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http://dx.doi.org/10.5352/JLS.2018.28.2.187

Blood Pressure Reduction of SHR Treated with EA-contaning Lentinus edodes Mycelial Solid Culture Extract  

Moon, Yeon-Kyu (Gyeongnam Biological Resource Research Center, Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology)
Jung, Jae Eun (Antiaging Institute, HK Biotech., Co., Ltd.)
Cho, Bok-Im (Gyeongnam Biological Resource Research Center, Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology)
Heo, Jeong-Doo (Gyeongnam Biological Resource Research Center, Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology)
Choi, Jungil (Gyeongnam Biological Resource Research Center, Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology)
Kim, Jeong Ok (Antiaging Institute, HK Biotech., Co., Ltd.)
Ha, Yeong Lae (Antiaging Institute, HK Biotech., Co., Ltd.)
Publication Information
Journal of Life Science / v.28, no.2, 2018 , pp. 187-194 More about this Journal
Abstract
Eritadenine (EA), derived from Lentinus edodes (LE), reduced low-density lipoprotein (LDL), triglyceride (TG), and phospholipids in bloods, and fatty acid depositions in animals and humans. Previously, we reported that EA inhibited angiotensin-converting enzyme (ACE) activity in vitro. Now, we report that EA reduced blood pressures in spontaneous hypertension rats (SHR). EA-containing LE mycelial culture enzyme extract (EA-LEMSCEE) was prepared from LE mycelial solid cultures and the hot-water extract of LE fruit bodies. Both EA and EA-LEMSCEE inhibited ACE activity in immortalized human umbilical endothelial cells (EA.hy926). EA-LEMSCEE treatments (7.5 mg/kg, 22.5 mg/kg) significantly reduced systolic and diastolic blood pressure in SHR. At five weeks of treatment, EA-LEMSCEE treatment significantly reduced systolic and diastolic blood pressure, similar to the positive control (captopril, CP; 4 mg/kg) treatment. In addition, the LEMSCEE without EA decreased systolic and diastolic blood pressures compared to the control, but not significant. EA-LEMSCEE decreased renin and ACE activities, and angiotensin II (Ang II) contents in SHR compared to the control. After five weeks of treatment, the effect of EA-LEMCEE was similar to that of CP. These results indicate that EA and EA-LEMSCEE reduce blood pressure by inhibiting the renin and ACE activity of SHR. Furthermore, these results imply that EA or EA-LEMSCEE could be used as an antihypertension agent in humans.
Keywords
Angiotensin converting enzyme (ACE); angiotensin II; eritadenine (EA); Lentinus edodes; renin;
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