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http://dx.doi.org/10.5352/JLS.2019.29.10.1062

Activity-guided Purification of N-benzyl-N-methyldecan-1-amine from Garlic and Its Antitumor Activity against CT-26 Colorectal Carcinoma in BALB/C Mice  

Seetharaman, Rajasekar (Department of Horticultural Bioscience, Pusan National University)
Choi, Seong Mi (Department of Horticultural Bioscience, Pusan National University)
Guo, Lu (Department of Horticultural Bioscience, Pusan National University)
Cui, Zheng Wei (Department of Horticultural Bioscience, Pusan National University)
Otgonbayar, Duuriimaa (Department of Horticultural Bioscience, Pusan National University)
Park, Ju Ha (Department of Horticultural Bioscience, Pusan National University)
Kwon, Young-Seok (Onion and Garlic Lab, Vegetable Research Division, National Institute of Horticultural & Herbal Science)
Kwak, Jung Ho (Onion and Garlic Lab, Vegetable Research Division, National Institute of Horticultural & Herbal Science)
Kwon, Young Hee (Chungcheongbuk-do Agricultural Research & Extension Services)
Min, Ji Hyun (Chungcheongbuk-do Agricultural Research & Extension Services)
Kang, Jum Soon (Department of Horticultural Bioscience, Pusan National University)
Choi, Young Whan (Department of Horticultural Bioscience, Pusan National University)
Publication Information
Journal of Life Science / v.29, no.10, 2019 , pp. 1062-1070 More about this Journal
Abstract
A components of garlic (Allium sativum) have anti-proliferative effects against various types of cancer. We aimed to investigate the capacity of garlic compounds to anti-tumor on a various cancer cell lines. Fractionation of garlic extract, guided by antiproliferative activity against human gastric cancer (AGS) cells, has resulted in the isolation of N-benzyl-N-methyldecan-1-amine (NBNMA). We investigated the effect of newly isolated NBNMA from garlic cloves on the inhibition of the growth of CT-26, AGS, HepG2, HCT-116, MCF7, B16F10, and Sarcoma-180 cells for in vitro and CT-26 colon carcinoma cells in vivo. NBNMA exhibited an antiproliferative effect in CT-26 cells by apoptotic cell death. NBNMA exhibited down-regulation of anti-apoptotic Bcl-2 proteins and up-regulation of apoptotic Bad protein expression in western blot analyses. In addition, NBNMA meagre activated caspase 3 and caspase 9, initiator caspases of the extrinsic and intrinsic pathways of apoptosis. NBNMA treatment at a dose of 10 mg/kg for 21 days in experimental mice implanted with tumors resulted in significant reduction of the tumor weight (43%). NBNMA exhibited both in vitro and in vivo anticancer activity. These results indicate that NBNMA has promising potential to become a novel anticancer agent from garlic cloves for the treatment of colon carcinoma cancer.
Keywords
Activity-guided purification; anti-cancer; colorectal cancer; garlic; N-benzyl-N-methyldecan-1-amine;
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