Browse > Article
http://dx.doi.org/10.5352/JLS.2018.28.4.415

Anti-proliferative and Pro-apoptotic Activities by Pomace of Schisandra chinensis (Turcz.) Baill. and Schizandrin  

Kim, Hyun-Ji (Department of Biological Sciences, Andong National University)
Seo, Yu-Mi (Department of Biological Sciences, Andong National University)
Lee, Eun-Ju (Department of Biological Sciences, Andong National University)
Chung, Chungwook (Department of Biological Sciences, Andong National University)
Sung, Hwa-Jung (Department of Food and Nutrition, Andong National University)
Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
Park, Jong-Yi (Gyeongbuk Institute for Bio Indudustry)
Kim, Jong-Sik (Department of Biological Sciences, Andong National University)
Publication Information
Journal of Life Science / v.28, no.4, 2018 , pp. 415-420 More about this Journal
Abstract
Schisandra chinensis (Turcz.) Baill. (omija) is often used in Chinese medicine to treat various human diseases, and is known to possess various bioactive components such as schizandrin and gomisin A. In the present study, we prepared ethanol extracts of pomace of Schisandra chinensis (PSC) and investigated their effects on cell viability and expression changes of pro-apoptotic genes such as ATF3, NAG-1 and p21 in human colorectal cancer HCT116 cells. PSC significantly reduced cell viability in a dose-dependent manner, and also dramatically induced the expression of ATF3, NAG-1 and p21 genes, with resveratrol used as a positive control. We also assessed the effects of pure compound schizandrin (SZ) derived from Schisandra chinensis on cell viability and expression of pro-apoptotic genes such as ATF3, NAG-1 and p21. The results showed that SZ also decreased cell viabilities in a dose-dependent manner and increased the expression of ATF3, NAG-1 and p21 genes. In addition, apoptosis was detected in SZ-treated HCT116 cells, which was confirmed with PARP cleavage. PARP cleavage was recovered in part by the transfection of NAG-1 siRNA. The results indicate that NAG-1 is one of the genes responsible for apoptosis induced by SZ. Overall, our findings may contribute to understanding the molecular mechanisms of anti-proliferative and pro-apoptotic activities mediated by PSC and SZ.
Keywords
Anti-proliferation; apoptosis; NAG-1; pomace of Schisandra chinensis; schizandrin;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Cho, Y. J., Ju, I. S., Kim, B. C., Lee, W. S., Kim, M. J., Lee, B. G., An, B. J., Kim, J. H. and Kwon, O. J. 2007. Biological activity of Omija (Schizandra chinensis Baillon) extracts. J. Kor. Soc. Appl. Biol. Chem. 50, 198-203.
2 Frank, G., Bottone, Jr. and Brenda, A. M. 2011. The dietary compounds resveratrol and genistein induce activating transcription factor 3 while suppressing inhibitor of DNA binding/differentiation-1. J. Med. Food 14, 584-593   DOI
3 Guon, T. G. and Chung, H. S. 2014. Effects of Nelumbo nucifera root extract on proliferation and apoptosis in HT-29 human colon cancer cells. J. East Asian Soc. Dietary Life. 24, 20-27.
4 Hu, X., Wang, J., Xia, Y, Simayi, M, Ikramullah, S., He, Y., Cui, S., Li, S. and Wushouer, Q. 2016. Resveratrol induces cell cycle arrest and apoptosis in human eosinophils from asthmatic individuals. Mol. Med. Rep. 14, 5231-5236.   DOI
5 Zhang, C., Gao, C., Kawauchi, J., Hashimoto, Y., Tsuchida N. and Kitajima, S. 2002. Transcriptional activation of the human stress-inducible transcriptional repressor ATF3 gene promoter by p53. Biochem. Biophys. Res. Commun. 297, 1302-1310.   DOI
6 Zhang, Z., Wu, L., Wang, J., Li, G., Feng, D., Zhang, B., Li, L., Yang, J., Ma, L. and Qin, H. 2014. Opposing effects of PI3K/Akt and Smad-dependent signaling pathways in NAG-1-induced glioblastoma cell apoptosis. PloS One 9, e96283.   DOI
7 Zhu, H., Zhang, L., Wang, G., He, Z., Zhao, Y., Xu, Y., Gao, Y. and Zhang, L. 2016. Sedative and hypnotic effects of supercritical carbon dioxide fluid extraction from Schisandra chinensis in mice. J. Food Drug Anal. 24, 831-838.   DOI
8 Kim, K. J., Lee, J., Park, Y. and Lee, S. H. 2015. ATF3 mediates anti-cancer activity of trans-10, cis-12-conjugated linoleic acid in human colon cancer cells. Biomol. Ther. (Seoul). 23, 134-140.   DOI
9 Kim, B. H., Cho, D. U., Han, K. S. and Bae, Y. L. 2011. Efficiency analysis of Schisandra tea using image & acoustic signal processing. Journal of Korea Academia Industrial Cooperation Society 12, 2975-2981.   DOI
10 Kim, J. S. and Choi, S. 2008. Physicochemical properties and antioxidative activities of Omija (Schizandra chinensis Bailon). Kor. J. Food Nutr. 21, 35-42.
11 Kim, M. S., Sung, H. J., Park, J. Y. and Sohn, H. Y. 2017. Evaluation of anti-oxidant, anti-microbial and anti-thrombosis activities of fruit, seed and pomace of Schizandra chinensis Baillon. J. Life Sci. 27, 131-138.   DOI
12 Lim, J. H., Park, J. W., Min, D. S., Chang, J. S., Lee, Y. H., Park, Y. B., Choi, K. S. and Kwon, T. K. 2007. NAG-1 up-regulation mediated by EGR-1 and p53 is critical for quercetin-induced apoptosis in HCT116 colon carcinoma cells. Apoptosis 12, 411-421.   DOI
13 Min, H., Park, E., Hong, J., Kang, Y., Kim, S., Chung, H., Woo, E., Hung, T. M., Youn, U. J. and Kim, Y. S. 2008. Antiproliferative effects of dibenzocyclooctadiene lignans isolated from Schisandra chinensis in human cancer cells. Bioorg. Med. Chem. Lett. 18, 523-526.   DOI
14 Park, G. H., Song, H. M. and Jeong, J. B. 2017. Kahweol from coffee induces apoptosis by upregulating activating transcription factor 3 in human colorectal cancer cells. Biomol. Ther. (Seoul). 25, 337-343.   DOI
15 Suh, W. S., Park, S. Y., Min, B. S., Kim, S. H., Song, J. H. and Shim, S. H. 2014. The anti-proliferative effects of compounds isolated from Schisandra chinensis. Kor. J. Food Sci. Technol. 46, 665-670.   DOI
16 Saul, R. B., Javier, F., Ignacio, G. R., Claudio, J. V. and Felipe, L. 2017. New Insights toward colorectal cancer chemotherapy using natural bioactive compounds. Front. Pharmacol. 8, 1-22.
17 Seung, J. B., Leigh, C. W. and Thomas, E. E. 2002. Resveratrol enhances the expression of non-steroidal anti-inflammatory drug-activated gene (NAG-1) by increasing the expression of p53. Carcinogenesis 23, 425-434.   DOI
18 Shin, E. J., Chung, S. W. and Hwang, J. T., 2017. Effect of $\gamma$-oryzanol on proliferation and apoptosis of AGS human gastric carcinoma cell. KSBBJ 32, 83-89.   DOI
19 Sun, S. Y., Hail, N. Jr. and Lotan, R. 2004. Apoptosis as a novel target for cancer chemoprevention. J. Natl. Cancer Inst. 96, 662-672.   DOI
20 Wilson, L. C., Baek, S. J., Call, A. and Eling, T. E. 2003. Nonsteroidal anti‐inflammatory drug‐activated gene (NAG‐1) is induced by genistein through the expression of p53 in colorectal cancer cells. Int. J. Cancer 105, 747-753.   DOI
21 Yang, M. H., Kim, J., Khan, I. A., Walker, L. A. and Khan, S. I. 2014. Nonsteroidal anti-inflammatory drug activated gene-1 (NAG-1) modulators from natural products as anti-cancer agents. Life Sci. 100, 75-84.   DOI
22 Yi, H., Chen, Y., Liu, J., Zhang, J., Guo, W., Xiao, W. and Yao, Y. 2016. Extraction and separation of active Ingredients in Schisandra chinensis (Turcz.) Baill and the study of their antifungal effects. PLoS One 11, e0154731.   DOI
23 Yoon, Y. S., Yu, C. S., Jung, S. H., Choi, P. W., Han, K. R., Kim, H. C. and Kim, J. C. 2007. Characteristics of colorectal cancer detected at the health promotion center. J. Kor. Soc. Coloproctol. 23, 321-326.   DOI