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http://dx.doi.org/10.5352/JLS.2019.29.11.1227

Inhibitory Effects of Prunus mume Solvent Fractions on Human Colon Cancer Cells  

Kim, Jeong-Ho (Department of Food Science and Technology, Kyungpook National University)
Cho, Hyun-Dong (Industry-Academy Cooperation, Dong-A University)
Won, Yeong-Seon (Department of Biotechnology, Dong-A University)
Heo, Ji-An (Department of Biotechnology, Dong-A University)
Kim, Ji-Young (Department of Biotechnology, Dong-A University)
Kim, Hwi-Gon (Department of Biotechnology, Dong-A University)
Han, Sim-Hee (Department of Forest Genetic Bio-resources, National Institute of Forest Science)
Moon, Kwang-Deog (Department of Food Science and Technology, Kyungpook National University)
Seo, Kwon-Il (Department of Biotechnology, Dong-A University)
Publication Information
Journal of Life Science / v.29, no.11, 2019 , pp. 1227-1234 More about this Journal
Abstract
Prunus mume, also known as maesil, is a popular fruit consumed in East Asia (Korea, Japan, and China). It contains high amounts of organic acids, minerals, and polyphenols and has been used as a medication for fever, vomiting, and detoxification. In this study, the anti-proliferative and apoptotic effects of solvent fractions from maesil were evaluated using sulforhodamine B (SRB) assays, morphological evaluations, Hoechst 33258 staining, and western blotting. Addition of the maesil methanol fraction (MMF) and the maesil butanol fraction (MBF) significantly and dose-dependently decreased the cell viability of HT-29 human colon cancer cells. Colony-forming assays confirmed that the MMF and MBF treatments decreased colony numbers when compared with untreated control cells. Treatment of HT-29 cells with MMF and MBF caused a distortion of the cell morphology to a shrunken cell mass. Treatment with MMF and MBF also dose-dependently increased nuclear condensation and the formation of apoptotic bodies in HT-29 cells. Treatment with MMF and MBF significantly and dosedependently increased the expression of Bax (a pro-apoptotic protein), caspase-3, and poly ADP-ribose polymerase (PARP) and decreased the expression of Bcl-2 (an anti-apoptotic protein). MMF significantly and dose-dependently inhibited cell proliferation induced by bisphenol A, an environmental hormone. Therefore, MMF may have potential use as a functional food and as a possible therapeutic agent for the prevention of colon cancer.
Keywords
Apoptosis; colon cancer; environmental hormone; Prunus mume; solvent fraction;
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