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http://dx.doi.org/10.5352/JLS.2020.30.1.40

Anti-inflammatory and Anti-bacterial Activities of Artemisia iwayomogi Kitamura Extract Fractions  

Sin, Seung Mi (Gyeongnam Oriental Anti-aging Institute)
Jeong, Won Min (Gyeongnam Oriental Anti-aging Institute)
Kil, Young Sook (Gyeongnam Oriental Anti-aging Institute)
Lee, Dong Yeol (Gyeongnam Oriental Anti-aging Institute)
Kim, Sang Gon (Gyeongnam Oriental Anti-aging Institute)
Goo, Young-Min (Gyeongnam Oriental Anti-aging Institute)
Publication Information
Journal of Life Science / v.30, no.1, 2020 , pp. 40-44 More about this Journal
Abstract
This study investigated the anti-bacterial and anti-inflammatory activities of Artemisia iwayomogi Kitamura for medical and food additive applications. A 70% methanol extract of A. iwayomogi Kitam. was fractionated with a series of solvents in order of increasing polarity; these fractionated extracts showed high 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) scavenging activity in the ethyl acetate fractions and high 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity in both the ethyl acetate and chloroform fractions. Levels of total phenolics and flavonoids were highest in the ethyl acetate fraction extracts. Eight bacteria were then treated with the A. iwayomogi Kitam. extract fractions and most showed similar or lower levels of anti-bacterial activity when compared to the control group, except for Staphylococcus aureus. In addition, the anti-oxidant activities of the ethyl acetate fractions of the A. iwayomogi Kitam. extract were higher than those of the other fractions. To confirm the anti-inflammatory activity of the extract fractions, we applied them to RAW 264.7 cells using LPS, and the results indicate that an ethyl acetate fraction at 250 ㎍/ml concentration inhibits nitric oxide production. This study demonstrates that an ethyl acetate fraction of A. iwayomogi Kitam. extract inhibits bacterial and inflammatory activities and could be useful as a potential source of bioactive compounds.
Keywords
Antibacterial activities; anti-inflammatory; Artemisia iwayomogi Kitam; fraction;
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1 Jo, C. H., Kim, S. Y. and An, I. S. 2014. The improving effect of Centella asiatica extracts on erythema on scalp of aged 20-50's women. Kor. J. Aesthet Cosmetol. 12, 921-927.
2 Kim, O. T., Kim, M. Y., Kim, S. J., Kim, Y. J., Kim, K. S., Ahn, J. C., Kim, S. W. and Hwang, B. 2002. Seasonal variations of triterpene glycosides contents in the leaf of Artemisia gmelinii Weber ex Stechm(L.) Urban. J. Kor. Med. Crop Sci. 10, 375-378.
3 Lee, S. D., Park, H. H., Kim, D. W. and Bang, B. H. 2000. Bioactivie consituents and utilities of Artemisia sp. as medicinal herb and foodstuff. Kor. J. Food Nutr. 13, 490-505.
4 Mabry, T., Markham, K. R. and Tomas, M. B. 1970. The systematic identification of flavonoids. J. Mol. Struct. 10, 320.   DOI
5 Ministry of Food and Drug Safety. 2007.The Korean Herbal Pharmacopoeia. Shinilbooks, seoul, 285-371.
6 Morrissey, P. A. and O'Brien, N. M. 1998. Dietary antioxidants in health and disease. Int. Diary J. 8, 463-472.   DOI
7 Nam, S. M., Kim, J. G., Han, S. S., Kim, S. J., Chung, M. E. and Chung, C. K. 1999. Effects of Artemisia iwayomogi KITAMURA ethanol extracts on lowering serum and liver lipids in rats. J. Kor. Soc. Food Sci. Nutr. 28, 199.
8 Padda, M. S. and Picha, D. H. 2008. Effect of low temperature storage on phenolic composition and antioxidant activity of sweetpotatoes. Postharvest Biol. Tech. 47, 176-180.   DOI
9 Jung, M. J., Yin, Y., Heo, S. I. and Wang, M. H. 2008. Antioxidant and anticancer activities of extract from artemisia capillaries. Kor. J. Pharmacogn. 39, 194-198.
10 Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26, 1231-1237.   DOI
11 Seo, K. S. and Yun, K. W. 2008 Antioxidant Activities of Extracts from Artemisia capillaris THUNB. and Artemisia iwayomogi KITAM. used as Injin. Kor. J. Plant Res. 21, 292-298.
12 Seo, K. S. and Yun, K. W. 2011. Antimicrobial activity and total polyphenol content of extracts from Artemisia capillaris Thunb. and Artemisia iwayomogi Kitam. Used as Injin. Kor. J. Plant Res. 24, 10-16.   DOI
13 Wang, X. J., Sun, H. and Liu, Z. S. 1994. Quantitative analysisof 6,7-dimethylesculetin and capillarisine in Artemisia capillaries Thunb. and prescriptions containing the crude drug. Zhongguo Zhong Yao Za Zhi 19, 667-670.
14 Floegel, A., Kim, D. O., Chung, S. J., Koo, S. I. and Chun, O. K. 2011. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J. Food Com. Anal. 24, 1041-1048.
15 Choi, S. R., You, D. H., Kim, J. Y., Park, C. B., Ryu, J., Kim, D. H. and Eun, J. S. 2008. Antioxidant and antimicrobial activities of Artemisia capillaris Thunbergii. Kor. J. Med. Crop Sci. 16, 112-117.
16 Choi, Y. M., Chung, B. H., Lee, J. S. and Cho, Y. G. 2006. The antioxidant t activities of Artemisia spp. collections. Kor. J. Crop Sci. 51, 209-214.