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http://dx.doi.org/10.5352/JLS.2017.27.7.739

Expression and Localization of 20α-Hydroxysteroid Dehydrogenase in Immature Pig Testis  

Kim, Jeong-Soo (Animal Biotechnology, Graduate School of Future Convergence Technology, Department of Animal Resource Science, Institute of Genetic Engineering, Hankyong National University)
Seong, Hun-Ki (Animal Biotechnology, Graduate School of Future Convergence Technology, Department of Animal Resource Science, Institute of Genetic Engineering, Hankyong National University)
Byambaragchaa, Munkhzaya (Animal Biotechnology, Graduate School of Future Convergence Technology, Department of Animal Resource Science, Institute of Genetic Engineering, Hankyong National University)
Sim, Bo-Woong (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology)
Her, Chang-Gi (MediKinetics)
Kang, Myung-Hwa (Department of Food Science and Nutrition, Hoseo University)
Min, Kwan-Sik (Animal Biotechnology, Graduate School of Future Convergence Technology, Department of Animal Resource Science, Institute of Genetic Engineering, Hankyong National University)
Publication Information
Journal of Life Science / v.27, no.7, 2017 , pp. 739-745 More about this Journal
Abstract
In all mammalian species, progesterone is essential in the preparation for and maintenance of pregnancy. $20{\alpha}$-hydroxysteroid dehydrogenase ($20{\alpha}$-HSD) predominantly converts progesterone into its biologically inactive form $20{\alpha}$-hydroxyprogesterone ($20{\alpha}$-OHP), and plays a crucial role in the termination of pregnancy and initiation of parturition. In this study, we characterized the expression and localization of $20{\alpha}$-HSDinthe testis of MediKinetics $Micropigs^{(R)}$. The testes were collected at days 6, 9, 12, 18, and 21 after birth. The $20{\alpha}$-HSD mRNA was found to be expressed in the testis at day 6 after birth by RT-PCR. The highest level of mRNA expression in the testis was detected on day 21 after birth. However, the mRNA was not detected in the placenta after parturition. Western blot for $20{\alpha}$-HSD reveal that the specific 37-kDa band was detected in immature pig testis. However, this band was not detected in testis tissue at day 6 after birth. In the immunohistochemical analysis of the testis, $20{\alpha}$-HSD was detected in the Sertoli cells and Leydig cells. Taken together, our study shows for the first time that the $20{\alpha}$-HSD mRNA and protein are expressed in pig testis after birth. Further investigation is required to elucidate the functional mechanisms of $20{\alpha}$-HSD in pig testis after birth.
Keywords
$20{\alpha}$-HSD; expression; IHC; miniature pig; testis;
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