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http://dx.doi.org/10.5352/JLS.2017.27.10.1097

Induction of Sexual Maturation in Female Eels (Anguilla japonica) Using an Osmotic Pump  

Kim, Jung-Hyun (Aquaculture Research Division, National Institute of Fisheries Science)
Kim, Dae-Geun (Aquaculture Research Division, National Institute of Fisheries Science)
Kim, Hyo-Won (Aquaculture Research Division, National Institute of Fisheries Science)
Lee, Bae-Ik (Aquaculture Research Division, National Institute of Fisheries Science)
Kim, Shin-Kwon (Aquaculture Research Division, National Institute of Fisheries Science)
Jun, Je-cheon (Aquaculture Research Division, National Institute of Fisheries Science)
Myeong, Jeong-In (Aquaculture Research Division, National Institute of Fisheries Science)
Kim, Dae-Jung (Aquaculture Research Division, National Institute of Fisheries Science)
Publication Information
Journal of Life Science / v.27, no.10, 2017 , pp. 1097-1103 More about this Journal
Abstract
For the artificial induction of the sexual maturation of Anguilla japonica, salmon pituitary extract (SPE) is continuously injected into females, and the eggs obtained from artificial sexual maturation are artificially fertilized with sperms and hatched. However, repeated injection of SPE in the abdominal cavity causes tremendous stress in females, which may prevent their complete sexual maturation and reduce the immune system function, ultimately resulting in death. In addition, the poor quality of the ovulated eggs can reduce the hatching and survival rate of larvae. In the present study, sexual maturation of females was induced by inserting an osmotic (OS) pump containing hormone analogs known to effectively induce sexual maturation into the abdominal cavity of female eels, and the effect of the OS pump on the induced sexual maturation was investigated. Our study results showed that the gonadosomatic index (GSI) was significantly higher in the fish subjected to SPE injection than those subjected to human chorionic gonadotropin (hCG), gonadotropin-releasing hormone analog (GnRHa), and methyl testosterone (MT) injections, either separately or in combination. In addition, a histological analysis showed that the oocytes in the SPE OS pump groups were more mature (entered the nuclear shift stage) than those in the other groups. These results suggest that an osmotic pump containing hormone analogs can be used to induce sexual maturation in female A. japonica artificially.
Keywords
Eel; gonadal development; osmotic pump; reproduction;
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1 Kagawa, H., Fujie, N., Imaizumi, H., Masuda, Y., Oda, K., Adachi, J. and Kaji, S. 2013. Using osmotic pumps to deliver hormones to induce sexual maturation of female Japanese eels, Anguilla japonica. Aquaculture 388, 30-34.
2 Kim, D. J., Kang, E. J., Bae, J. Y., Park, M. W. and Kim, E. O. 2007. Development of the eggs and pre-leptocephalus Larvae by natural spawning of artificially-matured Japanese eel, Anguilla japonica. J. Aquacult. 20, 160-167.
3 Kim, D. J., Lee, B. I., Kim, K. K., Kim, E. O., Son, M. H. and Seong, K. B. 2013. Effects of estradiol-$17{\beta}$ on the feminization of Japanase eel, Anguilla japonica. J. Life Sci. 23, 998-1003.   DOI
4 Kim, D. J., Kim, Y. C., Choi, Y. K., Son, M. H., Lee, J. U., Park, M. S. and Heo, Y. S. 2009. Effects of rearing condition during the winter period on artificial maturation and reproduction of cultured female eel, Anguilla japonica. Dev. Reprod. 13, 35-41.
5 Kim, D. J. and Aida, K. 2000. Dopaminergic regulation of Gonadotropin-II secretion in testosterone-treated precocious male and immature rainbow trout oncorhynchus mykiss. Kor. J. Biol. Sci. 4, 287-292.   DOI
6 Moussavi, M., Nelson, E. R. and Habibi, H. R. 2009. Seasonal regulation of vitellogenin by growth hormone in the goldfish liver. Gen. Comp. Endocrinol. 161, 79-82.   DOI
7 Mylonas, C. C. and Zohar, Y. 2000. Use of GnRHa-delivery systems for the control of reproduction in fish. Rev. Fish Biol. Fish. 10, 463-491.   DOI
8 Nagae, M., Todo, T., Gen, K., Kato, Y., Young, G., Adachi, S. and Yamauchi, K. 1996. Molecular cloning of the cDNAs encoding pituitary glycoprotein hormone ${\alpha}$-and gonadotropin $II{\beta}$-subunits of the Japanese eel, Anguilla japonica, and increase in their mRNAs during ovarian development induced by injection of chum salmon pituitary homogenate. J. Mol. Endocrinol. 16, 171-181.   DOI
9 Ohta, H., Kagawa, H., Tanaka, H., Okuzawa, K. and Hirose, K. 1996. Milt production in the Japanese eel Anguilla japonica induced by repeated injections of human chorionic gonadotropin. Fish. Sci. 62, 44-49.   DOI
10 Ohta, H., Kagawa, H., Tanaka, H., Okuzawa, K. and Hirose, K. 1996. Changes in fertilization and hatching rates with time after ovulation induced by 17, $20{\beta}$-dihydroxy-4-pregnen-3-one in the Japanese eel, Anguilla japonica. Aquaculture 139, 291-301.   DOI
11 Patton, P. E., Stouffer, R. L. and Zelinski-Wooten, M. B. 1990. The effect of intraluteal infusion of deglycosylated human chorionic gonadotropin on the corpus luteum in rhesus monkeys. J. Clin. Endocrinol. Metab. 70, 1213-1218.   DOI
12 Rocha, A., Gomez, A., Galay-Burgos, M., Zanuy, S., Sweeney, G. E. and Carrillo, M. 2007. Molecular characterization and seasonal changes in gonadal expression of a thyrotropin receptor in the European sea bass. Gen. Comp. Endocrinol. 152, 89-101.   DOI
13 Sato, N., Kawazoel, I., Suzuki, Y. and Aida, K. 2003. Induction of vitellogenesis. In Eel Biology (pp. 387-399).
14 Sato, N., Kawazoe, I., Shiina, Y., Furukawa, K., Suzuki, Y. and Aida, K. 1995. A novel method of hormone administration for inducing gonadal maturation in fish. Aquaculture 135, 51-58.   DOI
15 Tamaru, C. S., Kelley, C. D., Lee, C. S., Aida, K. and Hanyu, I. 1989. Effects of chronic LHRH-a+ 17-methyltestosterone or LHRH-a+ testosterone therapy on oocyte growth in the striped mullet (Mugil cephalus). Gen. Comp. Endocrinol. 76, 114-127.   DOI
16 Crim, L. W., Sutterlin, A. M., Evans, D. M. and Weil, C. 1983. Accelerated ovulation by pelleted LHRH analogue treatment of spring-spawning rainbow trout (Salmo gairdneri) held at low temperature. Aquaculture 35, 299-307.   DOI
17 Unuma, T., Kondo, S., Tanaka, H., Kagawa, H., Nomura, K. and Ohta, H. 2004. Determination of the rates of fertilization, hatching and larval survival in the Japanese eel, Anguilla japonica, using tissue culture microplates. Aquaculture 241, 345-356.   DOI
18 Yamamoto, K., Oomori, M. and Yamauchi, K. 1974. Oogenesis of the Japanese eel. Nippon Suisan Gakkaishi. 40, 9-15.   DOI
19 Aida, K. 1983. Effect of LH-releasing hormone on gonadal develop mentin salmonid fish, the Ayu. Bull. Jpn. Soc. Sci. Fish. 49, 711-718.   DOI
20 Bae, J. Y., Kim, D. J., Lee, J. U., Son, S. G. and Lee, J. K. 2007. Correlationship between artificial maturation season and reproduction coefficient in the cultured eel Anguilla japonica. J. Aquacult. 20, 219-225.
21 Kagawa, H., Kasuga, Y., Adachi, J., Nishi, A., Hashimoto, H., Imaizumi, H. and Kaji, S. 2009. Effects of continuous administration of human chorionic gonadotropin, salmon pituitary extract, and gonadotropin-releasing hormone using osmotic pumps on induction of sexual maturation in male Japanese eel, Anguilla japonica. Aquaculture 296, 117-122.   DOI
22 Zohar, Y. and Mylonas, C. C. 2001. Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture 197, 99-136.   DOI
23 Dufour, S., Bassompierre, M., Montero, M., Belle, N. L., Baloche, S. and Fontaine, Y. A. 1991. Stimulation of pituitary gonadotropic function in female silver eel treated by a gonadoliberin agonist and dopamine antagonists. In: Proc. The Fourth International Symposium on the Reproductive Physiology of Fish, pp. 54-56. Edited by A.P. Scott, J.P. Sumpter, D.E. Kime and M.S. Rolfe. Fish Symp 91, Sheffield, UK.
24 Dufour, S., Burzawa-Gerard, E., Le Belle, N., Sbaihi, M. and Vidal, B. 2003. Reproductive endocrinology of the European eel, Anguilla anguilla. In Eel biology (pp. 373-383).
25 Gibson, M. J., Kasowski, H. and Dobrjansky, A. 1994. Continuous gonadotropin-releasing hormone infusion stimulates dramatic gonadal development in hypogonadal female mice. Biol. Reprod. 50, 680-685.   DOI
26 Girling, J. E., Bennett, E. J. and Cockrem, J. F. 2002. Administration of pregnant mare serum gonadotropin to Japanese quail (Coturnix coturnix japonica): dose response over seven days and comparison of delivery by daily injection or osmotic pump. N. Z. Vet. J. 50, 115-121.
27 Ijiri, S., Kayaba, T., Takeda, N., Tachiki, H., Adachi, S. and Yamauchi, K. 1998. Pretreatment reproductive stage and oocyte development induced by salmon pituitary homogenate in the Japanese eel Anguilla japonica. Fish. Sci. 64, 531-537.   DOI
28 Kagawa, H., Tanaka, H., Ohta, H., Unuma, T. and Nomura, K. 2005. The first success of glass eel production in the world: basic biology on fish reproduction advances new applied technology in aquaculture. Fish Physiol. Biochem. 31, 193-199.   DOI