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http://dx.doi.org/10.5352/JLS.2020.30.2.156

Biodegradation of Phenol by Comamonas testosteroni DWB-1-8 Isolated from the Activated Sludge of Textile Wastewater  

Kwon, Hae Jun (School of Life Science and Biotechnology (BK21 Plus KNU Creative Bioresearch Group), Kyungpook National University)
Choi, Doo Ho (School of Life Science and Biotechnology (BK21 Plus KNU Creative Bioresearch Group), Kyungpook National University)
Kim, Mi Gyeong (School of Life Science and Biotechnology (BK21 Plus KNU Creative Bioresearch Group), Kyungpook National University)
Kim, Dong-Hyun (School of Life Science and Biotechnology (BK21 Plus KNU Creative Bioresearch Group), Kyungpook National University)
Kim, Young Guk (School of Life Science and Biotechnology (BK21 Plus KNU Creative Bioresearch Group), Kyungpook National University)
Yoon, Hyeokjun (National Institute of Biological Resources)
Kim, Jong-Guk (School of Life Science and Biotechnology (BK21 Plus KNU Creative Bioresearch Group), Kyungpook National University)
Publication Information
Journal of Life Science / v.30, no.2, 2020 , pp. 156-161 More about this Journal
Abstract
Since industrialization, the production and utilization of various chemicals has contributed to improving the quality of our lives, but the subsequent discharge of massive waste is inevitable, and environmental pollution is becoming more serious every day. Exposure to chemicals as a result of environmental pollution is having a negative effect on human health and the ecosystem, and cleaning up the polluted environment that can affect our lives is a very important issue. Toxic aromatic compounds have been detected frequently in soil, groundwater, and wastewater because of the extensive use of oil products, and phenol, which is used to produce synthetic resins, textiles, and dyes, is one of the major pollutants, along with insecticides and preservatives. Phenol can cause dyspnea, headache, vomiting, mutation, and carcinogenesis. Phenol-degrading bacterium DWB-1-8 was isolated from the activated sludge of textile wastewater; this strain was identified as Comamonas testosteroni by 16S rRNA gene sequencing. The optimal culture conditions for the cell growth and degradation of phenol were 0.7% K2HPO4, 0.6% NaH2PO4, 0.1% NH4NO3, 0.015% MgSO4·7H2O, 0.001% FeSO4·7H2O, an initial pH of 7, and a temperature of 30℃. The strain was also able to grow by using other toxic compounds, such as benzene, toluene, or xylene (BTX), as the sole source of carbon.
Keywords
Bioremediation; Comamonas testosteroni; phenol; textile wastewater;
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1 Ahmed, A. M., Nakhla, G. F. and Farooq, S. 1995. Phenol degradation by Pseudomonas aeruginosa. J. Environ. Sci. Health A 30, 99-107.
2 Boon, M., Goris, J., Vos, P. D., Verstraete, W. and Top, E. V. 2000. Bioaugmentation of activated Sludge by indigenous 3-chloroaniline degrading Comamonas testosteroni strain, 12gfp. Appl. Environ. Microbiol. 66, 2906-2913.   DOI
3 Cho, K. S., Lee, S. M., Shin, M. J., Park, S. Y., Lee, Y. R., Jang, E. Y. and Son, H. J. 2014. Isolation and characteristics of a phenol-degrading bacterium, Rhodococcus pyridinovorans P21. J. Life Sci. 24, 988-994.   DOI
4 Der Yang, R. and Humphrey, A. E. 1975. Dynamic and steady state studies of phenol biodegradation in pure and mixed cultures. Biotechnol. Bioeng. 17, 1211-1235.   DOI
5 Edwards, C. A. 2002. Assessing the effects of environmental pollutants on soil organisms, communities, processes and ecosystems. Eur. J. Soil Biol. 38, 225-231.   DOI
6 Yoon, S. H., Ha, S. M., Kwon, S., Lim, J., Kim, Y., Seo, H. and Chun, J. 2017. Introducing EzbioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int. J. Syst. Evol. Microbiol. 67, 1613-1617.   DOI
7 Zaitsev, G. M., Uotila, J. S., Tsitko, I. V., Lobanok, A. G. and Salkinoja-Salonen, M. S. 1995. Utilization of halogenated benzenes, phenols, and benzoates by Rhodococcus opacus GM-14. Appl. Environ. Microbiol. 61, 4191-4201.   DOI
8 Ettinger, M., Ruchhoft, C. and Lishka, R. 1951. Sensitive 4-aminoantipyrine method for phenolic compounds. Anal. Chem. 23, 1783-1788.   DOI
9 Fiamegos, Y., Stalikas, C. and Pilidis, G. 2002. 4-Aminoantipyrine spectrophotometric method of phenol analysis: Study of the reaction products via liquid chromatography with diode-array and mass spectrometric detection. Anal. Chim. Acta 467, 105-114.   DOI
10 Folsom, B. R. and Chapman, P. J. 1991. Performance characterization of a model bioreactor for the biodegradation of trichloroethylene by Pseudomonas cepacia G4. Appl. Environ. Microbiol. 57, 1602-1608.   DOI
11 Kim, S. B., Kim, C. K., Kim, H. S., Lee, C. H., Shin, K. S., Kwon, G. S., Yoon, B. D. and Oh, H. M. 1996. Isolation and characterization of phenol-degrading Candida tropicalis PW-51. Kor. J. Appl. Microbiol. Biotechnol. 24, 743-748.
12 Pai, S. L., Hsu, Y. L., Chong, N. M., Sheu, C. S. and Chen, C. H. 1995. Continuous degradation of phenol by Rhodococcus sp. immobilizaed on granular activated carbon and in calcium alginate. Bioresour. Technol. 51, 37-42.   DOI
13 Marrot, B., Barrios-Martinez, A., Moulin, P. and Roche, N. 2006. Biodegradation of high phenol concentration by activated sludge in an immersed membrane bioreactor. Biochem. Eng. J. 30, 174-183.   DOI
14 Masque, C., Nolla, M. and Bordons, A. 1987. Selection and adaptation of a phenol-degrading strain of Pseudomonas. Biotechnol. Lett. 9, 655-660.   DOI
15 Oh, Y. S., Shareefdeen, Z., Baltzis, B. C. and Bartha, R. 1994. Interactions between benzene, toluene, and p-xylene (BTX) during their biodegradation. Biotechnol. Bioeng. 44, 533-538.   DOI
16 Vrijheid, M., Casas, M., Gascon, M., Valvi, D. and Nieuwenhuijsen, M. 2016. Environmental pollutants and child health-a review of recent concerns. Int. J. Hyg. Environ. Health 219, 331-342.   DOI