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http://dx.doi.org/10.5352/JLS.2016.26.1.117

Suppressive Effects of Lees from Sweet Potato Soju on LPS-induced Inflammatory Responses in RAW 264.7 Cells  

Lee, Seung-Hoon (Department of Biological Sciences, Andong National University)
Kwon, Min-Jeong (Department of Biological Sciences, Andong National University)
Kim, Soon Young (Department of Biological Sciences, Andong National University)
Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University,)
Shin, Woo-Chang (Research Institute, Kooksoondang Brewery Co., Ltd.)
Kim, Jong-Sik (Department of Biological Sciences, Andong National University)
Publication Information
Journal of Life Science / v.26, no.1, 2016 , pp. 117-122 More about this Journal
Abstract
In the current study, the ethanol extracts and their subsequent organic solvent fractions from lees of sweet potato soju were prepared and the prepared samples were designated as from KSD-E8-1 to KSD-E8-5. Their effects on cell viability and nitric oxide (NO) production in mouse macrophage RAW 264.7 cells were investigated. The results showed that the ethyl acetate fraction (KSD-E8-3) of lees extracts from sweet potato soju significantly decreased nitric oxide (NO) production in LPS-activated RAW 264.7 cells, whereas they did not affect cell viabilities. The fraction KSD-E8-3 reduced the expression of pro-inflammatory genes such as COX-2, iNOS and TNF-alpha and also decreased protein expression of iNOS in a dose dependent manner, which were detected with RT-PCR and Western blot analysis, respectively. In addition, we detected the expression of mitogen-activated protein kinases (MAPKs) such as p38, JNK, and ERK1/2 and their phosphorylated forms. The results indicated that the treatment of the fraction KSD-E8-3 did inhibit phosphorylation of p38, JNK, and ERK1/2 MAPKs, indicating that the fraction KSD-E8-3 regulates LPS-induced inflammatory response via suppressing MAPK signaling pathway. Overall, these results may contribute to understand the molecular mechanism of anti-inflammatory effects by the ethyl acetate fraction of lees extracts from sweet potato soju.
Keywords
Anti-inflammation; lees extracts; organic solvent fractions; RAW 264.7 cell; sweet potato soju;
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1 Ju, A., Cho, Y. C. and Cho, S. 2015. Methanol extracts of Xanthium sibiricum roots inhibit inflammatory response via the inhibition of nuclear factor-κB (NF-κB) and signal transducer and activator of transctiption 3 (STAT3) in murine macrophage. J. Ethnopharmacol. DOI: 10.1016/j.jep.2015.07.038.   DOI
2 Akira, S. and Takeda, K. 2004. Toll-like receptor signaling. Nat. Rev. Immunol. 4, 499-511.   DOI
3 Eisinger, A. L., Prescott, S. M., Jones, D. A. and Stafforini, D. M. 2007. The role of cyclooxygenase-2 and prostaglandins in colon cancer. Prostaglandins Other Lipid Mediat. 82, 147-154.   DOI
4 Jacobsen, M. C., Dusart, P. J., Korowicz, K., Bajaj-eliott, M., Hart, S. L., Klein, N. J. and Dixon, G. L. 2015. A critical role for ATF2 transcription factor in the regulation of E-selectin expression in response to non-endotoxin components of Neisseria meningitides. Cell. Microbiol. DOI: 10.1111/cmi.12483.   DOI
5 Jin, J. H., Kim, J. S., Kang, S. S., Son, K. H., Chang, H. W. and Kim, H. P. 2010. Anti-inflammatory and anti-arthritic activity of the roots of sophora flavescens. J. Ethnopharmacol. 127, 589-595.   DOI
6 Kang, H. T., Lee, S. H., Kim, S. Y., Kim, M. S., Shin, W. C., Sohn, H. Y. and Kim, J. S. 2014. Anti-proliferative activities of solvent fractions of lees extracts in human colorectal HCT 116 ells. J. Life Sci. 24, 967-972.   DOI
7 Kim, H. G., Shi, C., Bode, A. M. and Dong, Z. 2015. p38α MAPK is required for arsenic-induced cell transformation. Mol. Carcinog. DOI: 10.1002/mc.22331.   DOI
8 Li, G., Wulan, H., Song, Z., Paik, P. A., Tsao, M. L., Goodman, G. M., MacEachern, P. T., Downey, R. S., Jankowska, A. J., Rabinowitz, Y. M., Learch, T. B., Song, D. Z., Yuan, J. J., Zheng, S. and Zheng, Z. 2015. Regulatory B cell function is suprresed by smoking and obesity in H. pylori-infected subjects and is correlated with elevated risk of gastric cancer. Plos One 10, e0134591.   DOI
9 Kim, T. Y., Jeon, T. W., Yeo, S. H., Kim, S. B., Kim, J. S. and Kwak, J. S. 2010. Antimicrobial, antioxidant and SOD-like activity effect of Jubak extracts. Kor. J. Food Nutr. 23, 299-305.
10 Kojda, G. and Harrison, D. 1999. Interactions between NO and reactive oxygen speices : pathophysiological importance in atherosclerosis, hypertension, diabetes and heart failure. Cardiovasc. Res. 43, 562-571.   DOI
11 Lee, E. S., Ju, H. K., Moon, T. C., Lee, E., Jahng, Y., Lee, S. H. and Chang, H. W. 2004. Inhibition of nitric oxide and tumor necrosis factor-alpha (TNF-alpha) production by prepenone compound through blockade of nuclear factor (NF)-κB activation in cultured murine macrophages. Biol. Pharm. Bull. 27, 617-620.   DOI
12 Lo, J. Y., Kamarudin, M. N., Hamdi, O. A., Awang, K. and Kadir, H. A. 2015. Curcumenol isolated from Curuma zedoaria suppresses Akt-mediated NF-κB activation and p38 MAPK signaling pathway in LPS-stimulated BV-2 microlial cells. Food Funct. 6, 3550-3559.   DOI
13 Anderson, N. and Borlak, J. 2008. Molecular mechanisms and therapeutic targets in steatosis and steatoheptitis. Pharmacol. Rev. 60, 311-357.   DOI
14 Chelsky, Z. L., Yue, P., Kondratyuk, T. P., Paladino, D., Pezzuto, J. M., Cushman, M. and Turkson, J. 2015. A resveratrol analogue promotes ERK MAPK-dependent STAT3 serine and tyrosine phosphorylation alterations and antitumor effects in vitro against human tumor cells. Mol. Pharmacol. 88, 524-533.   DOI
15 Xu, C., Chen, G., Yang, W., Xu, Y., Xu, Y., Huang, X., Liu, J., Feng, Y., Xu, Y. and Liu, B. 2015. Hyaluronan ameliorates LPS-induced acute lung injury in mice via Toll-like receptor (TLR) 4-dependent signaling pathway. Int. Immunopharmacol. 28, 1050-1058.   DOI
16 Sahu, B. D., Kumar, J. M. and Sistla, R. 2015. Baicalein, a bioflavonoid, prevents cisplatin-induced acute kidney injury by up-regulating antioxidant defense and down-regulating the MAPKs and NF-κB pathways. Plos One 10, e0134139.   DOI
17 Syed Hussein, S. S., Kamarudin, M. N. and Kadir, H. A. 2015. (+)-Catechin attenuates NF-κB activation through regulation of Akt, MAPK, and AMPK signaling pathways in LPS-induced BV-2 microglial cells. Am. J. Chin. Med. 43, 927-952.   DOI
18 Vlkova, M., Ticha, O., Nechvatalova, J., Kalina, T., Litzman, J., Mauri, C. and Blair, P. A. 2015. Regulatory B cells in CVID patients fail to suppress multifunctional IFN-gamma+TNF-alpha+CD4+ T cells differentiation. Clin. Immunol. 29, 292-300.
19 Kim, M. S., Lee, Y, S., Kim, J. S., Shin, W. C. and Sohn, H. Y. 2014. Evaluation of in-vitro antithrombosis activity of lees of Korean traditional wine. J. Life Sci. 24, 865-872.   DOI
20 Kindt, T. J., Goldsby, R. A. and Osborne, B. A. 2007. Innate immunity : Tenneys. Kuby Immunology. New York. Freeman Press 6, 52-73.
21 Monick, M. M., Arter, A. B., Robeff, P. K., Flaherty, D. M., Peterson, M. W. and Hunninghake, G. W. 2001. Lipopolysaccharide activates Akt in human alveolar macrophages resulting in nuclear accumulation and transcriptional activity of beta-catenin. J. Immunol. 166, 4713-4720.   DOI
22 Park, M. J., Kang, H. T., Kim, M. S., Shin, W. C., Sohn, H. Y. and Kim, J. S. 2014. Anti-inflammatory effects of extracts and their solvent fractions of rice wine lees. J. Life Sci. 24, 843-850.   DOI