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http://dx.doi.org/10.5352/JLS.2016.26.5.509

Effects of an Aqueous Extract of Asparagus cochinchinensis on the Regulation of Nerve Growth Factor in Neuronal Cells  

Lee, Hyun Ah (College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University)
Kim, Ji Eun (College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University)
Song, Sung Hwa (College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University)
Sung, Ji Eun (College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University)
Jung, Min Gi (College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University)
Kim, Dong Seob (College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University)
Son, Hong Joo (College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University)
Lee, Chung Yeoul (Gangrim Organics)
Lee, Hee Seob (College of Human Ecology, Pusan National University)
Hwang, Dae Youn (College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University)
Publication Information
Journal of Life Science / v.26, no.5, 2016 , pp. 509-518 More about this Journal
Abstract
Asparagus cochinchinensis is a medical plant that has long been used to treat fever, cough, kidney disease, breast cancer, inflammatory disease and brain disease in northeast Asian countries. Although several studies have been conducted on the anti-neuroinflammatory effects of A. cochinchinensis, the correlation between these effects and nerve growth factor (NGF) has not yet been examined. In this study, we investigated the effects of an aqueous extract of A. cochinchinensis (AEAC) on the secretion and action mechanism of NGF in neuronal cells. The concentration of the NGF protein in the supernatant collected from cultured cells increased significantly in B35 cells treated with AEAC in comparison with the vehicle-treated group without any specific cytotoxicity. Furthermore, the mRNA expression of NGF showed a very similar pattern to its protein concentration. To examine the bioactivity of NGF secreted from B35 cells, undifferentiated PC12 cells were cultured in an AEAC-conditioned medium and neuritic outgrowth was observed. The dendrite length of PC12 cells in the AEAC-treated group was significantly higher than that in the vehicle-treated group. Moreover, the level of the downstream effectors p-TrkA and p-ERK of the high-affinity NGF receptor was significantly higher in the AEAC-treated group, while the expression of the downstream effectors of the low-affinity NGF receptor was significantly lower in the same group. These results suggest that AEAC may contribute to the regulation of NGF expression and secretion in neuronal cells; it is therefore an excellent candidate for further investigation as a therapeutic drug for neurodegenerative diseases.
Keywords
Asparagus cochinchinensis; B35 cells; NGF; NGF receptor; PC12 cells;
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