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http://dx.doi.org/10.5352/JLS.2017.27.7.796

Identification of Antioxidant Compound Derived from Methanolic Extract of Houttuynia Cordata  

Kim, Hyeji (Department of Chemistry, Dong-Eui University)
Hwang, Heesung (Department of Chemistry, Dong-Eui University)
Park, Sumin (Department of Chemistry, Dong-Eui University)
Kang, Sungwook (Department of Chemistry, Dong-Eui University)
Kim, Hyejeong (Department of Chemistry, Dong-Eui University)
Hong, Sugyeong (Department of Chemistry & Biology, Dong-Eui University)
Kim, Moon-Moo (Department of Applied Chemistry, Dong-Eui University)
Oh, Yunghee (Department of Applied Chemistry, Dong-Eui University)
Publication Information
Journal of Life Science / v.27, no.7, 2017 , pp. 796-804 More about this Journal
Abstract
This study was carried out to evaluate the antioxidant effect of methanolic extract of Houttuynia cordata (HCME) and to identify a compound having antioxidant effect. The ethyl acetate fraction of HCME showed the highest antioxidant effect in organic solvent fractions. The fraction was then separated into 12 fractions by open column chromatography. Among these fractions, the fraction 10 (Fr. 10) with the highest antioxidant activity was isolated, and its antioxidant effect was evaluated by DPPH radical scavenging activity, reducing power, TBARS, cell viability, DNA oxidation and DCF fluorescence. The Fr. 10 at a $64{\mu}g/ml$ showed 60% of inhibitory effect similar to that of vitamin C at $10{\mu}g/ml$, compared with blank group. The Fr. 10 at $64{\mu}g/ml$ showed 264% of reducing power, compared with blank group. TBARS assay showed that the Fr. 10 at $64{\mu}g/ml$ had 35.5% of inhibitory effect similar to that of vitamin E at $1,000{\mu}g/ml$, compared with blank group. The Fr. 10 above $32{\mu}g/ml$ displayed cytotoxicity. However, it was observed that the Fr. 10, above $1{\mu}g/ml$ reduced DNA damage. DCF fluorescence assay showed that the Fr. 10 inhibited oxidative stress by $H_2O_2$ in a dose dependent manner. The compound of Fr. 10 was identified to be rutin whose molecular weight is 610 by the IR and LC-MS analyses. Therefore, these results suggest that the rutin of Fr. 10 could use as a natural antioxidant for development of cosmetics and functional foods.
Keywords
Antioxidant effect; DCF fluorescence; Houttuynia cordata; rutin;
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