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http://dx.doi.org/10.5352/JLS.2017.27.12.1415

Molecular Cloning and Expression of the Metallothionein Gene under Environmental Stresses in Sweet Potato  

Kim, Young-Hwa (Ubiquitous Healthcare Research Center, College of Liberal Arts, Inje University)
Yu, Eun Jeong (Institute of Digital Anti-Aging Healthcare, Graduate School of Inje University)
Huh, Gyung-Hye (Ubiquitous Healthcare Research Center, College of Liberal Arts, Inje University)
Publication Information
Journal of Life Science / v.27, no.12, 2017 , pp. 1415-1420 More about this Journal
Abstract
The metallothionein (MT) gene (IbMT3) was selected from an EST library of suspension-cultured sweet potato cells. The MT gene, which is one of abundant ESTs in the library, is involved in stress regulation of cells and tissues. A full-length IbMT3 cDNA was obtained and analysis of its nucleotide sequence revealed that IbMT3 encoded a type 3 MT protein, based on its structural characteristics. The function of type 3 MT in plants is not yet known. Northern blot analysis showed stronger expression of IbMT3 in suspension-cultured cells than in sweet potato plant leaves. Since cell culture is known to impose a state of oxidative stress on cells, sweet potato plants were subjected to oxidative stress to investigate the transcriptional regulation of IbMT3. When the herbicide methyl viologen (MV) was administered for 6, 12, and 24 hr, IbMT3 transcription rapidly increased at 6 hr and then decreased. A cold treatment at $15^{\circ}C$ for 24 and 48 hr resulted in a gradual increase in IbMT3 expression. These findings indicate that IbMT3 expression is regulated in response to environmental and oxidative stress. IbMT3 isoform is expected to have antioxidant effects in sweet potato plants and may play an important role in cellular adaptation to oxidative stress.
Keywords
Antioxidant; metallothionein; oxidative stress; sweet potato;
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