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http://dx.doi.org/10.5352/JLS.2019.29.9.1016

Inhibitory Effect of the Ethanol Extract of Torilis Japonica Decandolle on Adipocyte Differentiation in 3T3-L1 Cells  

Nam, Gun He (Department of Biological Science and Biotechnology, Hannam University)
Wee, Ji-Hyang (Department of Food Science & Bio Technology, Shinansan University)
Kim, Sang Yung (Department of Food Science & Bio Technology, Shinansan University)
Baek, Ji-Young (Department of Pharma-Gene Co., Ltd.)
Kim, Young Min (Department of Biological Science and Biotechnology, Hannam University)
Publication Information
Journal of Life Science / v.29, no.9, 2019 , pp. 1016-1022 More about this Journal
Abstract
Owing to increased interest in preventing obesity in an aging society, both men and women spend considerable amount of cost on obesity managements. In this study, we investigated the natural substances on anti-obesity activities in 3T3-L1 pre-adipocytes. Also, to improve anti-obesityeffects, research using 3T3-L1 pre-adipocytes cells is crucial. The anti-obesity effect of 70% ethanol extract from Torilis Japonica Decandolle on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated by suppressing adipocyte differentiation and lipid accumulation with Oil Red O assay, and western blot analysis. Compared to the control, 70% ethanol extract of Torilis Japonica Decandolle was significantly inhibited adipocyte differentiation and intracellular triglyceride (TG) level at a concentration of $100{\mu}g/ml$. To determine the mechanism of reduction in TG content, we determined the level of protein expression of obesity-related proteins, such as peroxisome-proliferatorsactivated-receptor-${\gamma}$ ($PPAR{\gamma}$) and CCAAT enhancer-binding-proteins-${\alpha}$ ($C/EBP{\alpha}$), and Acetyl-CoA carboxylase (ACC) phosphorylation. As a results, 70% ethanol extract of Torilis Japonica Decandolle significantly decreased protein expression of $PPAR{\gamma}$, $C/EBP{\alpha}$ and ACC phosphorylation. These results indicate that 70% ethanol extract of Torilis Japonica Decandolle is the most effective candidate for preventing obesity. However further studies will be needed to identify the active compounds that confer the anti-obesity activity of Torilis Japonica Decandolle.
Keywords
ACC; adipocytes; $C/EBP{\alpha}$; $PPAR{\gamma}$; Torilis Japonica D.;
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