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http://dx.doi.org/10.5352/JLS.2016.26.1.59

Effect of Fermented Platycodon grandiflorum Extract on Cell Proliferation and Migration in Bovine Aortic Endothelial Cells  

Choi, Woosoung (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, CKII undergraduate program of Chembio, Dankook University)
Song, Jina (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, CKII undergraduate program of Chembio, Dankook University)
Park, Mi-Hyeon (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, CKII undergraduate program of Chembio, Dankook University)
Yu, Heui Jong (Health Food R&D Center, Bioland)
Park, Heonyong (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, CKII undergraduate program of Chembio, Dankook University)
Publication Information
Journal of Life Science / v.26, no.1, 2016 , pp. 59-67 More about this Journal
Abstract
Platycodon grandiflorum A. De Candolle (Korean name, ‘Doraji’) is a perennial plant containing various triterpenoid saponins. The roots of this plant have traditionally been used as a food material in Korea. Here, we prepared a fermented P. grandiflorum extract (PG). Although it was previously reported that P. grandiflorum A. extract has a variety of physiological functionalities, including anti-inflammatory and anti-oxidant activities, little is known about its vascular functions. In this study, we executed a series of experiments to identify the effect of PG on endothelial cells. PG at a high concentration (100 μg/ml) was found to induce cell detachment, whereas PG at a low concentration (0.1 μg/ml) appeared to promote cell proliferation and migration in bovine aortic endothelial cells. The cell detachment induced by the high concentration was not associated with cell death, such as apoptosis, necrosis, and autophagy. In addition, we found that PG at the high concentration formed a small vesicular structure called an endothelial microparticle (EMP). The EMP was prepared by centrifugal fractionation and determined with flow cytometry and a microscope. Interestingly, PG-induced cell detachment was found to be mediated by EMP. We furthermore determined that PG at the low concentration activated Akt, a crucial cell-signaling molecule, and then controlled cell proliferation and migration. Overall, our findings suggest that PG at low doses maintains vascular stability by promoting endothelial cell proliferation, and enhances the efficacy of wound healing by cell proliferation and migration activity.
Keywords
Akt; cell proliferation; cell migration; endothelial microparticle; Platycodon grandiflorum A. extract;
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Times Cited By KSCI : 8  (Citation Analysis)
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