Browse > Article
http://dx.doi.org/10.5352/JLS.2020.30.12.1085

Analysis of Physiological Activity and Cytotoxicity of Residue Extracts after Pepper Harvest  

Kim, Tae-Won (Institute of Agriculture and Life Science, Gyeongsang National University)
Jeon, Byeong-Gyun (Department of Biology Education, Gyeongsang National University)
Lee, Sung-Ho (Division of Life Science, Gyeongsang National University)
Publication Information
Journal of Life Science / v.30, no.12, 2020 , pp. 1085-1091 More about this Journal
Abstract
The purpose of this study was to investigate the value of residue discarded after harvesting a species of pepper (Capsicum annuum L.). This study analyzed the physiological activity and cytotoxicity of a fermented extract and a hot water extract gathered from the residues of the Cheongyang pepper and the Nokkwang pepper. The total nitrogen, phosphoric acid, potassium, calcium, and magnesium contents of the fermented extract of the Nokkwang pepper were approximately twice as high as those of the Cheongyang pepper, while the hot water extracts had similar levels of these components across both peppers. Among the microelements, only boric acid, iron, and silicon components were detected, whereas zinc, manganese, molybdenum, and copper components were not detected in either extract. The total polyphenol and flavonoid contents of the fermented extracts were more than two times higher than those of the hot water extracts for both peppers. The DPPH radical scavenging ability (indicating antioxidant activity) of the fermented extract was higher than that of the hot water extract, while the ABTS radical scavenging ability (indicating antioxidant activity) of the hot water extract was higher than that of the fermented extract. A cytotoxicity test was conducted on the extracts using an MTT assay. Both extracts exhibited weak cytotoxicity at all concentrations for both peppers. These results suggest that the extract of residue discarded after harvesting these peppers could be suitable for functional feed or agricultural materials.
Keywords
Cytotoxicity; extract; pepper residues; physiological activity;
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
연도 인용수 순위
1 An, N. H., Jo, J. R., Kim, Y. K., Lee, Y., Jee, H. J., Lee, S. M., Park, K. L. and Lee, B. M. 2012. The survey of actual using conditions of farm-made liquid fertilizers for cultivation environment-friendly agricultural products. Kor. J. Organic Agri. 20, 345-356.
2 Appel, H. M., Govenor, H. L., D'Ascenzo, M., Siska, E. and Schultz, J. C. 2001. Limitation of folin assays of folia phenoics in ecological studies. J. Chem. Ecol. 27, 761-778.   DOI
3 Brand-Williams, W., Cuvelier, M. E. and Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol. 28, 25-30.   DOI
4 Cho, Y. S. 2015. Effect of application amount of liquid fertilizer produced from tomato residue on the fruit growth and composition of organic tomatoes. MS dissertation, Chungnam National University, Daejeon, Korea.
5 Cho, Y. S., Cho, M. C. and Suh, H. D. 2000. Current status and projects of national hot pepper industry in Korea. J. Kor. Capsicum Res. Coop. 6, 1-27.
6 Choi, S. R., Kim, M. J., Ahn, M. S., Song, E. J., Seo, S. Y., Choi, M. K., Kim, Y. S., Choi, D. G. and Song, Y. J. 2014. Cytotoxicity of extracts from Korean pepper (Capsicum annuum L.) by extraction solvents and plant parts. Kor. J. Med. Crop Sci. 22, 369-377.   DOI
7 Hong, S., Han S. and Jang, H. 2020. Analysis on the inhibitory effects of frozen pepper imports from China by tariff-rate quota, a trade policy tool using a structural equation model. Kor. J. of Agricultural Sci. 47, 337-347.   DOI
8 Lee, S. E., Ham, H. M., Kim, Y. H., Sung, J. H., Hwang, I. G., Yu, S. M., Jeong, H. S. and Lee, J. S. 2013. The content of capsaicinoids in peppers by cultivation region in Korea. J. Kor. Soc. Food Sci. Nutr. 42, 129-133.   DOI
9 Han, J. H., Lee, Y. S. and Song, K. H. 2019. An analysis on consumers' perceptions about consumption of premium chili powder. Kor. J. Agricultural Economics 60, 67-83.
10 Higa, T. 2001. Effective microorganisms in the context of Kyusei Nature Farming: a technology for the future. In: Senanayake YDA, Sangakkara UR(Eds.), Sixth International conference on Kyusei Nature Farming. pp. 40-43. Pretoria, South Africa.
11 Huang, Y., So, Y. J., Hwang, J. R., Yoo, K. M., Lee, K. W., Lee, Y. J. and Hwang, I. K. 2014. Comparative studies on phytochemicals and bioactive activities in 24 new varieties of red pepper. Kor. J. Food Sci. Technol. 46, 395-403.   DOI
12 Jeon, G., Han, J., Choi, Y., Lee, S. M., Kim, H. T. and Lee, J. 2008. Antioxidant and antiproliferative activity of pepper (Capsicum annuum L.) leaves. J. Kor. Soc. Food Sci. Nutr. 37, 1079-1083.   DOI
13 Jeong, C. H., Ko, W. H., Cho, J. R., Ahn, C. G. and Shim, K. H. 2006. Chemical components of Korean paprika according to cultivars. Kor. J. Food Preserv. 13, 43-49.
14 Jia, Z., Tang, M. and Wu, J. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64, 555-559.   DOI
15 Kim, C. H., Lee, M. A., Kim, T. W., Jang, J. Y. and Kim, H. J. 2012. Anti-inflammatory effect ao Allium hookeri root methanol extract in LPS-induced RAW264.7 cells. J. Kor. Soc. Food Sci. Nutr. 41, 1645-1648.   DOI
16 Jung, M., Hwang, Y., Kim, H. Y., Cho, M. C., Hwang I. G., Yoo, M. Y., Jeong, H. S. and Lee, J. 2011. Evaluation of biological activity in pepper (Capsicum annuum L.) breeding lines. J. Kor. Soc. Food Sci. Nutr. 40, 642-648.   DOI
17 Kim, D. Y., Choi, M. A., Han, Y. H. and Park, S. K. 2016. A study on estimation of air pollutants emission from agricultural waste burning. J. Kor. Soc. Atmos. Environ. 32, 167-175.   DOI
18 Kim, S., Kim, K. S. and Park, J. B. 2006. Changes of various chemical components by the difference of the degree of ripening and harvesting factors in two single-harvested peppers (Capsicum annuum L.). Kor. J. Food Sci. Technol. 38, 615-620.
19 Kim, T. W., Lee, G. H., Jeon, B. G. and Lee, S. H. 2018. Analysis of physiological activity and cytotoxicity of fermented and hot water extracts using residues after onion harvest. J. Life Sci. 28. 1163-1169.   DOI
20 Kim, T. W., Lee, G. H., Jeon, B. G. and Lee, S. H. 2019. Analysis of physiological activity and cytotoxicity of fermented and hot water extracts using residues after sweet potato (Ipomoea batatas L.) harvest. J. Agriculture Life Sci. 53, 93-103.
21 Kim, S., Min, J. and Kim, H. T. 2019. Mycological characteristics and field fitness of Colletotrichum acutatum resistant to pyraclostrobin. Kor. J. Pestic. Sci. 23, 231-239.   DOI
22 Kim, N., Seo, D., Cheon, W., Choi, Y., Lee, J. and Kim, Y. 2020. Bioactive nutritional compounds and antioxidant activity of green peppers consumed in Korea. J. Kor. Soc. Food Sci. Nutr. 49, 141-148.   DOI
23 Lee, Y. R. 2017. Anti-oxidative and anti-proliferative effect of 70% ethnol extracts from green pepper (Capsicum annuum L. cv. DangZo). Kor. J. Food Nutr. 30, 1127-1131.   DOI
24 Park, J. B., Lee, S. M. and Kim, S. 2000. Capsaicinoids control of red pepper powder by particle size. J. Kor. Capsicum Res. Coop. 6, 51-62.
25 Lee, M. W., Park, J. E., Jang, E. J., Son, H. J., Park, H. C., Hong, C. O., Lee, S. B., Shim, C. K., Ko, B. G. and Kim, K. K. 2017. Comparative analysis of functional components of organic and conventional cultivated fruit vegetables commercially distributed in Korea. J. Life Sci. 27, 1176-1184.   DOI
26 Materska, M., Piacente, S., Stochmal, A., Pizza, C., Olezek, W. and Peruka, I. 2003. Isolation and structure elucidation of flavonoid and phenolic acid glycosides from pericarp of hot pepper fruit Capsicum annuum L. Phytochemistry 63, 893-898.   DOI
27 Park, S. K., Hong, Y. S., Kim, D., Kim, D. Y. and Jang, Y. K. 2015. Emission of air pollutants from agricultural crop residues burning. J. KOSAE 31, 63-71.   DOI
28 Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26, 1231-1237.   DOI
29 Wang, B. S., Chen, J. H., Liang, Y. C. and Duh, P. D. 2005. Effects of welsh onion on oxidation of low-density lipoprotein and nitric oxide production in macrophage cell line RAW 264.7. Food Chem. 91, 147-155.   DOI
30 Won, K. Y. and Oh, K. K. 2009. Optimization the xylose fractionation conditions of peeper stem with dilute sulfuric acid. KSBB J. 24, 361-366.
31 Yi, T. G., Park, Y., Choi, I. Y. and Park, N. I. 2019. Comparison of metabolite levels and antioxidant activity among pepper cultivars. Kor. J. Breed. Sci. 51, 326-340.   DOI
32 Yoon, H. J., Lee, S. and Hwang, I. K. 2012. Effects of green pepper (Capsicum annuum var.) on antioxidant activity and induction of apoptosis in human breast cancer cell lines. Kor. J. Food Sci. Technol. 44, 750-758.   DOI
33 Chung, Y. Z. 2003. Induction of cancer cell apoptosis by the extract of Capsicum annuum L. var. angulosum Mill sorted according to the parts in hepatoma cells and MCF-7 cells. J. Pharm. Soc. Kor. 47, 57-68.
34 Davis, B. H., Mathews, S. and Kirk, J. T. O. 1970. The nature and biosynthesis of the carotenoids of different colour varieties of Capsicum annuum L. Phytochem. 9, 797-805.   DOI
35 Yoon, J., Jun, J. J., Lim, S. C., Lee, K. H., Kim, H. T., Jeong, H. S. and Lee, J. 2010. Changes in selected components and antioxidant and antiproliferative activity of peppers depending on cultivation. J. Kor. Soc. Food Sci. Nutr. 39, 721-736.