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http://dx.doi.org/10.5352/JLS.2016.26.6.640

Green Tea Polyphenol Epigallocatechine Gallate (EGCG) Prevented LPS-induced BV-2 Micoglial Cell Activation  

Park, Euteum (Department of Biomedical Science, College of Natural Sciences, Chosun University)
Chun, Hong Sung (Department of Biomedical Science, College of Natural Sciences, Chosun University)
Publication Information
Journal of Life Science / v.26, no.6, 2016 , pp. 640-645 More about this Journal
Abstract
Microglial cells are immediately activated in the central nervous system in response to a variety of neuronal environmental changes, such as injuries or inflammation. In addition to the modulation of the intrinsic immune response, a key role of microglial cells is the phagocytosis of dying cells and cellular debris. In this study, the inhibitory effects of epigallocatechine-3-gallate (EGCG), a most abundant and active polyphenol component of green tea, on lipopolysaccharide (LPS)-induced microglial activation are determined. EGCG dose dependently suppressed LPS-induced nitric oxide production and the expression of inducible nitric oxide synthase (iNOS) in BV-2 microglial cells. EGCG are potent LPS-induced inhibitors of several pro-inflammatory cytokine expressions, such as TNF-α and IL-1β, in microglial cells. Furthermore, EGCG generally inhibits the induction of LPS-mediated microglial activation and potently inhibits the phagocytosis of LPS-stimulated BV2 microglia. Although the conditioned media from LPS-stimulated BV-2 cells caused the SN4741 cell death, that from the conditioned media of EGCG pretreated BV-2 cells did not diminish the viability of SN4741 cells. These results suggest EGCG, a green tea polyphenol, could be a promising available molecule for the modulation of harmful microglial activation.
Keywords
Cytokine; EGCG (epigallocatechine-3-gallate); inflammation; microglia; phagocytosis;
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