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http://dx.doi.org/10.5352/JLS.2019.29.4.395

Characteristics of Phthalate Esters-exposed Boar Sperm during Boar Semen Storage  

Lee, A-Sung (College of Animal Life Science, Kangwon National University)
Lee, Sang-Hee (Institute of Animal Resources, Kangwon National University)
Lee, Seunghyung (College of Animal Life Science, Kangwon National University)
Yang, Boo-Keun (College of Animal Life Science, Kangwon National University)
Publication Information
Journal of Life Science / v.29, no.4, 2019 , pp. 395-401 More about this Journal
Abstract
Phthalate is a chemical endocrine disrupter and interfere with the action of hormones, estrogens, androgens and thyroid hormones. It also affect cardiovascular, metabolic, immune and reproductive system in the human and animals. Curcumin is antioxidant, anti-inflammatory activity and -cancer properties in the human. We studied whether phthalates damage viability, mitochondrial activity and membrane integrity of sperm in boar semen. We also treated curcumin with/without phthalates in the boar semen. Fresh boar semen was treated with phthalates and/or curcumin for examining sperm characteristics. Sperm characteristics, sperm motility, viability, mitochondrial activity, and membrane integrity were determined during storage of boar semen. Sperm motility and viability in dose-dependent manner decreased by di-n-butyl phthalate (DBP), mono-n-butyl phthalate (MBP) and di-2-ethylhexyl phthalate (DEHP, p<0.05). Phthalates also decreased mitochondrial activity and membrane integrity of sperm (p<0.05). However, sperm motility and viability were higher than untreated-curcumin when DBP, MBP and DEHP treated with a curcumin in boar semen (p<0.05). Mitochondrial activity and membrane integrity of sperm were higher in DBP- and MBP-treated semen with curcumin (p<0.05). In conclusion, phthalates can damage sperm viability and quality during the boar semen storage, and curcumin may protect the boar sperms from phthalates during storage term.
Keywords
Boar semen; phthalate; spermatozoa;
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1 Grande, S. W., Andrade, A. J., Talsness, C. E., Grote, K., Golombiewski, A., Sterner-Kock, A. and Chahoud, I. 2007. A dose-response study following in utero and lactational exposure to di-(2-ethylhexyl) phthalate (DEHP): reproductive effects on adult female offspring rats. Toxicology 229, 114-122.   DOI
2 Hannon, P. R. and Flaws, J. A. 2015. The effects of phthalates on the ovary. Front. Endocrinol. doi: 10.3389/fendo.2015.00008.   DOI
3 He, Y., Yue, Y., Zheng, X., Zhang, K., Chen, S. and Du, Z. 2015. Curcumin, inflammation, and chronic diseases: how are they linked? Molecules 20, 9183-9213.   DOI
4 Henley, D. V. and Korach, K. S. 2006. Endocrine-disrupting chemicals use distinct mechanisms of action to modulate endocrine system function. Endocrinology 147, 25-32.   DOI
5 Jekal, S. J., Park, M. S. and Kim, D. J. 2017. The combined effects of curcumin administration and 630 nm LED phototherapy against DNCB-induced atopic dermatitis-like skin lesions in BALB/c mice. Kor. J. Clin. Lab. Sci. 49, 150-160.   DOI
6 Kemal Duru, N., Morshedi, M. and Oehninger, S. 2000. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Fertil. Steril. 74, 1200-1207.   DOI
7 Ko, E. Y., Sabanegh, E. S. Jr. and Agarwal, A. 2014. Male infertility testing: reactive oxygen species and antioxidant capacity. Fertil. Steril. 102, 1518-27.   DOI
8 Kumar, P., Kumar, D., Sikka, P. and Singh, P. 2015. Sericin supplementation improves semen freezability of buffalo bulls by minimizing oxidative stress during cryopreservation. Anim. Reprod. Sci. 152, 26-31.   DOI
9 Lee, Y. S., Lee, S. and Yang, B. K. 2018. Effects of bisphenol S on viability and reactive oxygen species of the sperm and ovarian granulosa cells in pigs. Ann. Anim. Resour. Sci. 29, 166-171.   DOI
10 Lonare, M., Kumar, M., Raut, S., More, A., Doltade, S., Badgujar, P. and Telang, A. 2015. Evaluation of ameliorative effect of curcumin on imidacloprid-induced male reproductive toxicity in wistar rats. Environ. Toxicol. doi: 10.1002/tox.22132.   DOI
11 Maffini, M. V., Rubin, B. S., Sonnenschein, C. and Soto, A. M. 2006. Endocrine disruptors and reproductive health: the case of bisphenol-A. Mol. Cell Endocrinol. 254-255, 179-186.   DOI
12 Martinez-Arguelles, D. B. and Papadopoulos, V. 2015. Mechanisms mediating environmental chemical-induced endocrine disruption in the adrenal gland. Front. Endocrinol. doi: 10.3389/fendo.2015.00029.   DOI
13 Muthumani, M. and Miltonprabu, S. 2015. Ameliorative efficacy of tetrahydrocurcumin against arsenic induced oxidative damage, dyslipidemia and hepatic mitochondrial toxicity in rats. Chem. Biol. Interact. 235, 95-105.   DOI
14 Naz, R. K. and Lough, M. 2014. Curcumin as a potential non-steroidal contraceptive with spermicidal and microbicidal properties. Eur. J. Obstet. Gynecol. Reprod. Biol. 176, 142-148   DOI
15 Oguzturk, H., Ciftci, O., Aydin, M., Timurkaan, N., Beytur, A. and Yilmaz, F. 2012. Ameliorative effects of curcumin against acute cadmium toxicity on male reproductive system in rats. Andrologia 44, 243-249.   DOI
16 Sariozkan, S., Bucak, M. N., Tuncer, P. B., Ulutas, P. A. and Bilgen, A. 2009. The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation. Cryobiology 58, 134-138.   DOI
17 Patel, S., Zhou, C., Rattan, S. and Flaws, J. A. 2015. The Effects of Endocrine Disrupting Chemicals on the Ovary. Biol. Reprod. doi: 10.1095/biolreprod.115.130336.   DOI
18 Pereira, C., Mapuskar, K. and Rao, C. V. 2006. Chronic toxicity of diethyl phthalate in male Wistar rats--a dose-response study. Regul. Toxicol. Pharmacol. 45, 169-177.   DOI
19 Aggarwal, B. B., Sundaram, C., Malani, N, and Ichikawa, H. 2007. Curcumin: The Indian solid gold. Adv. Exp. Med. Biol. 595, 1-75.   DOI
20 Sanocka, D. and Kurpisz, M. 2004. Reactive oxygen species and sperm cells. Reprod. Biol. Endocrinol. 2, 12.   DOI
21 Schecter, A., Lorber, M., Guo, Y., Wu, Q., Yun, S. H., Kannan, K., Hommel, M., Imran, N., Hynan, L. S., Cheng, D., Colacino, J. A. and Birnbaum, L. S. 2013. Phthalate concentrations and dietary exposure from food purchased in New York State. Environ. Health Perspect. 121, 473-494.   DOI
22 Schug, T. T., Blawas, A. M., Gray, K., Heindel, J. J. and Lawler, C. P. 2015. Elucidating the Links between Endocrine Disruptors and Neurodevelopment. Endocrinology 156, 1941-1951.   DOI
23 Serrano, S. E., Braun, J., Trasande, L., Dills, R. and Sathyanarayana, S. 2014. Phthalates and diet: a review of the food monitoring and epidemiology data. Environ. Health. doi: 10.1186/1476-069X-13-43.   DOI
24 Rithaporn, T., Monga, M. and Rajasekaran, M. 2003. Curcumin: a potential vaginal contraceptive. Contraception 68, 219-223.   DOI
25 Shafiei, M., Forouzanfar, M., Hosseini, S. M. and Esfahani, M. H. 2015. The effect of superoxide dismutase mimetic and catalase on the quality of postthawed goat semen. Theriogenology 83, 1321-1327.   DOI
26 Sharpe, R. M. 2006. Pathways of endocrine disruption during male sexual differentiation and masculinization. Best Pract. Res. Clin. Endocrinol. Metab. 20, 91-110.   DOI
27 Smith, C. A., Macdonald, A. and Holahan, M. R. 2011. Acute postnatal exposure to di (2- ethylhexyl) phthalate adversely impacts hippocampal development in the male rat. Neuroscience 193, 100-108.   DOI
28 Ciftci, O., Ozdemir, I., Tanyildizi, S., Yildiz, S. and Oguzturk, H. 2011. Antioxidative effects of curcumin, ${\beta}$-myrcene and 1,8-cineole against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in rat liver. Toxicol. Ind. Health 27, 447-453.   DOI
29 Chanapiwat, T. and Kaeoket, K. 2015. The effect of curcuma longa extracted (curcumin) on the quality of cryopreserved boar semen. Anim. Sci. J. 86, 863-868.   DOI
30 Choi, J. S. 2019. Analysis of toxicity in endometrial cells exposed phthalate. Kor. J. Clin. Lab. Sci. 51, 86-92.   DOI
31 Colborn, T. 2004. Commentary: setting aside tradition when dealing with endocrine disruptors. ILAR J. 45, 394-400.   DOI
32 Du, L., Li, G., Liu, M., Li, Y., Yin, S., Zhao, J. and Zhang, X. 2015. Evaluation of DNA damage and antioxidant system induced by di-n-butyl phthalates exposure in earthworms (Eisenia fetida). Ecotoxicol. Environ. Saf. 115, 75-82.   DOI
33 El-Bahr, S. M. 2013. Curcumin regulates gene expression of insulin like growth factor, B-cell CLL/lymphoma 2 and antioxidant enzymes in streptozotocin induced diabetic rats. BMC Complem. Altern. M. 13, 368-379.   DOI
34 Funahashi, H. and Sano, T. 2005. Select antioxidants improve the function of extended boar semen stored at 10 degrees C. Theriogenology 63, 1605-1616.   DOI
35 Albert, O. and Jegou, B. 2013. A critical assessment of the endocrine susceptibility of the human testis to phthalates from fetal life to adulthood. Hum. Reprod. Update 20, 231-249.   DOI
36 Ghosh, S., Banerjee, S. and Sil, P. C. 2015. The beneficial role of curcumin on inflammation, diabetes and neurodegenerative disease: A recent update. Food Chem. Toxicol. 83, 111-124.   DOI
37 Wittassek, M., Koch, H. M., Angerer, J. and Bruning, T. 2011. Assessing exposure to phthalates - The human biomonitoring approach. Mol. Nutr. Food Res. 55, 7-31.   DOI
38 Tayyem, R. F., Heath, D. D., Al-Delaimy, W. K. and Rock, C. L. 2006. Curcumin content of turmeric and curry powders. Nutr. Cancer 55, 126-31.   DOI
39 Tvrda, E., Kovacik, A., Tusimova, E., Massanyi, P. and Lukac, N. 2015. Resveratrol offers protection to oxidative stress induced by ferrous ascorbate in bovine spermatozoa. J. Environ. Sci. Health A Tox. Hazard Subst. Environ. Eng. 50, 1440-1451.   DOI
40 Vallianou, N. G., Evangelopoulos, A., Schizas, N. and Kazazis, C. 2015. Potential anticancer properties and mechanisms of action of curcumin. Anticancer Res. 35, 645-651.
41 Zhang, J. F., Hu, Z. P., Lu, C. H., Yang, M. X., Zhang, L. L. and Wang, T. 2015. Dietary curcumin supplementation protects against heat-stress-impaired growth performance of broilers possibly through a mitochondrial pathway. J. Anim. Sci. 93, 1656-1665.   DOI
42 Gobbo, M. G., Costa, C. F., Silva, D. G., de Almeida, E. A. and Goes, R. M. 2015. Effect of melatonin intake on oxidative stress biomarkers in male reproductive organs of rats under experimental diabetes. Oxid. Med. Cell. Longev. doi: 10.1155/2015/614579.   DOI