Browse > Article
http://dx.doi.org/10.5352/JLS.2018.28.8.923

Physicochemical Properties and Biological Activities of Tenebrio molitor Fermented by Several Kinds of Micro-organisms  

Jang, Sung-Ho (Department of Biotechnology, Dong-A University)
Sim, So-Yeon (Department of Biotechnology, Dong-A University)
Ahn, Hee-Young (Department of Biotechnology, Dong-A University)
Seo, Kwon-Il (Department of Biotechnology, Dong-A University)
Cho, Young-Su (Department of Biotechnology, Dong-A University)
Publication Information
Journal of Life Science / v.28, no.8, 2018 , pp. 923-930 More about this Journal
Abstract
In this study, Tenebrio molitor (T. molitor) was fermented with Lactobacillus plantarum JBMI F3 (F3), Lactobacillus plantarum JBMI F5 (F5), Lactobacillus gasseri Ba9 (Ba9), Aspergillus kawachii KCCM 32819 (Ak), Saccharomyces cerevisiae KACC 93023 (Sc), and Bacillus subtilis KACC 91157 (Bs). After fermentation, the fermented products were extracted by water, ethanol, and methanol, and their physicochemical and biological properties were investigated. In a DPPH assay, the water extracts of the fermented products of T. molitor showed high antioxidant ability. Among the water extracts, the fermented product by Bs showed the highest DPPH radical scavenging activity. The total contents of phenolic compounds and flavonoids were highest in the fermented products by Ak and Bs, respectively. Reducing activity was detected the most high activity on ethanol extract of fermented product by Bs. The water extract of the fermented product by Bs exhibited strong enzymatic activity for fibrinogen and starch hydrolysis. Based on the observed physicochemical and biological properties, the fermented products of T. molitor by microorgansims can likely be applied as functional materials in various industries.
Keywords
Antioxidative activity; Bacillus subtilis; fermentation; fibrinolytic activity; Tenebrio molitor;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Jia, Z., Tang, M. and Wu, J. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64, 555-559   DOI
2 Lee, J. H., Um, Y. R., Shim, K. S., Jeon, W. K., Lee, J. H. and Ma, J. Y. 2009. Acute toxicity study on fermented Ssanghwa-tang extracts in mice. Kor. J. Orient. Int. Med. 30, 780-787.
3 Kaur, C. and Kapoor, H. C. 2002. Anti-oxidant activity and total phenolic content of some Asian vegetables. Int. J. Food Sci. Technol. 37, 153-161.   DOI
4 Kim, H. A., Lee, S. H., Choi, Y. C., Park, K. H., Hwang, J. S., Kim, N. J. and Nam, S. H. 2013. Comparison of fibrinolytic activity from Korean indigenous insects. J. Seric. Entomol. Sci. 51, 147-152.
5 Kim, S. Y., Son, Y. J., Kim, S. H., Kim, A. N., Lee, G. Y. and Hwang, I. K. 2015. Studies on oxidative stability of Tenebrio molitor Larvae during cold storage. Kor. J. Food Cook Sci. 31, 62-71   DOI
6 Chae, K. S., Jung, J. H., Yoon, H. H. and Son, R. H. 2014. Antioxidant activity and main volatile flavor components of mulberry wine fermented with Saccharomyces cerevisiae B-8. J. Kor. Soc. Food Sci. Nutr. 43, 1017-1024.   DOI
7 Lee, S. Y., Kim, J. H., Park, J. M., Lee, I. C. and Lee, J. Y. 2014. Antioxidant activity and inhibition activity against ${\alpha}$-amylase and ${\alpha}$-glucosidase of Smilax China L. Kor. J. Food Preserv. 21, 254-263.   DOI
8 Liu, S., Sun, J., Yu, L., Zhang, C., Bi, J., Zhu, F. and Yang, Q. 2012. Antioxidant activity and phenolic compounds of Holotrichia parallela Motschulsky extracts. Food Chem. 134, 1885-1891.   DOI
9 Jeong, M. E., Kang, S. A. and Cheong, C. 2014. Physicochemical characteristics of the mash quality of Cheongju prepared using different Nuruks. JKAIS. 15, 5150-5158.
10 Baek, M. H., Seo, M. C., Kim, M. A., Yun, E. Y. and Hwang, J. S. 2017. The antioxidant activities and hair-growth promotion effects of Tenebrio molitor Larvae extracts (TMEs). Saengmyeong Gwahag Hoeji 27, 1269-1275.
11 Kang, M. S., Kim, M. J., Han, J. S. and Kim, A. J. 2017. Fatty acid composition and anti-inflammatory effects of the freeze dried Tenebrio molitor Larva. Kor. J. Food Nutr. 30, 251-256.   DOI
12 Oomah, B. D., Cardador‐Martinez, A. and Loarca‐Pina, G. 2005. Phenolics and antioxidative activities in common beans (Phaseolus vulgaris L). J. Sci. Food Agric. 85, 935-942.   DOI
13 Phonesavanh, P., Kim, K. K., Lim, J. C., Lee, K. Y., Park, C. H. and Choe, M. 2015. Saccharification of Fagopyrum esculentum by amylase treatments increases phenolic compound content and antioxidant activity. J. East Asian Soc. Diet Life 25, 139-145.   DOI
14 Hwang, S. Y., Bae, G. K. and Choi, S. K. 2015. Preferences and purchase intention of Tenebrio molitor (Mealworm) according to cooking method. Culi. Sci. Hos. Res. 21, 100-115.
15 Swain, T. and Hillis, W. E. 1959. The phenolic constituents of Prunus domestica. I. The quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10, 63-68.   DOI
16 Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1204.
17 Yoo, J. M., Hwang, J. S., Goo, T. W. and Yun, E. Y. 2013. Comparative analysis of nutritional and harmful components in Korean and Chinese mealworms (Tenebrio molitor). J. Kor. Soc. Food Sci. Nutr. 42, 249-254.   DOI
18 An, S. I. 2005. Antioxidative and physiological activities of some phenolic compounds. Department of Food and Biotechnology Graduate School of Technology Hankyong National University.
19 Astrup, T. and Mullertz, S. 1991. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40, 346-351.
20 Bijoy, M., Jayati, S. and Prabir, K. Sarkar. 2008. Antioxidant activities of soybean as affected by Bacillus-fermentation to kinema. Food Res. Int. 41, 586-593   DOI
21 Chang, H. G. and Park, Y. S. 2003. Lactic acid fermentation and biological activities of Rubus coreanus. J. Appl. Biol. Chem. 46, 367-375.
22 Ahn, H. Y., Choe, D. J., Kim, B. K., Lee, J. H. and Cho, Y. S. 2015. Bioactive materials and antioxidant properties of fermented rice-bran extract. Saengmyeong Gwahag Hoeji 25, 1014-1020.
23 Chung, S. J., Lee, Y. H., Chung, J. H., Lee, B. R. and Han, D. M. 1995. Antifungal Effect and activity spectrum of crude antifungal proteins from hemolymph of larvae of Tenebrio molitor in Korea. Hanguk Kyun Hakoe Chi 23, 232-237.
24 Dibyangana, R., Tania, B., Suchita, M., Shrayan, K. D. and Suvroma, G. 2014. Production and partial purification of alpha amylase from Bacillus subtilis (MTCC 121) using solid state fermentation. Biochem. Res. Int. 2014, 568141.
25 Duncan, D. B. 1955. Multiple range and multiple F test. Biometrics 1, 1-42.
26 Huang, M. X., Ye, Y., Chen, Y. and Han, Y. L. 2012. Partial purification and characterization of fibrinolytic enzymes from yellow mealworm. Int. J. Pept. Res. Ther. 18, 153-161.   DOI
27 Cha, J. Y., Kim, Y. S., Ahn, H. Y., Kang, M. J., Heo, S. J. and Cho, Y. S. 2011. Biological activity and biochemical properties of silkworm (Bombyx mori L.) powder fermented with Bacillus subtilis and Aspergillus kawachii. Saengmyeong Gwahag Hoeji 21, 81-88.