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http://dx.doi.org/10.5352/JLS.2020.30.11.947

Cis-acting Replication Element Variation of the Foot-and-mouth Disease Virus is Associated with the Determination of Host Susceptibility  

Kang, Hyo Rin (Department of Molecular Biology, Pusan National University)
Seong, Mi So (Department of Molecular Biology, Pusan National University)
Ku, Bok Kyung (Animal and Plant Quarantine Agency)
Cheong, JaeHun (Department of Molecular Biology, Pusan National University)
Publication Information
Journal of Life Science / v.30, no.11, 2020 , pp. 947-955 More about this Journal
Abstract
The foot-and-mouth disease virus (FMDV), a member of the Aphthovirus genus in the Picornaviridae family, affects wild and domesticated ruminants and pigs. During replication of the FMDV RNA (ribonucleic acid) genome, FMDV-encoding RNA polymerase 3D acts in a highly location-specific manner. This suggests that specific RNA structures recognized by 3D polymerase within non-coding regions of the FMDV genome assist with binding during replication. One such region is the cis-acting replication element (CRE), which functions as a template for RNA replication. The FMDV CRE adopts a stem-loop conformation with an extended duplex stem, supporting a novel 15-17 nucleotide loop that derives stability from base-stacking interactions, with the exact RNA nucleotide sequence of the CRE producing different RNA secondary structures. Here, we show that CRE sequences of FMDVs isolated in Korea from 2010 to 2017 exhibit A and O genotypes. Interestingly, variations in the RNA secondary structure of the Korean FMDVs are consistent with the phylogenetic relationships between these viruses and reveal the specificity of FMDV infections for particular host species. Therefore, we conclude that each genetic clade of Korean FMDV is characterized by a unique functional CRE and that the evolutionary success of new genetic lineages may be associated with the invention of a novel CRE motif. Therefore, we propose that the specific RNA structure of a CRE is an additional criterion for FMDV classification dependent on the host species. These findings will help correctly analyze CRE sequences and indicate the specificity of host species for future FMDV epidemics.
Keywords
CRE; FMDV; host susceptibility; RNA structure; virus variation;
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1 Alexandersen, S., Zhang, Z., Donaldson, A. I. and Garland, A. 2003. The pathogenesis and diagnosis of foot-and-mouth disease. J. Comp. Pathol. 129, 1-36.   DOI
2 Arzt, J., Juleff, N., Zhang, Z. and Rodriguez, L. 2011. The pathogenesis of foot and mouth disease I: viral pathways in cattle. Transbound. Emerg. Dis. 58, 291-304.   DOI
3 Arzt, J., Baxt, B., Grubman, M., Jackson, T., Juleff, N., Rhyan, J., Rieder, E., Waters, R. and Rodriguez, L. 2011. The pathogenesis of foot and mouth disease II: Viral pathways in swine, small ruminants, and wildlife; myotropism, chronic syndromes, and molecular virus-host interactions. Transbound. Emerg. Dis. 58, 305-326.   DOI
4 Carrillo, C., Lu, Z., Borca, M. V., Vagnozzi, A., Kutish, G. F. and Rock, D. L. 2007. Genetic and phenotypic variation of foot-and-mouth disease virus during serial passages in a natural host. J. Virol. 81, 11341-11351.   DOI
5 Carrillo, C., Tulman, E. R., Delhon, G., Lu, Z., Carreno, A., Vagnozzi, A., Kutish, G. F. and Rock, D. L. 2005. Comparative genomics of foot-and-mouth disease virus. J. Virol. 79, 6487-6504.   DOI
6 de Borba, L., Villordo, S. M., Iglesias, N. G., Filomatori, C. V., Gebhard, L. G. and Gamarnik, A. V. 2015. Overlapping local and long-range RNA-RNA interactions modulate dengue virus genome cyclization and replication. J. Virol. 89, 3430-3437.   DOI
7 Ferrer-Orta, C., Agudo, R., Domingo, E. and Verdaguer, N. 2009. Structural insights into replication initiation and elongation processes by the FMDV RNA-dependent RNA polymerase. Curr. Opin. Struct. Biol. 19, 752-758.   DOI
8 Gao, Y., Sun, S. and Guo, H. 2016. Biological function of Foot-and-mouth disease virus non-structural proteins and non-coding elements. Virol. J. 13, 1-17.   DOI
9 Herod, M. R., Ferrer-Orta, C., Loundras, E. A., Ward, J. C., Verdaguer, N., Rowlands, D. J. and Stonehouse, N. J. 2016. Both cis and trans activities of foot-and-mouth disease virus 3D polymerase are essential for viral RNA replication. J. Virol. 90, 6864-6883.   DOI
10 Gritsun, T. and Gould, E. 2006. Origin and evolution of 3' UTR of flaviviruses: long direct repeats as a basis for the formation of secondary structures and their significance for virus transmission. Adv. Virus Res. 69, 203-248.   DOI
11 Horsington, J. and Zhang, Z. 2007. Consistent change in the B-C loop of VP2 observed in foot-and-mouth disease virus from persistently infected cattle: Implications for association with persistence. Virus Res. 125, 114-118.   DOI
12 Juleff, N., Windsor, M., Lefevre, E. A., Gubbins, S., Hamblin, P., Reid, E., McLaughlin, K., Beverley, P. C., Morrison, I. W. and Charleston, B. 2009. Foot-and-mouth disease virus can induce a specific and rapid CD4+ T-cell-independent neutralizing and isotype class-switched antibody response in naive cattle. J. Virol. 83, 3626-3636.   DOI
13 Knowles, N. and Samuel, A. 2003. Molecular epidemiology of foot-and-mouth disease virus. Virus Res. 91, 65-80.   DOI
14 Liu, Y., Wimmer, E. and Paul, A. V. 2009. Cis-acting RNA elements in human and animal plus-strand RNA viruses. Biochim. Biophys. Acta 1789, 495-517.   DOI
15 Samuel, A. and Knowles, N. 2001. Foot-and-mouth disease type O viruses exhibit genetically and geographically distinct evolutionary lineages (topotypes). J. Gen. Virol. 82, 609-621.   DOI
16 Markoff, L., Pang, X., Houng Hs, H. S., Falgout, B., Olsen, R., Jones, E. and Polo, S. 2002. Derivation and characterization of a dengue type 1 host range-restricted mutant virus that is attenuated and highly immunogenic in monkeys. J. Virol. 76, 3318-3328.   DOI
17 Mason, P. W., Bezborodova, S. V. and Henry, T. M. 2002. Identification and characterization of a cis-acting replication element (cre) adjacent to the internal ribosome entry site of foot-and-mouth disease virus. J. Virol. 76, 9686-9694.   DOI
18 Nayak, A., Goodfellow, I. G., Woolaway, K. E., Birtley, J., Curry, S. and Belsham, G. J. 2006. Role of RNA structure and RNA binding activity of foot-and-mouth disease virus 3C protein in VPg uridylylation and virus replication. J. Virol. 80, 9865-9875.   DOI
19 Paul, A. V. and Wimmer, E. 2015. Initiation of proteinprimed picornavirus RNA synthesis. Virus Res. 206, 12-26.   DOI
20 Salonen, A., Ahola, T. and Kaariainen, L. 2004, "Viral RNA replication in association with cellular membranes" in Membrane trafficking in viral replication Springer, pp. 139-173.
21 Spear, A., Sharma, N. and Flanegan, J. B. 2008. Protein-RNA tethering: The role of poly (C) binding protein 2 in poliovirus RNA replication. Virology 374, 280-291.   DOI
22 Steil, B. P. and Barton, D. J. 2009. Conversion of VPg into VPgpUpUOH before and during poliovirus negative-strand RNA synthesis. J. Virol. 83, 12660-12670.   DOI
23 Stenfeldt, C., Pacheco, J. M., Brito, B. P., Moreno-Torres, K. I., Branan, M. A., Delgado, A. H., Rodriguez, L. L. and Arzt, J. 2016. Transmission of foot-and-mouth disease virus during the incubation period in pigs. Front. Vet. Sci. 3, 105.
24 Villordo, S. M. and Gamarnik, A. V. 2013. Differential RNA sequence requirement for dengue virus replication in mosquito and mammalian cells. J. Virol. 87, 9365-9372.   DOI
25 Villordo, S. M., Filomatori, C. V., Sanchez-Vargas, I., Blair, C. D. and Gamarnik, A. V. 2015. Dengue virus RNA structure specialization facilitates host adaptation. PLoS Pathog. 11, e1004604.   DOI