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http://dx.doi.org/10.5352/JLS.2018.28.1.110

Effects of Methanol Extracts from Diospyros malabarica Stems on Growth and Biofilm Formation of Oral Bacteria  

Kim, Hye Soo (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Kwon, Hyun Sook (National Development Institute of Korean Medicine)
Kim, Chul Hwan (Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources)
Lee, Sang Woo (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology)
Sydara, Kongmany (Institute of Traditional Medicine, Ministry of Health, Vientiane Capital)
Cho, Soo Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Life Science / v.28, no.1, 2018 , pp. 110-115 More about this Journal
Abstract
This study was conducted to investigate the potential of medicinal plants as oral health materials derived from natural products. Among the extracts from 200 medicinal plants grown in Nepal, Laos, Mongolia, Bangladesh, Vietnam, and China, stem extracts from Diospyros malabarica (1 mg/disc) showed the highest antibacterial activity against Porphyromonas gingivalis ATCC33277 and Streptococcus mutans ATCC25175. The D. malabarica stem extracts showed antibacterial activity similar to chlorhexidine, sodium lauryl sulfate, and triclosan, which were used as a positive control, as well as higher antibacterial activity against S. mutans ATCC25175 than P. gingivalis ATCC33277. The D. malabarica stem extracts showed bactericidal action (MBC, 0.4 mg/ml) against P. gingivalis ATCC33277 and bacteriostatic action against S. mutans ATCC25175. The biofilm production rate of S. mutans ATCC25175 and the expression of the comX gene associated to biofilm formation in the cultures treated with 0.2-1.0 mg/ml of D. malabarica stem extracts were suppressed in a concentration-dependent manner. Based on the above results, it can be concluded that D. malabarica stem extracts can be used as oral health material derived from natural materials, as demonstrated by the bacteriostatic action and inhibition of biofilm formation against S. mutans ATCC25175.
Keywords
Biofilm formation; comX; Diospyros malabarica; P. gingivalis; S. mutans;
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1 Pranjal, S. and Debabrat, B. 2014. Phytochemical analysis and antioxidant activity of Gardenia jasminoides ellis and Diospyros malabarica kostel. Int. J. Pharm. Bio. Sci. 5, 199-204.
2 Pratt, L. A. and Kolter, R. 1999. Genetic analyses of bacterial biofilm formation. Curr. Opin. Microbiol. 2, 598-603.   DOI
3 Ramsewak, R. S., Nair, M. G., Stommel, M. and Selanders, L. 2003. In vitro antagonistic activity of monoterpenes and their mixtures against 'toe nail fungus' pathogens. Phytother. Res. 17, 376-379.   DOI
4 Adolfsson, E. M., Pettersson, M., Parkkonen, J. and Sturve, J. 2002. Triclosan, a commonly used bactericide found in human milk and in the aquatic environment in Sweden. Chemosphere 46, 1485-1489.   DOI
5 Bakaletz, L. O. 2004. Developing animal models for polymicrobial diseases. Nat. Rev. Microbiol. 2, 552-568.   DOI
6 Burne, R. A. 1998. Oral Streptococci products of their environment. J. Dent. Res. 77, 445-452.   DOI
7 Davidson, P. M. and Parish, M. E. 1989. Methods for testing the efficacy of food antimicrobials. Food Technol. 43, 148-152.
8 Cramton, S. E., Gerke, C., Schnell, N. F., Nichols, W. W. and Gotz, F. 1999. The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infect. Immun. 67, 5427-5433.
9 Chen, C. P., Lin, C. C. and Tsuneo, N. 1989. Screening of Taiwanese crude drugs for antibacterial activity against Streptococcus mutans. J. Ethnopharmacol. 27, 285-295.   DOI
10 Choi, M. S. and Ahn, K. S. 2014. Antibacterial effect of bamboo charcoal on Streptococcus mutans. J. Kor. Soc. Dent. Hyg. 14, 95-100.   DOI
11 Huh, M. K. and Kim, H. J. 2014. Antibacterial effect on leaf-extract from Nelumbo nucifera against oral microorganism. J. Kor. Soc. Dent. Hyg. 14, 117-122.   DOI
12 Iauk, L., Lo Bue, A. M., Milazzo, I., Rapisarda, A. and Blandino, G. 2003. Antibacterial activity of medicinal plant extracts against periodontopathic bacteria. Phytother. Res. 17, 599-604.   DOI
13 Kim, H. E. 2014. Change of paradigms in caries-associated bacteria in the caries process: ecological perspectives. J. Dent. Hyg. Sci. 14, 87-93.
14 Kim, S. K., Shin, M. K., Auh, Q. S., Lee, J. Y., Hong, J. P. and Chun, Y. H. 2007. Effect of phytoncide on Porphyromonas gingivalis. J. Oral. Med. Pain. 32, 137-150.
15 Kumar, S., Shukla, Y. N., Lavania, U. C., Sharma, A. and Singh, A. K. 1997. Medicinal and aromatic plants: prospects for India. J. Med. Arom. Pl. Sc. 19, 361-365.
16 Stoodly, P., Sauer, K., Davies, D. G. and Costerton, J. W. 2002. Biofilms as complex differentiated communities. Annu. Rev. Microbiol. 56, 187-209.   DOI
17 Kuramitsu, H. K., He, X., Lux, R., Anderson, M. H. and Shi, W. 2007. Interspecies interactions within oral microbial communities. Microbiol. Mol. Biol. Rev. 71, 653-670.   DOI
18 Lee, D. H., Yu, H. H., Jung, S. Y., Moon, H. D., Kim, S. M., Jeon, B. H. and You, Y. O. 2007. Anticariogenic preoperties of the ethanol extract of Tribuli fructus against Streptococcus mutans. J. Physiol. Pathol. Kor. Med. 21, 1148-1153.
19 Lee, Y. S., Jang, K. A. and Cha, J. D. 2012. Synergistic anti bacterial effect between silibinin and antibiotics in oral bacteria. J. Biomed. Biotechnol. 2012, 1-7.
20 Rule, K. L., Ebbett, V. R. and Vikesland, P. J. 2005. Formation of chloroform and chlorinated organics by free-chlorinemediated oxidation of triclosan. Environ. Sci. Technol. 39, 3176-3185.   DOI
21 Stepanovic, S., Vukovic, D., Hola, V., Bonaventura, G., Djukic, S., Cirkovic, I. and Ruzicka, F. 2007. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by Staphylococci. APMIS. 115, 891-899.   DOI
22 Stewart, P. S. and Costerton, J. W. 2001. Antibiotic resistance of bacteria in biofilms. Lancet 358, 135-138.   DOI
23 Zhou, L., Ding, Y., Chen, W., Zhang, P., Chen, Y. and Lv, X. 2013. The in vitro study of ursolic acid and oleanolic acid inhibiting cariogenic microorganisms as well as biofilm. Oral Dis. 19, 494-500.   DOI
24 Mahesh, S. R., Mohan, G. K., Namdev, Y. G. and Sanjay, J. S. 2013. Evaluation of anti-diarrheal activity of Diospyros malabarica bark extract. Bangladesh J. Pharmacol. 8, 49-53.
25 Li, Y. H., Tang, N., Aspiras, M. B., Lau, P. C. Y., Lee, J. H., Ellen, R. P. and Cvitkovitch, D. G. 2002. A quorum-sensing signaling system essential for genetic competence in Streptococcus mutans is involved in biofilm formation. J. Bacteriol. 184, 2699-2708.   DOI
26 Lin, M., Savaiano, D. and Harlande, S. 1991. Influence of nonfermented dairy products containing bacterial stater cultures on lactose maldigestion in humans. J. Dairy Sci. 74, 87-95.   DOI
27 Loo, C. Y., Corliss, D. A. and Ganeshkumar, N. 2000. Streptococcus gordonii biofilm formation: identification of genes that code for biofilm phenotypes. J. Bacteriol. 182, 1374-1382.   DOI
28 Park, K. M., You, J. S., Lee, H. Y., Baek, N. I. and Hwang, J. K. 2003. Kuwanon G: an antibacterial agent from the root bark of Morus alba against oral pathogens. J. Ethnophpharmacol. 84, 181-185.   DOI
29 Poureslami, H. 2012. The effects of plant extracts on dental plague and caries. MY.(ed.). Croatia: In tech. 2012, 96-402.