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http://dx.doi.org/10.5352/JLS.2018.28.10.1156

Anti-thrombotic Activities of Hot-water Extracts Prepared from Various Parts of Nelumbo nucifera Gaertner  

Ahn, Seon-Mi (Department of Food and Nutrition, Andong National University)
Sung, Hwa-Jung (Department of Food and Nutrition, Andong National University)
Kim, Jong-Sik (Department of Biological Science, Andong National University)
Park, Jong-Yi (Gyeongbuk Institute For Bio-Industry)
Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
Publication Information
Journal of Life Science / v.28, no.10, 2018 , pp. 1156-1162 More about this Journal
Abstract
To investigate the anti-thrombotic activities of the lotus (Nelumbo nucifera Gaertner), various hot-water extracts were prepared from the leaf, pod of seed (PS), seed, embryo of seed (ES), root, and the node of root (NR) of the lotus. The highest extraction ratio was found in the NR (20.3%), followed by the seed, root, leaf, ES, and PS. These extracts had pH and acidity levels ranging from 5.6~6.5 and 0.06~0.20%, respectively. The seed extract showed 70% brix, whereas the leaf and PS extracts showed less than 0.1% brix. The highest contents of total polyphenol (179.7 mg/g), total flavonoids (161.4 mg/g), and reducing sugar (161.4 mg/g) were observed in the leaf extract, and the highest total sugar content (873.0 mg/g) in the seed extract. Anti-coagulation assay of the extracts of NR, leaf, and PS showed strong activities. In particular, at a concentration of 5 mg/ml, the PS extract had 15-fold extended thrombin, prothrombin, and activated partial thromboplastin times. However, only the ES extract showed activities inhibitory to platelet aggregation, with treatment with 0.25 mg/ml of ES extract decreasing platelet aggregation to 25.1%, a reduction comparable to that effected by aspirin. The extracts other than the seed extract showed no hemolysis activities against human RBC at treatments of up to 1 mg/ml. These results suggest that the NR, PS, seed, and ES, all byproducts of the lotus agriculture industry, have high potential as novel sources of anti-thrombotic agent.
Keywords
Anticoagulation; anti-platelet aggregation; Nelumbo nucifera Gaertner; node of root; pod of seed;
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