.   DOI
23 Kim, J. H., Bae, D. H., Lee, U. and Heo, H. J. 2016. Ameliorating effect of water extract from Dendropanax morbifera Lev. on memory dysfunction in streptozotocin-induced diabetic rats. Kor. J. Food Sci. Technol. 48, 275-283.   DOI
24 Lee, S. H., Lee, H. S., Park, Y. S., Hwang, B., Kim, J. and Lee, H. Y. 2002. Screening of immune activation activities in the leaves of Dendropanax morbifera Lev. Kor. J. Med. Crop Sci. 10, 109-115.
25 Krithikaa, R., Mohankumarb, R., Vermaa, R. J., Shrivastavc, P. S., Mohamadd, I. M., Gunasekarand, P. and Narasimhanb, S. 2009. Isolation, characterization and antioxidative effect of phyllanthin against CCl4-induced toxicity in HepG2 cell line. Chem. Biol. Interact. 181, 351-358.   DOI
26 Kumar, N., Rai, A., Reddy, N. D., Shenoy, R. R., Mudgal, J., Bansel, P., Mudgal, P. P., Arumugam, K., Udupa, N., Sharma, N. and Rao, C. M. 2019. Improved in vitro and in vivo hepatoprotective effects of liposomal silymarin in alcohol-induced hepatotoxicity in Wistar rats. Pharmacol. Rep. 71, 703-712.   DOI
27 Lee, S. G., Lee, S. H. and Park, E. J. 2015. Antimicrobial and antioxidant activities of ethanol leaf extract of Dendropanax morbiferus Lev. Kor. J. Food Cook Sci. 31, 515-523.   DOI
28 Lee, S. M., Park, S. Y., Jang, G. S. and Ly, S. Y. 2008. The protective effects of ethanol extract of wild simulated ginseng on carbon tetrachloride induced acute hepatic injury in mouse. Kor. J. Nutr. 41, 701-710.
29 Lee, S. Y., Choi, E. J., Bae, D. H., Lee, D. W. and Kim, S. 2015. Effects of 1-tetradecanol and $\beta$-sitosterol isolated form Dendropanax morbifera Lev. on skin whitening, moisturizing and preventing hair loss. J. Soc. Cosmet. Sci. Kor. 41, 73-83.   DOI
30 Lim, D. W., Kim, H., Park, S. Y., Park, W. H. and Kim, J. E. 2017. Study of the suppressive effect and its mechanism of Amomum cardamomum L. on free fatty acid-induced liver steatosis. J. Physiol. Pathol. Kor. Med. 31, 159-166.   DOI
31 Yang, S. A., Garcia, C. V. and Lee, J. W. 2017. Volatile compounds and antiproliferative effects of Dendropanax morbifera on HepG2 cells. J. Life Sci. 27, 561-566.   DOI
32 Cho, A. R., Kim, D. G., Kang, J. R., Kang, M. J. and Shin, J. H. 2019. Antioxidant and liver protecting activity of combined extracts of medicinal herbs. J. Kor. Soc. Food Sci. Nutr. 48, 1195-1204.   DOI
33 Guo, Y., Cao, L., Zhao, Q., Zhang, L., Chen, J., Liu, B. and Zhao, B. 2016. Preliminary characterizations, antioxidant and hepatoprotective activity of polysaccharide from Cistanche deserticola. Int. J. Biol. Macromol. 93, 678-685.   DOI
34 Videla, L. A. 2009. Oxidative stress signaling underlying liver disease and hepatoprotective mechanisms. World J. Hepatol. 1, 72.   DOI
35 Zeashan, H., Amresh, G., Singh, S. and Rao, C. V. 2009. Hepatoprotective and antioxidant activity of Amaranthus spinosus against $CCl_4$ induced toxicity. J. Ethnopharmacol. 125, 364-366.   DOI