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http://dx.doi.org/10.5352/JLS.2018.28.9.1118

Characteristics and Diagnostic Methods of Streptococcosis Causing Disease in Aquaculture  

Kim, Dong-Hwi (Marine Pathogenic microbes Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, Jeju National University)
Heo, Moon-Soo (Marine Pathogenic microbes Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, Jeju National University)
Publication Information
Journal of Life Science / v.28, no.9, 2018 , pp. 1118-1126 More about this Journal
Abstract
In this study, investigated the general characteristics and diagnostic methods types of streptococcosis among various fish disease pathogens that caused a lot of economic damaged to aquaculture fish based on the previous research paper. Streptococcosis infection of fish is considered a reemerging disease affecting a variety of wild and cultured fish throughout the world. Calssifiacation of Gram positive cocci based on DNA-DNA hybridization coupled with 16S sequencing has shown that at least five different species are considered of significance as fish pathogens: Lactococcus garvieae, L. piscium, Streptococcus iniae, S. agalactiae, S. paruberis, Vagococcus salmoninarum. Symptoms of infection with streptococcosis disease such as body color change, eyeball abnormality, gill discoloration, bleeding, abdominal distension, swelling of the kidney and spleen. In addition, it usually occurs from June to October when the water temperature rise a lot of fish death. Currently, 16S rRNA, 16S-23S rRNA intergenic spacer region (ISR), Random Amplified polymorphic DNA (RAPD), Ribotyion (RT), Loop-mediated isothermal amplification (LAMP) are among the methods for diagnosing streptococcosis. Among them, the LAMP method, which is high applicable to the aquaculture farm has attracted the spotlight, but due to problems such as confirmation of results. This seems to minimize the economic loss of streptococcosis which complements the problem so that it can be easily used from the diagnosis to the results confirmation.
Keywords
Aquaculture; diagnostic; Loop-mediated isothermal amplification (LAMP); pathogens; Streptococcosis;
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