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http://dx.doi.org/10.5352/JLS.2019.29.7.809

Anti-proliferative Properties of p-Coumaric Acid in SNU-16 Gastric Cancer Cells  

Jang, Mi Gyeong (Department of Biology, Jeju National University)
Ko, Hee Chul (Biotechnology Regional Innovation Center, Jeju National University)
Kim, Se-Jae (Department of Biology, Jeju National University)
Publication Information
Journal of Life Science / v.29, no.7, 2019 , pp. 809-816 More about this Journal
Abstract
The ubiquitous plant metabolite p-coumaric acid (p-CA) has antioxidant and anti-inflammatory properties, but its anti-cancer activity has not been established in gastric cancer cell lines. In this study, we investigated the effects of p-CA on the proliferation and transcriptome profile of SNU16 gastric cancer cells. Treatment with p-CA induced apoptosis of the SNU-16 cells by regulating the expression of pro-apoptotic and anti-apoptotic proteins, such as Bcl-2, poly (ADP-ribose) polymerase (PARP), Bax, procaspase-3, and cleaved-caspase-3. The genes differentially expressed in response to p-CA treatment of the SNU-16 cells were identified by RNA sequencing analysis. Genes regulated by p-CA were involved mainly in the inflammatory response, apoptotic processes, cell cycle, and immune response. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that the phosphatidylinositol-3-kinase-Akt and cancer signaling pathways were altered by p-CA. Protein-protein interaction (PPI) network analysis also revealed that p-CA treatment was correlated with differential expression of genes associated with the inflammatory response and cancer. Collectively, these results suggest that p-CA has potential utility in gastric cancer prevention.
Keywords
Gastric cancer cells; p-coumaric acid; RNA-sequencing; transcriptome;
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