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http://dx.doi.org/10.5352/JLS.2020.30.11.965

Physiological Activity of Methanol Extracts from Sambucus sieboldiana var. miquelii (Nakai) Hara  

Oh, Yu Jin (Wild Plant Industrialization Research Division, Baekdudaegan National Arboretum)
Cho, Hae Jin (Wild Plant Industrialization Research Division, Baekdudaegan National Arboretum)
Woo, Hyun Sim (Wild Plant Industrialization Research Division, Baekdudaegan National Arboretum)
Byeon, Jun-Gi (Forest Bioresource Conservation Division, Baekdudaegan National Arboretum)
Kim, Yeong-Su (Wild Plant Industrialization Research Division, Baekdudaegan National Arboretum)
Kim, Dae Wook (Wild Plant Industrialization Research Division, Baekdudaegan National Arboretum)
Publication Information
Journal of Life Science / v.30, no.11, 2020 , pp. 965-972 More about this Journal
Abstract
Sambucus sieboldiana var. miquelii (Nakai) Hara is distributed in Korea, China, and Japan, and has been used as an anti-rheumatic in folk medicine in oriental countries. The present study aims to investigate the potential use of this species in health functional foods, cosmetics, and food preservatives. Methanol extracts of leaves and branches from this plant were prepared to quantitatively analyze the total phenol and flavonoid contents, and to investigate the antioxidative and enzyme inhibitory activities, and the inhibition of nitric oxide (NO) production activity. The results showed that the total polyphenol and flavonoid contents of the crude extract were 1.52±0.1 mg/g and 1.73±0.1 mg/g, respectively. S. sieboldiana polyphenols exhibited potent scavenging activity shown by 2, 2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and 2, 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation assay. The crude extract also exhibited significant α-glucosidase and tyrosinase inhibitory activity with IC50 values of 183.5 ㎍/ml and 323.9 ㎍/ ml, respectively. Additionally, the crude extract exhibited strong anti-inflammatory activity determined through the nitric oxide inhibition assay in a dose-dependent manner with an IC50 value of 36.7 ㎍/ml and no cytotoxic effect on the macrophages. Therefore, we demonstrated that the leaves and branches of S. sieboldiana extract possess antioxidant, anti-diabetic, depigmentation potential, and NO production inhibitory activities. According to recent research, S. sieboldiana has great potential as a source of the bioactive compound which could be used as food, cosmetics, and pharmaceutical agents.
Keywords
${\alpha}$-Glucosidase inhibitory activity; anti-inflammatory effect; antioxidant; Sambucus sieboldiana var. miquelii (Nakai) Hara; tyrosinase inhibitory activity;
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1 Hwang, B., Cho, J., Hwang, I. S., Jin, H. G., Woo, E. R., and Lee, D. G. 2011. Antifungal activity of lariciresinol derived from Sambucus williamsii and their membrane-active mechanisms in Candida albicans. Biochem. Biophys. Res. Commun. 410, 489-493.   DOI
2 Jang, B. C., Paik, J. H., Kim, S. P., Shin, D. H., Song, D. K., Park, J. G. and Suh, S. I. 2005. Catalase induced expression of inflammatory mediators via activation of NF-kappaB, PI3K/AKT, p70S6K, and JNKs in BV2 microglia. Cell. Signal. 17, 625-633.   DOI
3 Jimenez-Cervantes, C., Solano, F., Kobayashi, T., Urabe, K., Hearing, V. J., Lozano, J. A. and Garcia-Borron, J. C. 1994. A new enzymatic function in the melanogenic pathway. The 5, 6-dihydroxyindole-2-carboxylic acid oxidase activity of tyrosinase-related protein-1 (TRP1). J. Biol Chem. 269, 17993-18000.   DOI
4 Abdel-Hameed, El-S. S. 2008. Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples. Food Chem. 114, 1271-1277.   DOI
5 Kim, K. Y., Nam, K. A., Kurihara, H. and Kim, S. M. 2008. Potent α-glucosidase inhibitors purified from the red alga Grateloupia elliptica. Phytochemistry 69, 2820-2825.   DOI
6 Joung, Y. M., Park, S. J., Lee, K. Y., Lee, J. Y., Suh, J. K., Hwang, S. Y., Park, K. E. and Kang, M. H. 2007. Antioxidative and antimicrobial activities of Lilium species extracts prepared from different aerial parts. Kor. J. Food Sci Technol. 39, 452-457
7 Kalyanaraman, B. 2013. Teaching the basics of redox biology to medical and graduate students: oxidants, antioxidants and disease mechanisms. Redox. Biol. 1, 244-257.   DOI
8 Kedare, S. B. and Singh, R. P. 2011. Genesis and development of DPPH method of antioxidant assay. J. Food Sci. Techol. 48, 412-422.   DOI
9 Kim, Y. S., Lee, S. J., Hwang, J. W., Kim, E. H., Park, P. J. and Jeong, J. H. 2012. Anti-inflammatory effects of extracts from Ligustrum ovalifolium H. leaves on RAW264. 7 macrophages. J. Kor. Soc. Food Sci. Nutr. 41, 1205-1210.   DOI
10 Lee, C. B. 2006. Korea illustrated plant book II. Hyangmoonsa 227, 228.
11 Cencioni, C., Spallotta, F., Martelli, F., Valente, S., Mai, A., Zeiher, A. M. and Gaetano, C. 2013. Oxidative stress and epigenetic regulation in ageing and age-related diseases. Int. J. Mol. Sci. 14, 17643-17663.   DOI
12 Choi, H., Lee, J., Chang, Y. S., Woo, E. R. and Lee, D. G. 2013. Isolation of (-)-olivil-9'-O-β-d-glucopyranoside from Sambucus williamsii and its antifungal effects with membrane-disruptive action. Biochim. Biophys. Acta 1828, 2002-2006.   DOI
13 Clissold, S. P. and Edwards, C. 1988. Acarbose. Drugs 35, 214-243.   DOI
14 Dabhi, A. S., Bhatt, N. R. and Shah, M. J. 2013. Voglibose: an alpha glucosidase inhibitor. JCDR 7, 3023.
15 de Melo, C. L., Queiroz, M. G. R., Arruda Filho, A. C. V., Rodrigues, A. M., de Sousa, D. F., Almeida, J. G. L. and Santos, F. A., et al. 2009. Betulinic acid, a natural pentacyclic triterpenoid, prevents abdominal fat accumulation in mice fed a high-fat diet. J. Agric. Food Chem. 57, 8776-8781.   DOI
16 Gonzalez-Burgos, E. and Gomez-Serranillos, M. P. 2012. Terpene compounds in nature: a review of their potential antioxidant activity. Curr. Med. Chem. 19, 5319-5341.   DOI
17 Lee, J. Y., Yoo, D. H., Jeng, Y. S., Joo, S. H. and Chae, J. W. 2018. Verification of anti-inflammatory activities of the ethanol extracts of Glechoma hederacea var. longituba in RAW 264.7 cells. J. Life Sci. 28, 429-434.   DOI
18 Ahn, J., Gammon, M. D., Santella, R. M., Gaudet, M. M., Britton, J. A., Teitelbaum, S. L., Terry, M. B., Nowell, S., Davis, W., Garza, C., Neugut, A. l. and Neugut, A. I. 2005. Associations between breast cancer risk and the catalase genotype, fruit and vegetable consumption, and supplement use. Am. J. Epidemiol. 162, 943-952.   DOI
19 Berneburg, M., Plettenberg, H. and Krutmann, J. 2000. Photoaging of human skin. Photodermatol. Photoimmunol. Photomed. 16, 239-244.   DOI
20 Chae, J. W. and Cho, Y. J. 2012. Antioxidative Activity of Extracts from Sambucus williamsii var. coreana. Kor. J. Plant Res. 25, 363-371.   DOI
21 Lima-Junior, R. C. P., Sousa, D. I. M., Brito, G. A. C., Cunha, G. M., Chaves, M. H., Rao, V. S. N. and Santos, F. A. 2007. Modulation of acute visceral nociception and bladder inflammation by plant triterpene, α, β-amyrin in a mouse model of cystitis: role of tachykinin NK 1-receptors, and K+ ATP channels. Inflammation Res. 56, 487-494.   DOI
22 Lee, K. H., Nam, H. O. and Yoon, W. H. 2007. Effect of protein-bond polysaccharide isolated from Acanthopanax senthopanax in reducing the toxic effect of cisplatin. Kor. J. Pharmacogn. 38, 1-17.
23 Lee, Y. M., Bae, J. H., Kim, J. B., Kim, S. Y., Chung, M. N., Park, M. Y., Ko, J. S., Song, J. and Kim, J. H. 2012. Changes in the physiological activities of four sweet potato varieties by cooking condition. Kor. J. Nutr. 45, 12-19.   DOI
24 Lerner, A. B. and Fitzpatrick, T. B. 1950. Biochemistry of melanin formation. Physiol. Rev. 30, 91-126.   DOI
25 Loizzo, M. R., Bonesi, M., Di Lecce, G., Boselli, E., Tundis, R., Pugliese, A., Menichini, F. and Frega, N. G. 2013. Phenolics, aroma profile, and in vitro antioxidant activity of Italian dessert passito wine from Saracena (Italy). J. Food Sci. 78, C703-708.   DOI
26 Lowenstein, C. J., and Snyder, S. H. 1992. Nitric oxide, a novel biologic messenger. Cell 70, 705-707.   DOI
27 Lv, H., Chen, S., Xu, X., Zhu, M., Zhao, W., Liu, K. and Liu, K. 2015. Isolation of linoleic acid from Sambucus williamsii seed oil extracted by high pressure fluid and its antioxidant, antiglycemic, hypolipidemic activities. Int. J. Food Engineer. 11, 383-391.   DOI
28 VJ, H. 1987. Mammalin monophenol monoxygenase (tyrosinase): Purification, properties and reaction catalyzed. Methods Enzymol. 142, 154-165   DOI
29 Nathan, C. and Xie, Q. W. 1994. Nitric oxide synthases: roles, tolls, and controls. Cell 78, 915-918.   DOI
30 Manach, C., Scalbert, A., Morand, C., Remesy, C. and Jimenez, L. 2004. Polyphenols: food sources and bioavailability. Am. J. Clin. Nutr. 79, 727-747.   DOI
31 Oliveira, F. A., Chaves, M. H., Almeida, F. R., Lima Jr, R. C. P., Silva, R. M., Maia, J. L., Brito, G. A. and Rao, V. S. 2005. Protective effect of α-and β-amyrin, a triterpene mixture from Protium heptaphyllum (Aubl.) March. trunk wood resin, against acetaminophen-induced liver injury in mice. J. Ethnopharmacol. 98, 103-108.   DOI
32 Park, H. Y., Kosmadaki, M., Yaar, M. and Gilchrest, B. A. 2009. Cellular mechanisms regulating human melanogenesis. Cell Mol. Life Sci. 66, 1493-1506.   DOI
33 Pillai, S., Oresajo, C. and Hayward, J. 2005. Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation‐induced matrix degradation-a review. Int. J. Cosmet. Sci. 27, 17-34.   DOI
34 Radi, R., Beckman, J. S., Bush, K. M. and Freeman, B. A. 1991. Peroxynitrite oxidation of sulfhydryls. The cytotoxic potential of superoxide and nitric oxide. J. Biol. Chem. 266, 4244-4250.   DOI
35 Shinde, J., Taldone, T., Barletta, M., Kunaparaju, N., Hu, B., Kumar, S., Placido, J. and William, Z. S. 2008. α-Glucosidase inhibitory activity of Syaygium cumini (Linn.) Skeels seed kernel in vitro and in Goto-Kakizaki (GK) rats. Carbohydr Res. 343, 1278-1281.   DOI
36 Sim, H, J., Seo, W. T., Choi, M. H., Kim, K. H., Shin, J. H. and Kang, M. J. 2016. Quality characteristics of vinegar added with different levels of black garlic. Kor. J. Food Cook Sci. 32, 16-26.   DOI
37 Stanely Mainzen Prince, P. and Menon, V. P. 2001. Antioxidant action of Tinospora cordifolia root extract in alloxan diabetic rats. Phytother. Res. 15, 213-218.   DOI
38 Xiao, H. H., Dai, Y., Wong, M. S. and Yao, X. S. 2014. New lignans from the bioactive fraction of Sambucus williamsii Hance and proliferation activities on osteoblastic-like UMR 106 cells. Fitoterapi 94, 29-35.   DOI
39 Suh, W. S., Kim, C. S., Subedi, L., Kim, S. Y., Choi, S. U., and Lee, K. R. 2017. Iridoid Glycosides from the Twigs of Sambucus williamsii var. coreana and Their biological activities. J. Nat. Prod. 80, 2502-2508.   DOI
40 Wang, Z. Y., Han, H., Yang, B. Y., Xia, Y. G. and Kuang, H. X. 2011. Two new iridoid glycosides from the root barks of Sambucus williamsii Hance. Molecules 16, 3869-3874.   DOI
41 Xiao, H. H., Zhang, Y., Cooper, R., Yao, X. S. and Wong, M. S. 2016. Phytochemicals and potential health effects of Sambucus williamsii Hance (Jiegumu). Chin. Med. 11, 36.   DOI