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http://dx.doi.org/10.5352/JLS.2019.29.1.90

Antibacterial and Antibiofilm Activities of Diospyros malabarica Stem Extract against Streptococcus mutans  

Kim, Hye Soo (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Lee, Sang Woo (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology)
Sydara, Kongmany (Institute of Traditional Medicine, Ministry of Health, Vientiane Capital)
Cho, Soo Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Life Science / v.29, no.1, 2019 , pp. 90-96 More about this Journal
Abstract
The objective of this study was to evaluate the potential of Diospyros malabarica stem extract, a natural materials, in oral health material. With this aim in mind, thin layer chromatography (TLC), TLC-bioautography, high-performance liquid chromatography (HPLC), electrospray ionization-mass spectrometry (ESI-MS), scanning electron microscopy (SEM), and real-time qPCR were performed. The antibacterial activity of D. malabarica stem extract against Streptococcus mutans KCTC3065 was confirmed in an n-hexane fraction with low polarity. The molecular weight of the antibacterial compound was estimated to be 188 by ESI-MS analysis. The inhibitory effects of the extract on biofilm formation and gene expression related to biofilm formation of S. mutans were determined by SEM and real-time PCR analysis. The extract inhibited the formation of S. mutans biofilms at D. malabarica stem extract concentrations of 1 mg/ml, as shown by SEM. The real-time PCR analysis showed that the expression of the gtfC gene, which is associated with biofilm formation, was significantly decreased in a dose-dependent manner. Based on the above results, it can be concluded that D. malabarica stem extracts, a natural materials, can be used in oral health products to suppress the formation of biofilms by inhibiting tooth adhesion of S. mutans, a causative agent of dental caries.
Keywords
Biofilm formation; Diospyros malabarica stems; gtfB; gtfC; S. mutans;
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