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http://dx.doi.org/10.5352/JLS.2019.29.9.1027

Osteoblastogenic Activity of Tenebrio molitor Larvae Oil on the MG-63 Osteoblastic Cell  

Seo, Minchul (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Baek, Minhee (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Joon Ha (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Hwa Jeong (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, In-Woo (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Sun Young (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Hwang, Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Mi-Ae (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Publication Information
Journal of Life Science / v.29, no.9, 2019 , pp. 1027-1033 More about this Journal
Abstract
Recently, Korea has seen a rapid increase in the elderly population. As a result, osteoporosis, a geriatric disease, has become a social problem. To investigate the novel and natural materials for promoting osteoblastogenesis, we investigated the osteoblastogenic activity of Tenebrio molitor larvae oil (TMO) on the MG-63 preosteoblast cells. The cytotoxicity and proliferation effects of TMO on MG-63 cells were measured by MTS assay. There was no cytotoxicity up to $80{\mu}g/ml$. At 40 and $80{\mu}g/ml$ of TMO (treated for 48 hr), cell proliferation was elevated about 120% compared to the control. The osteoblastogenic activity of TMO was measured with alkaline phosphatase (ALP) activity at 5 days. Doses of 5 to $80{\mu}g/ml$ of TMO increased ALP activities significantly compared with the control. In addition, expression of ALP and Runx2 (osteoblastogenic markers) were markedly increased after treatment of TMO for 5 days. These results provide evidence that TMO promotes osteoblastogenesis by increasing the gene and protein expression of ALP and Runx2, and they suggest that TMO may be a potential agent for bone formation and preventing osteoporosis.
Keywords
Alkaline phosphatase; bone formation; MG-63; osteoblastogenesis; Tenebrio molitor;
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