Browse > Article
http://dx.doi.org/10.5352/JLS.2020.30.12.1054

Oxya Chinensis Sinuosa Mishchenko Extract: Potent Glycosidase Inhibitor Alleviates Postprandial Hyperglycemia in Diabetic Mice  

Park, Jae Eun (Department of Food Science and Nutrition, Pusan National University)
Han, Ji Sook (Department of Food Science and Nutrition, Pusan National University)
Publication Information
Journal of Life Science / v.30, no.12, 2020 , pp. 1054-1062 More about this Journal
Abstract
This study was designed to investigate whether extracts from Oxya chinensis sinuosa Mistshenk (an edible insect considered a grasshopper) could inhibit the activity of carbohydrate digestive enzymes and alleviate postprandial hyperglycemia in diabetic mice. Oxya chinensis sinuosa Mistshenk was extracted with 80% ethanol (OEE) or water (OWE) and then concentrated. The carbohydrate digestive enzyme-inhibiting activity of the resulting extracts was evaluated by examining α-glucosidase and α-amylase. The IC50 values of OEE against α-glucosidase and α-amylase were 0.229 mg/ml and 0.106 mg/ml, respectively. This result indicated that OEE has stronger inhibitory effects than OWE and positive control. The blood glucose levels of the diabetic control mice increased after one meal. However, when OEE (300 mg/kg) was added to starch, this increase in postprandial blood glucose levels was significantly suppressed. The area under the curve also significantly decreased following the administration of OEE, which exhibited no cytotoxicity. These results indicate that OEE is more efficacious than OWE and may be used as a carbohydrate digestive enzyme inhibitor, delay carbohydrate digestion and glucose absorption, and thus alleviate postprandial hyperglycemia caused by dietary carbohydrates.
Keywords
${\alpha}$-amylase; ${\alpha}$-glucosidase; grasshopper; Oxya chinensis sinuosa Mishchenko; postprandial hyperglycemia;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Asano, N., Tomioka, E., Kizu, H. and Matsui, K. 1994. Sugars with nitrogen in the ring isolated from the leaves of Morus bombycis. Carbohydr. Res. 253, 235-245.   DOI
2 Avignon, A., Radauceanu, A. and Monnier, L. 1997. Non-fasting plasma glucose is a better marker of diabetic control than fasting plasma glucose in type 2 diabetes. Diabetes Care 20, 1822-1826.   DOI
3 Fujita, H., Yamagami, T. and Ohshima, K. 2001. Efficacy and safety of Touchi extract, α-glucosidase inhibitor derived from fermented soybeans, in non-insulin-dependent diabetic mellitus. J. Nutr. Biochem. 12, 351-356.   DOI
4 Gerich, J. E. 2003. Clinical significance, pathogenesis, and management of postprandial hyperglycemia. Arch. Intern. Med. 163, 1306-1316.   DOI
5 Goldmann, A., Milat, M. L. and Ducrot, P. H. 1990. Tropane derivatives from Calystegia sepium. Phytochemistry 29, 2125-2128.   DOI
6 Grundy, S. M., Benjamin, I. J., Burke, G. L., Chait, A., Eckel, R. H. and Howard, B. V. 1999. Diabetes and cardiovascular disease: a statement for healthcare professionals from the American Heart Association. Circulation 100, 1134-1146.   DOI
7 Haller, H. 1998. The clinical importance of postprandial glucose. Diabetes Res. Clin. Pract. 40, 43-49.   DOI
8 Hanefeld, M. 1998. The role of acarbose in the treatment of non-insulin-dependent diabetes mellitus. J. Diabetes Complications 12, 228-237.   DOI
9 Hanhineva, K., Torronen, R. and Bondia-Pons, I. 2010. Impact of dietary polyphenols on carbohydrate metabolism. Int. J. Mol. Sci. 11, 1365-1402.   DOI
10 Inoue, I., Takahashi, K., Noji, S., Awata, T., Negishi, K. and Kataya-ma, S. 1997. Acarbose controls postprandial hyperproinsulinemia in non-insulin-dependent diabetes mellitus. Diabetes Res. Clin. Pract. 36, 143-151.   DOI
11 Jeong, B. M., Hyun, M. K., Sin, W. Y., Kim, M. R., Shin, H. C., Yoon, C. H. and Jeong, J. C. 2004. Effects of Bombycis corpus on streptozotocin-induced diabetic rats. J. Internal. Kor. Med. 25, 288-297.
12 Kim, J. S., Kwon, C. S. and Son, K. H. 2000. Alpha-glucosidase inhibitory activities of some wild vegetable extracts. J. Food Sci. Nutr. 5, 174-176.
13 Kalita, D., Holm, D. G., LaBarbera, D. V., Petrash, J. M. and Jayanty, S. S. 2018. Inhibition of alpha-glucosidase, alpha-amylase, and aldose reductase by potato polyphenolic compounds. PLoS One 13, 0191025.
14 Kim, D. S., Choi, M. H. and Shin, H. J. 2018. Polyphenol contents and antioxidant activities of domestic bamboo leaves with different extraction solvents. J. Adv. Engin. Technol. 11, 7-13.
15 Kim, H. J., Kang, S. J., Kim, S. G., Kim, J. E., Koo, H. Y., Park, J. H. and Choi, H. C. 2015. Antioxidant activity and antimicrobial activity of the grasshopper, Oxya chinensis sinuosa. J. Seric. Entomol. Sci. 53, 130-134.   DOI
16 Kim, S. Y., Park, J. E. and Han, J. S. 2019. Tenebrio molitor (Mealworm) extract improves insulin sensitivity and alleviates hyperglycemia in C57BL/Ksj-db/db mice. J. Life Sci. 5, 570-579.
17 Kim, T. S., Lee, J. H., Choi, B. D. and Ryu, H. S. 1987. Nutritional value of dried paddy grasshopper, Oxya chinensis formosana. J. Kor. Soc. Food Nutr. 16, 98-104.
18 Laakso, M. 1999. Hyperglycemia and cardiovascular disease in type 2 diabetes. Diabetes 48, 937-942.   DOI
19 Kim, Y. S. and Kwon, T. D. 2018. Effects of grasshopper diet and treadmill exercise performance of blood lipid profile and antioxidant enzyme in rats. Kor. J. Sports Sci. 27, 1605-1614.   DOI
20 Krentz, A. J. and Bailey, C. J. 2005. Oral antidiabetic agents: current role in type 2 diabetes mellitus. Drugs 65, 385-411.   DOI
21 Li, Y., Wen, S., Kota, B. P., Peng, G., Li, G. Q., Yamahara, J. and Roufogalis, B. D. 2005. Punica granatum flower extract, a potent alpha-glucosidase inhibitor, improves postprandial hyperglycemia in Zucker diabetic fatty rats. J. Ethnopharmacol. 99, 239-244.   DOI
22 Salimifar, M., Fatehi-Hassanabad, Z. and Fatehi, M. 2013. A review of natural products for controlling type 2 diabetes with an emphasis on their mechanisms of action. Curr. Diabetes Rev. 9, 402-411.   DOI
23 Madar, Z. 1984. Effect of brown rice and soybean dietary fiber on the control of glucose and lipid metabolism in rats. Am. J. Clin. Nutr. 43, 388-396.   DOI
24 Narita, Y. and Inouye, K. 2009. Kinetic analysis and mechanism on the inhibition of chlorogenic acid and its components against porcine pancreas α-amylase isozymes I and II. J. Agric. Food Chem. 57, 9218-9225.   DOI
25 Paek, M. K., Hwang, J. M., Jung, K. S., Kim, T. W., Kim, M. C., Lee, Y. J., Cho, Y. B., Park, S. W., Lee, H. S., Ku, D. S., Jeong, J. C., Kim, K. G., Choi, D. S., Shin, E. H., Jwang, J. H., Lee, J. S., Kim, S. S. and Bea, Y. S. 2010. Checklist of Korean Insects. Nature and Ecology. Pub. Seoul, Korea. 36.
26 Park, J. Y, Heo, J. C., Woo, S. U., Yun, C. Y., Kang, S. W., Hwang, J. S. and Lee, S. H. 2006. Anti-inflammatory and cellular protective effects on hydrogen peroxide induced cytotoxicity of grasshopper extracts. Kor. J. Food Preserv. 13, 796-802.
27 Ryu, K. S., Lee, H. S., Kim, K. Y., Kim, M. J., Kang, P. D. and Chun, S. N. 2012. Anti-diabetic effects of the silkworm (Bombyx mori) extracts in the db/db mice. Planta Med. 78, 458.
28 Taek, C. H., Sang, S. K., Yeona, K., Han, C. M., Taewan, K., Hwan, L. S., Lee, D. H. and Ho, K. J. 2019. Anti-diabetic activity of edible insect Gryllus bimaculatus extracts in insulin-deficient diabetic mice. J. Kor. Soc. Food Sci. Nutr. 10, 1165-1171.
29 Tai, E., Lim, S. C., Tan, B. Y., Chew, S. K., Heng, D. and Tan, C. E. 2000. Screening for diabetes mellitus-a two-step approach in individuals with impaired fasting glucose improves detection of those at risk of complications. Diabet. Med. 17, 771-775.   DOI
30 Watanabe, J., Kawabata, J., Kurihara, H. and Niki, R. 1997. Isolation and identification of alpha-glucosidase inhibitors from tochucha (Eucommia ulmoides). Biosci. Biotechnol. Biochem. 61, 177-178.   DOI
31 Yoon, Y. I., Chung, M. Y., Hwang, J. S., Goo, T. W., Ahn, M. Y., Lee, Y. B., Han, M. S. and Yun, E. Y. 2014. Anti-inflammatory effect of Oxya chinensis sinuosa ethanol extract in LPS-induced RAW 264.7 cells. J. Life Sci. 24, 370-376.   DOI
32 Young, I. R. and Stout, R. W. 1987. Effects of insulin and glucose on the cells of the arterial wall: Interaction of insulin with dibutyryl cyclic AMP and low density lipoprotein in arterial cells. Diabete Metab. 13, 301-306.
33 Adisakwattana, S., Chantarasinlapin, P., Thammarat, H. and Yibchok-Anun, S. 2009. A series of cinnamic acid derivatives and their inhibitory activity on intestinal alpha-glucosidase. J. Enzyme Inhib. Med. Chem. 24, 1194-1200.   DOI
34 Abbas, M., Saeed, F., Anjum, F. M., Afzaal, M., Tufail, M. T., Bashir, M. S., Ishtiaq, A., Hussain, S. and Suleria, H. A. 2016. Natural polyphenols: an overview. Int. J. Food Prop. 20, 1689-1699.
35 Abid, S., Lekchiri, A., Mekhfi, H., Ziyyat, A., Legssyer, A., Aziz, M. and Bnouham, M. 2014. Inhibition of α-glucosidase and glucose intestinal absorption by Thymelaea hirsuta fractions. J. Diabetes 6, 351-359.   DOI
36 Adisakwattana, S., Sookkongwaree, K., Roengsumran, S., Petsom, A., Ngamrojnavanich, N., Chavasiri, W., Deesamer, S. and Yibchok-anun, S. 2004. Structure-activity relationships of trans-cinnamic acid derivatives on alpha-glucosidase inhibition. Bioorg. Med. Chem. Lett. 14, 2893-2896.   DOI
37 Adisakwattana, S. 2017. Cinnamic acid and its derivatives: mechanisms for prevention and management of diabetes and its complications. Nutrients 9, 163.   DOI
38 Basu, S., Yudkin, J. S., Kehlenbrink, S., Davies, J. I., Wild, S. H., Lipska, K. J., Sussman, J. B. and Beran, D. 2018. Estimation of global insulin use for type 2 diabetes, 2018-30: a microsimulation analysis. Lancet Diabetes Endocrinol. 7, 25-33.
39 Forman, L. J., Estilow, S., Lewis, M. and Vasilenko, P. 1986, Streptozotocin diabetes alters immunoreactive beta-endorphin levels and pain perception after 8 wk in female rats. Diabetes 35, 1309-1313.   DOI
40 Baron, A. D. Postprandial hyperglycaemia and α-glucosidase inhibitors. 1998. Diabetes Res. Clin. Pract. 40, 51-55.   DOI
41 Bonora, E. and Muggeo, M. 2001. Postprandial blood glucose as a risk factor for cardiovascular disease in type II diabetes; the epidemiological evidence. Diabetologia 44, 2107-2114.   DOI
42 Buszewska-Forajta, M., Struck-Lewicka, W., Bujak, R., Siluk, D. and Kaliszan, R. 2014. Determination of water-soluble components of abdominal secretion of grasshopper (Chorthippus spp.) by GC/MS/MS in search for potential wound healing agents. Chromatographia 77, 1091-1102.   DOI
43 Chinenye, S. and Young, E. E. 2012. Isolated postprandial hyperglycemia in type 2 diabetic patients in a Nigerian Tertiary Health Center. Indian J. Endocrinol. Metab. 16, 604-608.   DOI
44 Chung, M. Y., Kwon, E. Y., Hwang, J. S., Goo, T. W. and Yun, E. Y. 2013. Pretreatment conditions on the powder of Tenbrio molitor for using as a novel food ingredient. J. Seric. Entomol. Sci. 51, 9-14.   DOI
45 Clissold, S. P. and Edwards, C. 1988. Acarbose: a preliminary review of its pharmacodynamic and pharmacokinetic properties, and therapeutic potential. Drugs 3, 214-243.   DOI