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http://dx.doi.org/10.5352/JLS.2017.27.9.1031

Hepatoprotective Effect of Bacillus subtilis-fermented Silkworm (Bombyx mori L.) Extract on Non-alcoholic Fatty Liver in Rats  

Kim, Tae-Hoon (Department of Biotechnology, Dong-A University)
Ahn, Hee-Young (Department of Biotechnology, Dong-A University)
Kim, Young-Wan (Department of Biotechnology, Dong-A University)
Sim, So-Yeon (Department of Biotechnology, Dong-A University)
Cho, Hyun-Dong (Department of Food Science and Biotechnology, Kyungpook National University)
Kim, Man-Do (GSP Co., Ltd.)
Lee, You-Jung (GSP Co., Ltd.)
Cho, Young-Su (Department of Biotechnology, Dong-A University)
Publication Information
Journal of Life Science / v.27, no.9, 2017 , pp. 1031-1039 More about this Journal
Abstract
The aim of this study was to investigate the potential effects of extracts from silkworm Bombyx mori L. fermented with Bacillus subtilis KACC 91157 at levels of 5%(v/w) and 10%(v/w) in Sprague-Dawley rats intoxicated with 1%(w/w) orotic acid (OA) for 10 days. The rats were divided into a normal group (N), a control group (C: OA), and treatment groups (SP10: OA + 10% extracts from B. mori L.; BSP5: OA + 5% extracts from B. mori L. fermented with B. subtilis KACC 91157; BSP10: OA + 10% extracts from B. mori L. fermented with B. subtilis KACC 91157). Serum activities of aspartate aminotransferase (AST), alanine transferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) increased following OA feeding, but the rise was slightly reduced by administration of BSP10. The total lipid, free fatty acid, phospholipid, total cholesterol, and triglyceride contents in serum were significantly lower in the OA treatment groups than in the N group. However, the contents slightly increased following the administration of BSP10. Glutathione concentrations in liver and serum were reduced in the OA-induced fatty liver, but they increased following the administration of BSP10. Hepatocytes in the OA-induced fatty liver contained numerous large droplets. However, SP10, BSP5, and BSP10 feeding prevented OA-induced lipid droplet accumulation in hepatocytes. Accordingly, extracts from silkworm powder fermented with B. subtilis could be an ideal material as a dietary supplement in healthy functional foods to improve the effects of fatty liver.
Keywords
Bacillus subtilis; bioconversion; Bombyx mori L.; fatty liver; fermentation; orotic acid;
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