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http://dx.doi.org/10.5352/JLS.2019.29.8.871

Regulation of Wnt/β-catenin Signal Transduction in HT-29 Colon Cancer Cells by a Rhododendron brachycarpum Fraction  

Sim, Bo Ram (Beauty care Technology, Dongnam Health University)
Nam, Young Sun (Department of Beauty Design)
Lee, Ja Bok (L.FOUNDER INC., Business Incubation Seoul National University of Science & Technology)
Publication Information
Journal of Life Science / v.29, no.8, 2019 , pp. 871-878 More about this Journal
Abstract
The aim of this study was to determine the anticancer activities of an 80% methanol extract and various fractions of Rhododendron brachycarpum (RB). The n-hexane fraction of RB showed the highest inhibitory activity (Inhibit concentration $50%=20.2{\pm}1.2{\mu}g/ml$) in HT-29 cells. Colony- and sphereforming abilities were significantly correlated with a decrease in the cell count and size. A TOP/FOP flash reporter assay revealed that the inhibitory activity of the n-hexane fraction of RB ($0.22{\pm}0.02$ fold change) was lower than that of the 80% methanol extract and that of other fractions. The n-hexane and ethyl acetate fractions of RB were predominantly dependent on the expression levels of intracellular ${\beta}-catenin$. Western blotting using $p-GSK3{\beta}$ with only the n-hexane fraction of RB was conducted to examine whether these secondary metabolites reduced ${\beta}-catenin$ degradation. Intracellular ${\beta}-catenin$ regulation resulted in quantitative changes in the nucleus. In summary, these results demonstrate the potential of the n-hexane fraction of RB as a natural anticancer agent.
Keywords
Anti-cancer; ${\beta}-catenin$; $p-GSK3{\beta}$; Rhododendron brachycarpum; Wnt;
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