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http://dx.doi.org/10.5352/JLS.2019.29.2.158

Isolation of an Agarase-producing Persicobacter sp. DH-3 and Characterization of its β-agarase  

Heo, Da-Hye (Department of Green-Chemistry Convergence Engineering, Graduate School, Silla University)
Lee, Dong-Geun (Major in Pharmaceutical Engineering, Division of Bioindustry, Silla University)
Lee, Sang-Hyeon (Department of Green-Chemistry Convergence Engineering, Graduate School, Silla University)
Publication Information
Journal of Life Science / v.29, no.2, 2019 , pp. 158-163 More about this Journal
Abstract
The purpose of this study was to isolate a new marine agarase-producing bacterium. Agarase can hydrolyze agar and agarose to produce agarooligosaccharides or neoagarooligosaccharides, which possess many physiological functions. Strain DH-3 was isolated from seawater collected from the coast of Yeosu at Jeollanam province, Korea. A 16S rDNA sequence analysis showed this strain to be Persicobacter sp. DH-3. Extracellular agarase was prepared from culture media of Persicobacter sp. DH-3 and used for characterization. Relative activities at 20, 30, 40, 50, 60, and $70^{\circ}C$ were 50, 55, 70, 100, 90, and 50%, respectively. Relative activities at pH 5, 6, 7, and 8 were 75, 100, 90, and 75%, respectively. The enzyme showed maximum activity at $50^{\circ}C$ in a 20 mM Tris-HCl buffer at pH 6. This enzyme could be useful, as agar is in liquid state at $50^{\circ}C$. Agarase activities were maintained at 80% or more for 2 hr at 20, 30, and $40^{\circ}C$. Thin layer chromatography analysis suggested that Persicobacter sp. DH-3 produced extracellular ${\beta}$-agarases as it hydrolyzed agarose to produce neoagarohexaose and neoagarotetraose. In addition, zymogram analysis confirmed that Persicobacter sp. DH-3 produces at least three agar-degrading enzymes with molecular weights of 45, 70, and 140 kDa. Therefore, it is expected that agarases from Persicobacter sp. DH-3 could be used to produce functional neoagarooligosaccharides.
Keywords
Agar-degrading bacterium; ${\beta}$-agarase; neoagarooligosaccharides; Persicobacter sp. DH-3; zymogram;
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