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http://dx.doi.org/10.5352/JLS.2018.28.8.917

Analysis of Triterpene Glycoside Levels and Antioxidant Activity in the Different Shoot Tissues of Centella asiatica (L.) Urban  

Kil, Young Sook (Gyeongnam Oriental Anti-aging Institute)
Sin, Seung Mi (Gyeongnam Oriental Anti-aging Institute)
Lee, Dong Yeol (Gyeongnam Oriental Anti-aging Institute)
Jeong, Won Min (Gyeongnam Oriental Anti-aging Institute)
Yang, Ki jeung (Gyeongnam Oriental Anti-aging Institute)
Lee, Shin-Woo (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology)
Kim, Yun-Hee (Department of Biology Education, College of Education, IALS, Gyeongsang National University)
Goo, Young-Min (Gyeongnam Oriental Anti-aging Institute)
Publication Information
Journal of Life Science / v.28, no.8, 2018 , pp. 917-922 More about this Journal
Abstract
Centella asiatica is one of the local herbs that is claimed to possess various physiological effects. C. asiatica also accumulates large amounts of pentacyclic triterpenoid saponins known as centelloids. These terpenoids usually include asiatic acid, asiaticoside, madecassoside, and madecassic acids. In the present study, to understand the changes of triterpene glycoside levels in the different shoot tissues of C. asiatica during seasonal cultivation, we investigated the High-Performance Liquid Chromatography (HPLC) analysis via different extraction methods, such as water, 20% ethanol and methanol extracts. Significant increases were observed in the levels of madecassoside and asiaticoside in the leaf extracts by methanol compare with extracts using water or 20% ethanol. Additionally, we also analyzed the various antioxidant activity in the different shoot tissues of C. asiatica using different extracts, such as leaves, petioles and both materials. Among these petiole extracts showed high 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTs) scavenging activity in all extracts, whereas 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity exhibited high activity levels in the leaf part using methanol and ethanol extracts. Levels of total phenolics and flavonoid also showed the highest levels in the leaf tissues using all extracts such as methanol, ethanol and water. Our results indicated that the increased levels of triterpene glycoside and antioxidant activity in the leaf parts of the C. asiatica were indicating that useful metabolites were mainly maintained through seasonal cultivation such as madecassoside, asiaticoside.
Keywords
Antioxidant activity; Centella asiatica; different shoot tissues; seasonal cultivation; triterpene glycoside;
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