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http://dx.doi.org/10.5352/JLS.2019.29.10.1104

Inhibitory Effects of Locusta migratoria Ethanol Extracts on RANKL-induced Osteoclast Differentiation  

Baek, Minhee (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Seo, Minchul (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Joon Ha (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Hwa Jeong (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, In-Woo (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Sun Young (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Mi-Ae (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Sunghyun (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Hwang, Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Publication Information
Journal of Life Science / v.29, no.10, 2019 , pp. 1104-1110 More about this Journal
Abstract
Recently, there has been an increase in the elderly population of the world. Consequently, bone metabolic diseases such as osteoporosis are emerging as a social problem. Osteoclasts play a role in bone resorption, and osteoporosis is induced when bone resorption occurs excessively. Because currently used bone resorption inhibitors may cause side effects when used for a long period of time, it is necessary to develop a new material that effectively inhibits osteoclast differentiation. This study aimed to confirm the inhibitory effect of ethanol extract of Locusta migratoria on RANKL-induced osteoclast differentiation and its mechanism. The toxicity and proliferation effects of LME on RAW264.7 osteoclasts were measured by an MTS assay. There was no cytotoxicity or proliferation when the osteoclasts were treated with up to $2,000{\mu}g/ml$ of LME. In order to confirm the effect of LME on the differentiation of osteoclasts, osteoclasts were treated with RANKL alone or with LME for 3 days. As a result of a TRAP (tartrate-resistant acid phosphatase) assay, the increasing osteoclast differentiation by RANKL decreased in a concentration-dependent manner with the treatment of LME. In addition, LME suppressed the expression of differentiation-related marker genes (TRAP, RANK, NFATc1, and CK) and proteins (NFATc1 and c-Src) that had been increased by RANKL. Also, LME influenced the $NF-{\kappa}B$, ERK and JNK signaling pathways, resulting in the inhibition of osteoclast differentiation. These results suggest that LME may be used as a novel functional material for the prevention and treatment of osteoporosis by playing a role in inhibiting bone absorption.
Keywords
Bone resorption; Locusta migratoria; osteoclastogenesis; osteoporosis; RANKL;
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