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http://dx.doi.org/10.5352/JLS.2019.29.2.173

Protective Effect of the Ethyl Acetate-fraction of Methanol Extract of Ophiophogon japonicus on Amyloid beta Peptide-induced Cytotoxicity in PC12 Cells  

Moon, Ja-Young (Department of Bio-Health Science, College of Natural Sciences, Changwon National University)
Kim, Eun-Sook (Department of Bio-Health Science, College of Natural Sciences, Changwon National University)
Choi, Soo-Jin (Department of Bio-Health Science, College of Natural Sciences, Changwon National University)
Kim, Jin-Ik (Department of Bio-Health Science, College of Natural Sciences, Changwon National University)
Choi, Nack-Shik (KPROTEC INC.)
Lee, Kyoung (Department of Bio-Health Science, College of Natural Sciences, Changwon National University)
Park, Woo-Jin (School of Life Sciences, Kwangju Institute of Science and Technology)
Choi, Young-Whan (Department of Horticultural Bioscience, College of Natural Resource and Life Sciences, Pusan National University)
Publication Information
Journal of Life Science / v.29, no.2, 2019 , pp. 173-180 More about this Journal
Abstract
Amyloid ${\beta}$-protein ($A{\beta}$) is the principal component of senile plaques characteristic of Alzheimer's disease (AD) and elicits a toxic effect on neurons in vitro and in vivo. Many environmental factors, including antioxidants and proteoglycans, modify $A{\beta}$ toxicity. It is worthwhile to isolate novel natural compounds that could prove therapeutic for patients with AD without causing detrimental side effects. In this study, we investigated the in vitro neuroprotective effects of the ethyl acetate fraction of methanol extract of Ophiophogon japonicas (OJEA fraction). We used an MTT reduction assay to detect protective effects of the OJEA fraction on $A{\beta}_{25-35}$-induced cytotoxicity to PC12 cells. We also used a cell-based ${\beta}$-secretase assay system to investigate the inhibitory effect of the OJEA fraction on ${\beta}$-secretase activity. In addition, we performed an in vitro lipid peroxidation assay to evaluate the protective effect of the OJEA fraction against oxidative stress induced by $A{\beta}_{25-35}$ in PC12 cells. The OJEA fraction had strong protective effects against $A{\beta}_{25-35}$-induced cytotoxicity to PC12 cells and was strongly inhibitory to ${\beta}$-secretase activity, which resulted in the attenuation of $A{\beta}$ generation. In addition, the OJEA fraction significantly decreased malondialdehyde (MDA) content, which is induced by the exposure of PC12 cells to $A{\beta}_{25-35}$. Our results suggested that the OJEA fraction contained active compounds exhibiting a neuroprotective effect on $A{\beta}$ toxicity.
Keywords
Amyloid beta ($A{\beta}$) protein; b-site APP cleaving enzyme (BACE); malondialdehyde; Ophiophogon japonicus; PC12 cell; ${\beta}$-secretase;
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