Browse > Article
http://dx.doi.org/10.5352/JLS.2019.29.1.29

The Antimicrobial Activity of Fermented Extracts from Korean Dendropanax morbifera  

Lee, Jae-Yeul (Department of Applied Chemistry, Kumoh National Institute of Technology)
Park, Tae-Hee (Department of Applied Chemistry, Kumoh National Institute of Technology)
Park, Se-Ho (Department of Applied Chemistry, Kumoh National Institute of Technology)
Yang, Seun-Ah (Department of Food Science and Technology, Keimyung University)
Jhee, Kwang-Hwan (Department of Applied Chemistry, Kumoh National Institute of Technology)
Publication Information
Journal of Life Science / v.29, no.1, 2019 , pp. 29-36 More about this Journal
Abstract
We investigated the fermentation conditions for extracts of leave/branches and sap from Korean Dendropanax morbifera (D. morbifera) using Lactobacillus plantarum (L. plantarum) ilchiwhangchil 1785 and L. plantarum ilchiwhangchil 2020. Log growth phase cultured L. plantarum ilchiwhangchil 1785 and L. plantarum ilchiwhangchil 2020 were used for fermentation. The pH and growth of the microorganisms in broth were monitored during the fermentation period. The results revealed that the optimum fermentation conditions for 20 wt% of leave/branches extracts and 1 wt% of sap extract was 2 days incubation at $37^{\circ}C$. The minimum inhibitory concentration (MIC) method and a disk diffusion assay were used to evaluate the antimicrobial activity of the fermented extracts of the leave/branches and sap against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The antimicrobial activity increased in all three strains grown on the medium containing fermented extracts of the leave/branches and sap as compared with that of the strains grown on medium containing non fermented extracts. Furthermore, the antimicrobial activity increased in proportion to the contents of the fermented extracts. Our data suggest that fermented extracts of leave/branches and sap of D. morbifera have applications as natural bio functional materials, such as preservatives, cosmetic materials, and natural packaging materials.
Keywords
Antimicrobial activity; Dendropanax morbifera; fermentation; Lactobacillus plantarum ilchiwhangchil 1785; Lactobacillus plantarum ilchiwhangchil 2020;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Bernart, M. W., Cardellina, J. H., Balaschak, M. S., Alexander, M. R., Shoemaker, R. H. and Boyd, M. R. 1996. Cytotoxic falcarinol oxylipins from Dendropanax arboreus. J. Nat. Prod. 59, 748-753.   DOI
2 Chung, I. M., Seo, S. H., Kang, E. Y., Park, S. D., Park, W. H. and Moon, H. I. 2009. Chemical composition and larvicidal effects of essential oil of Dendropanax morbifera against Aedes aegypti L. Biochem. Syst. Ecol. 37, 470-473.   DOI
3 Cross, M. L., Stevenson, L. M. and Gill, H. S. 2001. Anti-allergy properties of fermented foods: an important immunoregularoty mechanism of lactic acid bacteria? Int. Immunopharmacol. 1, 891-901.   DOI
4 Fu, W. and Mathews, A. P. 1999. Lactic acid production from lactose by Lactobacillus plantarum: kinetic model and effects of pH, substrate, and oxygen. Biochem. Eng. J. 3, 163-170.   DOI
5 Hong, W. S. and Yoon, S. 1989. The effects of low temperature heating and mustard oil on the Kimchi fermentation. Kor. J. Food Sci. Technol. 21, 331-337.
6 Huhtanen, C. N. 1980. Inhibition of Clostridium botulinum by spice extracts and aliphatic alcohols. J. Food Sci. 43, 195-196.
7 Hyun, T. K., Kim, M. O., Lee, H., Kim, Y., Kim, E. and Kim, J. S. 2013. Evaluation of anti-oxidant and anti-cancer properties of Dendropanax morbifera Leveille. Food Chem. 141, 1947-1955.   DOI
8 Im, K. J., Jang, S. B. and Yoo, D. Y. 2015. Anti-cancer effects of Dendropanax morbifera extract in MCF-7 and MDA-MB-231 cells. J. Kor. Obstet. Gynecol. 28, 26-39.   DOI
9 Jeong, B. S., Jo, J. S., Pyo, B. S. and Hwang, B. 1995. Studies on the distribution of Dendropanax morbifera and component analysis of the golden lacquer. Kor. J. Biotechnol. Bioeng. 10, 393-400.
10 Jung, H. J., Park, S. H., Seo, H. A., Kim, Y. J., Cho, J. I., Park, S. S., Song, D. S. and Kim, K. S. 2005. Differentiation of four major gram-negative foodborne pathogenic bacterial genera by using ERIC-PCR genomic fingerprinting. Kor. J. Food Sci. Technol. 37, 1005-1011.
11 Kang, D. H. and Kim, H. S. 2011. Functionality analysis of Korean medicine fermented by Lactobacillus strains. Kor. J. Microbiol. Biotechnol. 39, 259-265.
12 Karovicova, J. and Kohajdova, Z. 2003. Lactic acid fermented vegetable juices. Hortic. Sci. 30, 152-158.   DOI
13 Koho, K. H. 1996. Antimicrobial effects of short-chain fatty acids against Saccaromyces cerevisiae. Foods Biotechnol. 5, 42-47.
14 Kolodziejczyk, K., Sojka, M., Abadias, M., Vinas, I., Guyot, S. and Baron, A. 2013. Polyphenol composition, antioxidant capacity, and antimicrobial activity of the extracts obtained from industrial sour cherry pomace. Ind. Crop. Prod. 51, 279-288.   DOI
15 Lee, B. W. and Shin, D. H. 1991. Screening of natural antimicrobial plant extract on food spoilage microorganisms. Kor. J. Food Sci. Technol. 23, 200-204.
16 Lee, S. H., Lee, H. S., Park, Y. S., Hwang, B., Kim, J. H. and Lee, H. Y. 2002. Screening of immune activation activities in the leaves of Dendropanax morbifera Lev. Kor. J. Med. Crop Sci. 10, 109-115.
17 Li, X. Z., Livermore, D. M. and Nikaido, H. 1994. Role of efflux pump(s) in intrinsic resistance of Pseudomonas aeruginosa: resistance to tetracycline, chloramphenicol, and norfloxacin. Antimicrob. Agents Chemother. 38, 1732-1741.   DOI
18 Mo, J. H. and Oh, S. J. 2013. Tyrosinase inhibitory activity and melanin production inhibitory activity of the methanol extract and fractions from Dendropanax morbifera Lev. Kor. J. Aesthet. Cosmetol. 11, 275-280.
19 Perdigon, G., Fuller, R. and Raya, R. 2001. Lactic acid bacteria and their effect on the immune system. Curr. Issues Intest. Microbiol. 2, 27-42.
20 Olsen, A., Halm, M. and Jakobsen, M. 1995. The antimicrobial activity of lactic acid bacteria from fermented maize (kenkey) and their interactions during fermentation. J. Appl. Bacteriol. 79, 506-512.   DOI
21 Poole, K. 2001. Multidrug efflux pumps and antimicrobial resistance in Pseudomonas aeruginosa and related organisms, J. Mol. Microbiol. Biotechnol. 3, 255-264.
22 Rafter, J. 2002. Lactic acid bacteria and cancer: mechanistic perspective. Brit. J. Nutr. 88, S89-S94.   DOI
23 Rozes, N. and Peres, C. 1998. Effects of phenolic compounds on the growth and the fatty acid composition of Lactobacillus plantarum. Appl. Microbiol. Biotechnol. 49, 108-111.   DOI
24 Shin, D. C., Kim, G. C., Song, S. Y., Kim, H. J., Yang, J. C. and Kim, B. A. 2013. Antioxidant and antiaging activities of complex supercritical fluid extracts from Dendropanax morbifera, Corni fructus and Lycii fructus. Kor. J. Herbol. 28, 95-100.
25 Song, J. H., Kang, H. B., Kim, J. H., Kwak, S. M., Sung, G. J., Park, S. H., Jeong, J. H., Kim, H. H., Lee, J. M., Jun, W. J., Kim, Y. J. and Choi, K. C. 2018. Antiobesity and cholesterol-lowering effects of Dendropanax morbifera water extracts in mouse 3T3-L1 cells. J. Med. Food 21, 793-800.   DOI