Browse > Article
http://dx.doi.org/10.5352/JLS.2015.25.4.390

Effect of Gibberellin on the Adventitious Root Formation from the Leaves-derived Calli in Persicaria perfoliata  

Kim, Hyeon (Department of Biological Sciences, Dankook University)
Cha, Hyeon-Cheol (Department of Biological Sciences, Dankook University)
Publication Information
Journal of Life Science / v.25, no.4, 2015 , pp. 390-396 More about this Journal
Abstract
This study was carried out to investigate the action of phytohormones which influence the adventitious root formation of calli originating from the leaves of Persicaria perfoliata. The optimal medium condition for callus formation was ½-strength MS, 1% sucrose, and 4.5 μM 2,4-D. In order to determine which phytohormones had an effect on the adventitious root formation, the calluses were cultured in various media with different kinds of phytohormones. As a result, the medium with GA3 or IAA was shown to induce root formation. To deeply investigate the effects of GA3 and IAA, calli were cultured in 0.1, 1, and 10 mg/l levels of phytohormones. Numbers of roots formed per callus were 10.9, 14.2, 22.6 in GA3, 5.8, 3.9, 1.1 in IAA, respectively. Therefore, the higher GA3 or the lower IAA concentration, the more roots formed. To confirm this role of GA3 we tested with inhibitors PBZ and NPA. GA3 with PBZ resulted in reduction by 52.4~69.4% compared to GA3 alone. In contrast, GA3 with NPA resulted in an increase by -8~45.6% compared to GA3 alone in root formation. Also, results were determined on the effect of GA3 with other phytohormones on root formation. Kinetin, 2iP and ABA with GA3 had a negative effect, but IAA with GA3 showed a similar result to GA3 alone. From these results we infer GA plays a key role and auxin has subsidiary activity on adventitious root formation. This is the first report that indicates GA3 promotes adventitious root formation from calli in P. perfoliata.
Keywords
Adventitious root; callus; giberellin; Persicaria perfoliata; phytohormone inhibitor;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Fu, X. and Harberd, N. P. 2003. Auxin promotes Arabidopsis root growth by modulating gibberellin response. Nature 421, 740-743.   DOI
2 Aloni, R., Aloni, E., Lanqhans, M. and Ullrich, C.I. 2006. Role of auxin in regulating Arabidopsis flower development. Planta 223, 315-328.   DOI
3 Brian, P. W., Hemming, H. G. and Lowe, D. 1960. Inhibition of rooting of cuttings by gibberellic acid: with one figure in the text. Annal. Bot. 24, 407-419.   DOI
4 Caboni, E., Tonelli, M. G., Lauri, P., Iacovacci, P., Kevers, C., Damiano, C. and Gaspar, T. 1997. Biochemical aspects of almond microcuttings related to in vitro rooting ability. Biol. Plant 39, 91-97.   DOI
5 Kaneko, M., Inukai, Y., Ueguchi-Tanaka, M., Itoh, H., Izawa, T., Kobayashi, Y., Hattori, T., Miyao, A., Hirochika, H., Ashikari, M. and Matsuoka, M. 2004. Loss-of-function mutations of the rice GAMYB gene impair α-amylase expression in aleurone and flower development. Plant Cell 16, 33-44.   DOI
6 Kende, H. and Zeevaart, J. 1997. The Five “Classical” Plant Hormones. Plant Cell 9, 1197-1210.   DOI
7 Pamfil, D. and Bellini, C. 2011. Auxin control in the formation of adventitious roots. Not. Bot. Hort. Agrobot. Cluj. 39, 307-316.   DOI
8 Mattsson, J., Ckurshumova, W. and Berleth, T. 2003. Auxin signaling in Arabidopsis leaf vascular development. Plant Physiol. 131, 1327-1339.   DOI
9 Mauriat, M., Petterle, A., Bellini, C. and Moritz, T. 2014. Gibberellins inhibit adventitious rooting in hybrid aspen and Arabidopsis by affecting auxin transport. Plant J. 78, 372-384.   DOI
10 Overvoorde, P., Fukaki, H. and Beeckman, T. 2010. Auxin Control of Root Development. Cold Spring Harb. Perspect. Biol. 2, a001537.
11 Richards, D. E., King, K. E., Ait-ali, T. and Harberd, N. P. 2001. How gibberellin regulates plant growth and development: a molecular genetic analysis of gibberellin signaling. Ann. Rev. Plant Physiol. Plant Mol. Biol. 52, 67-88.   DOI
12 Riov, J. and Yang, S. F. 1989. Ethylene and auxin-ethylene interaction in adventitious root formation in mung bean (Vigna radiata) cuttings. J. Plant Growth Regul. 8, 131-141.   DOI
13 Seo, M., Nambara, E., Choi, G. and Yamaguchi, S. 2009. Interaction of light and hormone signals in germinating seeds. Plant Mol. Biol. 69, 463-472.   DOI
14 Sun, T. P. 2011, The molecular mechanism and evolution of the GA–GID1–DELLA signaling module in plants. Curr. Biol. 21, 338-345.   DOI
15 Taiz, L. and Zeiger, E. 2013. Plant Physiology, pp. 523-593, 5th ed., Sinauer Associates, Massachusetts, USA.
16 Li, F., Cui, X., Feng, Z., Du, X. and Zhu, J. 2012. The effect of 2,4-D and kinetin on dedifferentiation of petiole cells in Arabidopsis thaliana. Biol. Plant 56, 121-125.   DOI
17 Wilmoth, J. C., Wang, S., Tiwari, S. B., Joshi, A. D., Hagen, G., Guilfoyle, T. J., Alonsi, J. M., Ecker, J. R. and Reed, J. W. 2005. NPH4/ARF7 and ARF19 promote leaf expansion and auxin-induced lateral root formation. Plant J. 43, 118-130   DOI
18 Xu, M., Zhu, L., Shou, H. and Wu, P. 2005. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol. 46, 1674-1681.   DOI
19 Yang, Y. J., Kim, H. J., Kang, S. H. and Kang, S. C. 2011. Screening of natural herb resources for anti-oxidative effects in Korea. Kor. J. Plant Res. 24, 1-9.   DOI