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http://dx.doi.org/10.5352/JLS.2019.29.10.1159

Establishment of an In Vitro TCD (Testosterone Compound Detection) System  

Lee, Dong-Geun (Major in Pharmaceutical Engineering, Division of Bioindustry, Silla University)
Jo, Jung-Kwon (Department of Bioscience, Graduate School, Silla University)
Lee, Sang-Hyeon (Major in Pharmaceutical Engineering, Division of Bioindustry, Silla University)
Publication Information
Journal of Life Science / v.29, no.10, 2019 , pp. 1159-1163 More about this Journal
Abstract
Although there is a growing interest in male menopause, a phenomenon associated with male hormone depletion, current kits using antibodies to quantify male hormones are expensive. In this study, we constructed an in vitro system for verifying the activity or concentration of male steroid hormones using a transcriptional activity test. A reporter plasmid, pGL2-Neo-ARE-AdE1BTATA, which reacts to testosterone, was constructed. In this plasmid, the ARE-AdE1bTATA sequences can be bounded by the testosterone - androgen receptor complex to express luciferase as a reporter. Then, a stable transfection was performed on the human prostate cancer cell line, LNcap-LN3. The constructed LNcap-LN3/pGL2-Neo-ARE-AdE1BTATA testosterone compound detection (TCD) system showed quantitatively proportional luciferase activities to concentrations of $10^{-13}$ to $10^{-8}M$ of standard testosterone. The established in vitro TCD system will contribute to the development of materials for health/functional foods and drugs as it will be possible to search en masse for testosterone-like or testosterone-inhibiting substances derived from natural materials.
Keywords
Androgen; luciferase; reporter; testosterone;
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