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http://dx.doi.org/10.5352/JLS.2017.27.10.1207

Inhibitory Effects of Schisandrae Fructus Ethanol Extract on the Production of Matrix Metalloproteinases in in vitro and in vivo Osteoarthritis Models  

Jeong, Jin-Woo (Open Laboratory for Muscular & Skeletal Disease Control, and Department of Biochemistry Dongeui University College of Korean Medicine)
Lee, Hye Hyeon (Open Laboratory for Muscular & Skeletal Disease Control, and Department of Biochemistry Dongeui University College of Korean Medicine)
Kim, Hong Jae (Open Laboratory for Muscular & Skeletal Disease Control, and Department of Biochemistry Dongeui University College of Korean Medicine)
Lee, Ki Won (Research Institute, Bio-Port Korea INC, Marine Bio-industry Development Center)
Kim, Ki Young (Research Institute, Bio-Port Korea INC, Marine Bio-industry Development Center)
Kim, Sung Goo (Research Institute, Bio-Port Korea INC, Marine Bio-industry Development Center)
Hong, Su-Hyun (Open Laboratory for Muscular & Skeletal Disease Control, and Department of Biochemistry Dongeui University College of Korean Medicine)
Kim, Bum Hoi (Department of Anatomy, Dongeui University College of Korean Medicine)
Park, Cheol (Department of Molecular Biology, College of Natural Sciences, Dongeui University)
Choi, Yung Hyun (Open Laboratory for Muscular & Skeletal Disease Control, and Department of Biochemistry Dongeui University College of Korean Medicine)
Publication Information
Journal of Life Science / v.27, no.10, 2017 , pp. 1207-1214 More about this Journal
Abstract
Schisandrae Fructus (SF), the fruit of Schisandra chinensis (Turcz.) Baill., is widely used in traditional medicine for the treatment of a number of chronic diseases. SF extracts have been recently reported to attenuate the inflammatory responses in SW1353 human chondrocyte cells in in vitro and monosodium iodoacetate (MIA)-induced cartilage degradation in in vivo osteoarthritis (OA) models. However, their protective and therapeutic potentials against OA in primary culture chondrocytes and animal models remain unclear. Therefore, we investigated the effects of the ethanol extract of SF on the activity of matrix metalloproteinases (MMPs), biomarkers for diagnosis of OA, on interleukin $(IL)-1{\beta}-induced$ primary cultured rat cartilage chondrocytes and MIA-induced osteoarthritis in a rat model. Our data indicated that SF treatment significantly reduced the mRNA expression and enzyme activity of MMP-1, -3 and -13 in $IL-1{\beta}-induced$ primary cultured rat cartilage chondrocytes. The chondro-protective effects of SF were then analyzed in a rat OA model using a single intra-articular injection of MIA in the right knee joint. According to our results, the elevated levels of MMP-1 and -3 were markedly ameliorated by SF administration. Collectively, these findings indicate that SF could be a candidate for the treatment of OA.
Keywords
$Interleukin-1{\beta}$; matrix metalloproteinase; monosodium iodoacetate; osteoarthritis; Schisandrae Fructus (SF);
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